TY - JOUR
T1 - Infectious clone construction and pathogenicity confirmation of Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) by southern blot analysis
AU - Arif, Muhammad
AU - ul Islam, Saif
AU - Alotaibi, Saqer S.
AU - Elshehawi, Ahmed M.
AU - Ahmed, Mohamed A.A.
AU - Al-Sadi, Abdullah M.
N1 - Funding Information:
The current work was funded by Taif University Researchers Supporting Project number (TURSP-2020/38), Taif University, Taif, Saudi Arabia. There was no additional external funding involved in the study. There were no additional external funding involved in the study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2021 Arif et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2021/5/14
Y1 - 2021/5/14
N2 - Geminiviruses are insect-transmissible, economically vital group of plant viruses, which cause significant losses to crop production and ornamental plants across the world. During this study, infectious clones of three devastating begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) were constructed by following novel protocol. All infectious clones were confirmed by cloning and sequencing. All of the infectious clones were agro-inoculated in Agrobacterium. After the agro-infiltrations, all clones were injected into Nicotiana benthamiana and jute plants under controlled condition. After 28 days of inoculation, plants exhibited typical symptoms of their corresponding viruses. All the symptomatic and asymptomatic leaves were collected from inoculated plants for further analysis. The southern blot analysis was used to confirm the infection of studied begomoviruses. At the end, all the products were sequenced and analyzed.
AB - Geminiviruses are insect-transmissible, economically vital group of plant viruses, which cause significant losses to crop production and ornamental plants across the world. During this study, infectious clones of three devastating begomoviruses, i.e., Cotton leaf curl Multan virus (CLCuMuV), Ramie mosaic virus (RamV) and Corchorus yellow vein Vietnam virus (CoYVV) were constructed by following novel protocol. All infectious clones were confirmed by cloning and sequencing. All of the infectious clones were agro-inoculated in Agrobacterium. After the agro-infiltrations, all clones were injected into Nicotiana benthamiana and jute plants under controlled condition. After 28 days of inoculation, plants exhibited typical symptoms of their corresponding viruses. All the symptomatic and asymptomatic leaves were collected from inoculated plants for further analysis. The southern blot analysis was used to confirm the infection of studied begomoviruses. At the end, all the products were sequenced and analyzed.
KW - Agrobacterium/virology
KW - Animals
KW - Begomovirus/genetics
KW - Crop Production/statistics & numerical data
KW - Crops, Agricultural/virology
KW - DNA, Viral/genetics
KW - Genome, Viral/genetics
KW - Insect Vectors/virology
KW - Plant Diseases/virology
KW - Sequence Analysis, DNA
KW - Tobacco/virology
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U2 - 10.1371/journal.pone.0251232
DO - 10.1371/journal.pone.0251232
M3 - Article
C2 - 33989327
AN - SCOPUS:85106332999
SN - 1932-6203
VL - 16
SP - e0251232
JO - PLoS One
JF - PLoS One
IS - 5
M1 - e0251232
ER -