Interleukin-1β gene (IL-1B) and interleukin 1 receptor antagonist gene (IL-1RN) polymorphisms and gastric cancer risk in an Omani Arab population

Mansour S. Al-Moundhri, Mariam Al-Nabhani, Bassim Al-Bahrani, Ikram A. Burney, Ali Al-Madhani, Shym S. Ganguly, Said A. Al-Yahyaee, Christopher S. Grant

Research output: Contribution to journalArticle

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Abstract

Background. Gastric cancer (GC) is the most common malignancy in Oman. Interleukin-1β gene (IL-1B) and interleukin-1 receptor antagonist gene (IL-1RN) polymorphisms have been associated with increased GC risk. No previous studies have examined their role in an Arab population. We tested the associations between polymorphisms of IL1B at positions -31, -511, and +3954 and the IL-1RN polymorphism [variable number of tandem repeats (VNTR) and TC polymorphism at the -2018 position] and GC in Omani Arab patients. Methods. Genomic DNA was extracted from peripheral blood of 245 control subjects and 118 gastric cancer patients. The DNA samples were analyzed using the TaqMan allelic discrimination test for IL-1B -31, -511, and +3954 polymorphisms and IL-1RN -2018 polymorphism. The VNTR of IL-1RN was genotyped using the polymerase chain reaction followed by agarose gel electrophoresis. Results. There was an association between the presence of IL-1RN*2 allele and gastric cancer [odds ratio (OR) = 2.2, 95% confidence interval (CI) = 1.0-3.3, P = 0.04). The GC risk further increased to OR = 3.5 (95% CI = 1.0-11.9) in Helicobacter pylori-positive patients. No association was found between any of the other polymorphisms studied and GC. Conclusion. IL-1RN polymorphism increased the risk of GC in an Omani Arab population, consistent with previous reports. In contrast, the IL-1B -31 polymorphism was not associated with an increased GC risk. These findings underscore the role of cytokine gene polymorphisms in the development of GC and further support the ethnic differences in the effect of IL-1B polymorphism on GC carcinogenesis.

Original languageEnglish
Pages (from-to)284-290
Number of pages7
JournalGastric Cancer
Volume9
Issue number4
DOIs
Publication statusPublished - Nov 2006

Fingerprint

Interleukin-1 Receptors
Interleukin-1
Stomach Neoplasms
Population
Genes
Minisatellite Repeats
Odds Ratio
Confidence Intervals
Oman
Agar Gel Electrophoresis
DNA
Helicobacter pylori
Carcinogenesis
Alleles
Cytokines

Keywords

  • Arab
  • Gastric cancer
  • IL-1B
  • IL-1RN
  • Omani
  • Polymorphism

ASJC Scopus subject areas

  • Oncology
  • Gastroenterology
  • Cancer Research

Cite this

Interleukin-1β gene (IL-1B) and interleukin 1 receptor antagonist gene (IL-1RN) polymorphisms and gastric cancer risk in an Omani Arab population. / Al-Moundhri, Mansour S.; Al-Nabhani, Mariam; Al-Bahrani, Bassim; Burney, Ikram A.; Al-Madhani, Ali; Ganguly, Shym S.; Al-Yahyaee, Said A.; Grant, Christopher S.

In: Gastric Cancer, Vol. 9, No. 4, 11.2006, p. 284-290.

Research output: Contribution to journalArticle

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title = "Interleukin-1β gene (IL-1B) and interleukin 1 receptor antagonist gene (IL-1RN) polymorphisms and gastric cancer risk in an Omani Arab population",
abstract = "Background. Gastric cancer (GC) is the most common malignancy in Oman. Interleukin-1β gene (IL-1B) and interleukin-1 receptor antagonist gene (IL-1RN) polymorphisms have been associated with increased GC risk. No previous studies have examined their role in an Arab population. We tested the associations between polymorphisms of IL1B at positions -31, -511, and +3954 and the IL-1RN polymorphism [variable number of tandem repeats (VNTR) and TC polymorphism at the -2018 position] and GC in Omani Arab patients. Methods. Genomic DNA was extracted from peripheral blood of 245 control subjects and 118 gastric cancer patients. The DNA samples were analyzed using the TaqMan allelic discrimination test for IL-1B -31, -511, and +3954 polymorphisms and IL-1RN -2018 polymorphism. The VNTR of IL-1RN was genotyped using the polymerase chain reaction followed by agarose gel electrophoresis. Results. There was an association between the presence of IL-1RN*2 allele and gastric cancer [odds ratio (OR) = 2.2, 95{\%} confidence interval (CI) = 1.0-3.3, P = 0.04). The GC risk further increased to OR = 3.5 (95{\%} CI = 1.0-11.9) in Helicobacter pylori-positive patients. No association was found between any of the other polymorphisms studied and GC. Conclusion. IL-1RN polymorphism increased the risk of GC in an Omani Arab population, consistent with previous reports. In contrast, the IL-1B -31 polymorphism was not associated with an increased GC risk. These findings underscore the role of cytokine gene polymorphisms in the development of GC and further support the ethnic differences in the effect of IL-1B polymorphism on GC carcinogenesis.",
keywords = "Arab, Gastric cancer, IL-1B, IL-1RN, Omani, Polymorphism",
author = "Al-Moundhri, {Mansour S.} and Mariam Al-Nabhani and Bassim Al-Bahrani and Burney, {Ikram A.} and Ali Al-Madhani and Ganguly, {Shym S.} and Al-Yahyaee, {Said A.} and Grant, {Christopher S.}",
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T1 - Interleukin-1β gene (IL-1B) and interleukin 1 receptor antagonist gene (IL-1RN) polymorphisms and gastric cancer risk in an Omani Arab population

AU - Al-Moundhri, Mansour S.

AU - Al-Nabhani, Mariam

AU - Al-Bahrani, Bassim

AU - Burney, Ikram A.

AU - Al-Madhani, Ali

AU - Ganguly, Shym S.

AU - Al-Yahyaee, Said A.

AU - Grant, Christopher S.

PY - 2006/11

Y1 - 2006/11

N2 - Background. Gastric cancer (GC) is the most common malignancy in Oman. Interleukin-1β gene (IL-1B) and interleukin-1 receptor antagonist gene (IL-1RN) polymorphisms have been associated with increased GC risk. No previous studies have examined their role in an Arab population. We tested the associations between polymorphisms of IL1B at positions -31, -511, and +3954 and the IL-1RN polymorphism [variable number of tandem repeats (VNTR) and TC polymorphism at the -2018 position] and GC in Omani Arab patients. Methods. Genomic DNA was extracted from peripheral blood of 245 control subjects and 118 gastric cancer patients. The DNA samples were analyzed using the TaqMan allelic discrimination test for IL-1B -31, -511, and +3954 polymorphisms and IL-1RN -2018 polymorphism. The VNTR of IL-1RN was genotyped using the polymerase chain reaction followed by agarose gel electrophoresis. Results. There was an association between the presence of IL-1RN*2 allele and gastric cancer [odds ratio (OR) = 2.2, 95% confidence interval (CI) = 1.0-3.3, P = 0.04). The GC risk further increased to OR = 3.5 (95% CI = 1.0-11.9) in Helicobacter pylori-positive patients. No association was found between any of the other polymorphisms studied and GC. Conclusion. IL-1RN polymorphism increased the risk of GC in an Omani Arab population, consistent with previous reports. In contrast, the IL-1B -31 polymorphism was not associated with an increased GC risk. These findings underscore the role of cytokine gene polymorphisms in the development of GC and further support the ethnic differences in the effect of IL-1B polymorphism on GC carcinogenesis.

AB - Background. Gastric cancer (GC) is the most common malignancy in Oman. Interleukin-1β gene (IL-1B) and interleukin-1 receptor antagonist gene (IL-1RN) polymorphisms have been associated with increased GC risk. No previous studies have examined their role in an Arab population. We tested the associations between polymorphisms of IL1B at positions -31, -511, and +3954 and the IL-1RN polymorphism [variable number of tandem repeats (VNTR) and TC polymorphism at the -2018 position] and GC in Omani Arab patients. Methods. Genomic DNA was extracted from peripheral blood of 245 control subjects and 118 gastric cancer patients. The DNA samples were analyzed using the TaqMan allelic discrimination test for IL-1B -31, -511, and +3954 polymorphisms and IL-1RN -2018 polymorphism. The VNTR of IL-1RN was genotyped using the polymerase chain reaction followed by agarose gel electrophoresis. Results. There was an association between the presence of IL-1RN*2 allele and gastric cancer [odds ratio (OR) = 2.2, 95% confidence interval (CI) = 1.0-3.3, P = 0.04). The GC risk further increased to OR = 3.5 (95% CI = 1.0-11.9) in Helicobacter pylori-positive patients. No association was found between any of the other polymorphisms studied and GC. Conclusion. IL-1RN polymorphism increased the risk of GC in an Omani Arab population, consistent with previous reports. In contrast, the IL-1B -31 polymorphism was not associated with an increased GC risk. These findings underscore the role of cytokine gene polymorphisms in the development of GC and further support the ethnic differences in the effect of IL-1B polymorphism on GC carcinogenesis.

KW - Arab

KW - Gastric cancer

KW - IL-1B

KW - IL-1RN

KW - Omani

KW - Polymorphism

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JO - Gastric Cancer

JF - Gastric Cancer

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