Abstract
Four strains of aerobic heterotrophic bacteria were isolated on crude oil with the aim to test whether their presence and activity might support the growth of cyanobacteria in oil-polluted microbial mats and whether the cyanobacterial exudates might play a role in stimulating their degradative activities. The strains were phylogenetically related to known oil-degrading species from the genera Marinobacter, Pseudomonas and Sphingomonas. Three strains (GM41, GM61 and GM63) grew well on C5-C18 alkanes but not on 7 tested aromatics, whereas one strain (i.e. GM42) grew best on phenanthrene and pentane. All strains showed ability to metabolize a range of cyanobacterial photosynthetic and fermentative exudates. In coculture experiments, the addition of the Pseudomonas-related GM41 strain to the cyanobacterium Synechocystis PCC6803, found in the same mat, resulted in 8-fold increase in the cyanobacterial biomass. This growth was more pronounced when hexadecane was added to the culture medium. The addition of representative substrates of cyanobacterial exudates to the phenanthrene-degrading strain GM42 resulted in variable effects. While acetate, pyruvate and glucose enhanced phenanthrene degradation, alanine and butanol showed no effect. We conclude that aerobic heterotrophic bacteria-cyanobacteria consortia can be very useful for bioremediating oil-polluted sites, circumventing the costly use of organic and inorganic fertilizers.
| Original language | English |
|---|---|
| Pages (from-to) | 58-64 |
| Number of pages | 7 |
| Journal | International Biodeterioration and Biodegradation |
| Volume | 64 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2010 |
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Keywords
- Aerobic heterotrophic bacteria
- Cyanobacteria
- Cyanobacterial exudates
- Cyanobacterial mats
- Hydrocarbons
ASJC Scopus subject areas
- Waste Management and Disposal
- Microbiology
- Biomaterials
Cite this
Interaction between cyanobacteria and aerobic heterotrophic bacteria in the degradation of hydrocarbons. / Abed, R. M M.
In: International Biodeterioration and Biodegradation, Vol. 64, No. 1, 01.2010, p. 58-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Interaction between cyanobacteria and aerobic heterotrophic bacteria in the degradation of hydrocarbons
AU - Abed, R. M M
PY - 2010/1
Y1 - 2010/1
N2 - Four strains of aerobic heterotrophic bacteria were isolated on crude oil with the aim to test whether their presence and activity might support the growth of cyanobacteria in oil-polluted microbial mats and whether the cyanobacterial exudates might play a role in stimulating their degradative activities. The strains were phylogenetically related to known oil-degrading species from the genera Marinobacter, Pseudomonas and Sphingomonas. Three strains (GM41, GM61 and GM63) grew well on C5-C18 alkanes but not on 7 tested aromatics, whereas one strain (i.e. GM42) grew best on phenanthrene and pentane. All strains showed ability to metabolize a range of cyanobacterial photosynthetic and fermentative exudates. In coculture experiments, the addition of the Pseudomonas-related GM41 strain to the cyanobacterium Synechocystis PCC6803, found in the same mat, resulted in 8-fold increase in the cyanobacterial biomass. This growth was more pronounced when hexadecane was added to the culture medium. The addition of representative substrates of cyanobacterial exudates to the phenanthrene-degrading strain GM42 resulted in variable effects. While acetate, pyruvate and glucose enhanced phenanthrene degradation, alanine and butanol showed no effect. We conclude that aerobic heterotrophic bacteria-cyanobacteria consortia can be very useful for bioremediating oil-polluted sites, circumventing the costly use of organic and inorganic fertilizers.
AB - Four strains of aerobic heterotrophic bacteria were isolated on crude oil with the aim to test whether their presence and activity might support the growth of cyanobacteria in oil-polluted microbial mats and whether the cyanobacterial exudates might play a role in stimulating their degradative activities. The strains were phylogenetically related to known oil-degrading species from the genera Marinobacter, Pseudomonas and Sphingomonas. Three strains (GM41, GM61 and GM63) grew well on C5-C18 alkanes but not on 7 tested aromatics, whereas one strain (i.e. GM42) grew best on phenanthrene and pentane. All strains showed ability to metabolize a range of cyanobacterial photosynthetic and fermentative exudates. In coculture experiments, the addition of the Pseudomonas-related GM41 strain to the cyanobacterium Synechocystis PCC6803, found in the same mat, resulted in 8-fold increase in the cyanobacterial biomass. This growth was more pronounced when hexadecane was added to the culture medium. The addition of representative substrates of cyanobacterial exudates to the phenanthrene-degrading strain GM42 resulted in variable effects. While acetate, pyruvate and glucose enhanced phenanthrene degradation, alanine and butanol showed no effect. We conclude that aerobic heterotrophic bacteria-cyanobacteria consortia can be very useful for bioremediating oil-polluted sites, circumventing the costly use of organic and inorganic fertilizers.
KW - Aerobic heterotrophic bacteria
KW - Cyanobacteria
KW - Cyanobacterial exudates
KW - Cyanobacterial mats
KW - Hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=73349123436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=73349123436&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2009.10.008
DO - 10.1016/j.ibiod.2009.10.008
M3 - Article
VL - 64
SP - 58
EP - 64
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
SN - 0964-8305
IS - 1
ER -