Influence of iron, deferoxamine and ascorbic acid on gentamicin-induced nephrotoxicity in rats

T. H Ben Ismail, B. H. Ali, A. A. Bashir

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49 Citations (Scopus)

Abstract

1. Nephrotoxicity was induced in rats by injecting gentamicin intramuscularly (i.m.) at a dose of 80 mg/kg for 6 days. Treated animals demonstrated a typical pattern of nephrotoxicity characterized by increased serum creatinine and urea concentrations, and by necrosis of proxinal tubular epithelium. 2. Pretreatment of rats with iron (Fe3+) at daily i.m. doses of 2, 4 and 8 mg/kg for 14 days, with gentamicin given during the last 6 days of treatment, significantly potentiated the gentamicin-induced increases in creatinine and urea concentrations and exacerbated renal histological damage. 3. Gentamicin significantly increased serum Fe3+ concentration in rats treated with Fe3+ and gentamicin, compared to Fe3+-treated rats. 4. The Fe3+ antidote deferoxamine (100 mg/kg, i.m.) given with gentamicin was ineffective in antagonizing the potentiating effect of Fe3+ on gentamicine-induced nephrotoxicity. 5. Ascorbic acid (50 mg/kg, i.m. for 14 days) was ineffective in altering the nephrotoxicity of gentamincin (80 mg/kg) given during the last 6 days of treatment. At a dose of 100 mg/kg for 14 days, ascorbic acid significantly reduced gentamicin-induced increases in creatinine and urea levels, and ameliorated proximal tubular damage. However, at a dose of 200 mg/kg, ascorbic acid exacerbated gentamicin-induced increases in creatinine and urea levels and increased the severity of the histological damage.

Original languageEnglish
Pages (from-to)1249-1252
Number of pages4
JournalGeneral Pharmacology
Volume25
Issue number6
DOIs
Publication statusPublished - 1994

Keywords

  • ascorbic acid
  • Gentamicin
  • iron
  • nephrotoxicity
  • rats

ASJC Scopus subject areas

  • Pharmacology

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