Abstract
Aqueous extract (10%) from leaves of zimmu (Allium sativum L. x Allium cepa L.) when applied as foliar spray to first and second leaves of cotton plants induced systemic resistance in third and fourth leaves to a challenge infection with Xanthomonas campestris pv. malvacearum and reduced the number of lesions by up to 73% compared with water-treated control plants. The treated leaves exhibited significantly high activity of enzymes phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase along with rapid accumulation of phenolics. The activities of chitinase and β-1,3-glucanase were greatly elevated in treated plants as compared to water-treated controls. An 11-fold increase in chitinase activity was evident 4 d after treatment. Western blot analysis revealed that a chitinase with an apparent molecular weight of 58 kDa that cross-reacted with a barley chitinase antiserum was induced in cotton leaves 3 d after treatment and the maximum induction of this chitinase was detected 4 d after treatment. The present study provides evidence for the induction of biochemical defence mechanisms in cotton leaves after treatment with leaf extract from zimmu.
Original language | English |
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Pages (from-to) | 309-322 |
Number of pages | 14 |
Journal | Archives of Phytopathology and Plant Protection |
Volume | 40 |
Issue number | 5 |
DOIs | |
Publication status | Published - Oct 2007 |
Externally published | Yes |
Keywords
- Bacterial leaf blight
- Gossypium hirsutum
- Induced resistance
- Pathogenesis-related proteins
- Plant extract
ASJC Scopus subject areas
- Agronomy and Crop Science