Insights into the molecular interaction between sucrose and α-chymotrypsin

Sadegh Farhadian, Behzad Shareghi, Lida Momeni, Osama K. Abou-Zied, Vladimir A. Sirotkin, Masanori Tachiya, Ali A. Saboury

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

One of the most important purposes of enzyme engineering is to increase the thermal and kinetic stability of enzymes, which is an important factor for using enzymes in industry. The purpose of the present study is to achieve a higher thermal stability of α-chymotrypsin (α-Chy) by modification of the solvent environment. The influence of sucrose was investigated using thermal denaturation analysis, fluorescence spectroscopy, circular dichroism, molecular docking and molecular dynamics (MD) simulations. The results point to the effect of sucrose in enhancing the α-Chy stability. Fluorescence spectroscopy revealed one binding site that is dominated by static quenching. Molecular docking and MD simulation results indicate that hydrogen bonding and van der Waals forces play a major role in stabilizing the complex. Tm of this complex was enhanced due to the higher H-bond formation and the lower surface hydrophobicity after sucrose modification. The results show the ability of sucrose in protecting the native structural conformation of α-Chy. Sucrose was preferentially excluded from the surface of α-Chy which is explained by the higher tendency of water toward favorable interactions with the functional groups of α-Chy than with sucrose.

Original languageEnglish
Pages (from-to)950-960
Number of pages11
JournalInternational Journal of Biological Macromolecules
Volume114
DOIs
Publication statusPublished - Jul 15 2018

Fingerprint

Molecular interactions
Chymotrypsin
Sucrose
Hot Temperature
Fluorescence Spectrometry
Fluorescence spectroscopy
Molecular Dynamics Simulation
Molecular dynamics
Enzymes
Enzyme Stability
Van der Waals forces
Denaturation
Computer simulation
Hydrogen Bonding
Hydrophobicity
Circular Dichroism
Hydrophobic and Hydrophilic Interactions
Functional groups
Conformations
Quenching

Keywords

  • Circular dichroism
  • Fluorescence spectroscopy
  • Molecular docking and dynamics
  • Sucrose
  • α-Chymotrypsin

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Insights into the molecular interaction between sucrose and α-chymotrypsin. / Farhadian, Sadegh; Shareghi, Behzad; Momeni, Lida; Abou-Zied, Osama K.; Sirotkin, Vladimir A.; Tachiya, Masanori; Saboury, Ali A.

In: International Journal of Biological Macromolecules, Vol. 114, 15.07.2018, p. 950-960.

Research output: Contribution to journalArticle

Farhadian, Sadegh ; Shareghi, Behzad ; Momeni, Lida ; Abou-Zied, Osama K. ; Sirotkin, Vladimir A. ; Tachiya, Masanori ; Saboury, Ali A. / Insights into the molecular interaction between sucrose and α-chymotrypsin. In: International Journal of Biological Macromolecules. 2018 ; Vol. 114. pp. 950-960.
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