Inhibition of marine biofouling by bacterial quorum sensing inhibitors

Sergey Dobretsov, Max Teplitski, Mirko Bayer, Sarath Gunasekera, Peter Proksch, Valerie J. Paul

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Seventy eight natural products from chemical libraries containing compounds from marine organisms (sponges, algae, fungi, tunicates and cyanobacteria) and terrestrial plants, were screened for the inhibition of bacterial quorum sensing (QS) using a reporter strain Chromobacterium violaceum CV017. About half of the natural products did not show any QS inhibition. Twenty four percent of the tested compounds inhibited QS of the reporter without causing toxicity. The QS inhibitory activities of the most potent and abundant compounds were further investigated using the LuxR-based reporter E. coli pSB401 and the LasR-based reporter E. coli pSB1075. Midpacamide and tenuazonic acid were toxic to the tested reporters. QS-dependent luminescence of the LasR-based reporter, which is normally induced by N-3-oxo-dodecanoyl-L-homoserine lactone, was reduced by demethoxy encecalin and hymenialdisin at concentrations >6.6 μM and 15 μM, respectively. Hymenialdisin, demethoxy encecalin, microcolins A and B and kojic acid inhibited responses of the LuxR-based reporter induced by N-3-oxo-hexanoyl-L-homoserine lactone at concentrations >0.2 μM, 2.2 μM, 1.5 μM, 15 μM and 36 μM, respectively. The ability to prevent microfouling by one of the compounds screened in this study (kojic acid; final concentrations 330 μM and 1 μM) was tested in a controlled mesocosm experiment. Kojic acid inhibited formation of microbial communities on glass slides, decreasing the densities of bacteria and diatoms in comparison with the control lacking kojic acid. The study suggests that natural products with QS inhibitory properties can be used for controlling biofouling communities.

Original languageEnglish
Pages (from-to)893-905
Number of pages13
JournalBiofouling
Volume27
Issue number8
DOIs
Publication statusPublished - Sep 2011

Fingerprint

Biofouling
biofouling
Quorum Sensing
quorum sensing
kojic acid
inhibitor
acid
Biological Products
homoserine
lactones
algae
Tenuazonic Acid
tenuazonic acid
Chromobacterium
Chromobacterium violaceum
Small Molecule Libraries
Escherichia coli
mesocosm
Diatoms
Urochordata

Keywords

  • Antifouling
  • Biofilms
  • Inhibitors
  • Natural products
  • Quorum sensing

ASJC Scopus subject areas

  • Aquatic Science
  • Water Science and Technology
  • Applied Microbiology and Biotechnology

Cite this

Dobretsov, S., Teplitski, M., Bayer, M., Gunasekera, S., Proksch, P., & Paul, V. J. (2011). Inhibition of marine biofouling by bacterial quorum sensing inhibitors. Biofouling, 27(8), 893-905. https://doi.org/10.1080/08927014.2011.609616

Inhibition of marine biofouling by bacterial quorum sensing inhibitors. / Dobretsov, Sergey; Teplitski, Max; Bayer, Mirko; Gunasekera, Sarath; Proksch, Peter; Paul, Valerie J.

In: Biofouling, Vol. 27, No. 8, 09.2011, p. 893-905.

Research output: Contribution to journalArticle

Dobretsov, S, Teplitski, M, Bayer, M, Gunasekera, S, Proksch, P & Paul, VJ 2011, 'Inhibition of marine biofouling by bacterial quorum sensing inhibitors', Biofouling, vol. 27, no. 8, pp. 893-905. https://doi.org/10.1080/08927014.2011.609616
Dobretsov, Sergey ; Teplitski, Max ; Bayer, Mirko ; Gunasekera, Sarath ; Proksch, Peter ; Paul, Valerie J. / Inhibition of marine biofouling by bacterial quorum sensing inhibitors. In: Biofouling. 2011 ; Vol. 27, No. 8. pp. 893-905.
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