Interactions of intrinsically disordered Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 with membranes - Synergistic effects of lipid composition and temperature on secondary structure

Luna N. Rahman, Lin Chen, Sumaiya Nazim, Vladimir V. Bamm, Mahmoud W. Yaish, Barbara A. Moffatt, John R. Dutcher, George Harauz

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Dehydrins are intrinsically disordered (unstructured) proteins that are expressed in plants experiencing stressful conditions such as drought or low temperature. Dehydrins are typically found in the cytosol and nucleus, but also associate with chloroplasts, mitochondria, and the plasma membrane. Although their role is not completely understood, it has been suggested that they stabilize proteins or membrane structures during environmental stress, the latter association mediated by formation of amphipathic α-helices by conserved regions called the K-segments. Theilungieila salsuginea is a crucifer that thrives in the Canadian sub-Arctic (Yukon Territory) where it grows on saline-rich soils and experiences periods of both extreme cold and drought. We have cloned and expressed in Escherichia coli two dehydrins from this plant, denoted TsDHN-1 (acidic) and TsDHN-2 (basic). Here, we show using transmission-Fourier transform infrared (FTIR) spectroscopy that ordered secondary structure is induced and stabilized in these proteins by association with large unilamellar vesicles emulating the lipid compositions of plant plasma and organellar membranes. Moreover, this induced folding is enhanced at low temperatures, lending credence to the hypothesis that dehydrins stabilize plant outer and organellar membranes in conditions of cold.

Original languageEnglish
Pages (from-to)791-807
Number of pages17
JournalBiochemistry and Cell Biology
Volume88
Issue number5
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Intrinsically Disordered Proteins
Drought
Droughts
Yukon Territory
Extreme Cold
Cell Membrane
Association reactions
Membranes
Lipids
Unilamellar Liposomes
Membrane structures
Mitochondria
Temperature
Fourier Transform Infrared Spectroscopy
Chloroplasts
Cell membranes
Chemical analysis
Cytosol
Escherichia coli
Membrane Proteins

Keywords

  • CD spectroscopy
  • Cold tolerance
  • Dehydrins
  • FTIR spectroscopy
  • Induced folding
  • Intrinsically disordered protein (IDP)
  • Late embryogenesis abundant (LEA)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Interactions of intrinsically disordered Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 with membranes - Synergistic effects of lipid composition and temperature on secondary structure. / Rahman, Luna N.; Chen, Lin; Nazim, Sumaiya; Bamm, Vladimir V.; Yaish, Mahmoud W.; Moffatt, Barbara A.; Dutcher, John R.; Harauz, George.

In: Biochemistry and Cell Biology, Vol. 88, No. 5, 10.2010, p. 791-807.

Research output: Contribution to journalArticle

Rahman, Luna N. ; Chen, Lin ; Nazim, Sumaiya ; Bamm, Vladimir V. ; Yaish, Mahmoud W. ; Moffatt, Barbara A. ; Dutcher, John R. ; Harauz, George. / Interactions of intrinsically disordered Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 with membranes - Synergistic effects of lipid composition and temperature on secondary structure. In: Biochemistry and Cell Biology. 2010 ; Vol. 88, No. 5. pp. 791-807.
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