Induction of systemic resistance to bacterial blight caused by Xanthomonas campestris pv. malvacearum in cotton by leaf extract from a medicinal plant zimmu (Allium sativum L. x Allium cepa L.)

V. K. Satya, S. Gayathiri, R. Bhaskaran, V. Paranidharan, R. Velazhahan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Aqueous extract (10%) from leaves of zimmu (Allium sativum L. x Allium cepa L.) when applied as foliar spray to first and second leaves of cotton plants induced systemic resistance in third and fourth leaves to a challenge infection with Xanthomonas campestris pv. malvacearum and reduced the number of lesions by up to 73% compared with water-treated control plants. The treated leaves exhibited significantly high activity of enzymes phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase along with rapid accumulation of phenolics. The activities of chitinase and β-1,3-glucanase were greatly elevated in treated plants as compared to water-treated controls. An 11-fold increase in chitinase activity was evident 4 d after treatment. Western blot analysis revealed that a chitinase with an apparent molecular weight of 58 kDa that cross-reacted with a barley chitinase antiserum was induced in cotton leaves 3 d after treatment and the maximum induction of this chitinase was detected 4 d after treatment. The present study provides evidence for the induction of biochemical defence mechanisms in cotton leaves after treatment with leaf extract from zimmu.

Original languageEnglish
Pages (from-to)309-322
Number of pages14
JournalArchives of Phytopathology and Plant Protection
Volume40
Issue number5
DOIs
Publication statusPublished - Oct 2007

Keywords

  • Bacterial leaf blight
  • Gossypium hirsutum
  • Induced resistance
  • Pathogenesis-related proteins
  • Plant extract

ASJC Scopus subject areas

  • Agronomy and Crop Science

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