Induction of systemic resistance to bacterial blight caused by Xanthomonas campestris pv. malvacearum in cotton by leaf extract from a medicinal plant zimmu (Allium sativum L. x Allium cepa L.)

V. K. Satya, S. Gayathiri, R. Bhaskaran, V. Paranidharan, R. Velazhahan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aqueous extract (10%) from leaves of zimmu (Allium sativum L. x Allium cepa L.) when applied as foliar spray to first and second leaves of cotton plants induced systemic resistance in third and fourth leaves to a challenge infection with Xanthomonas campestris pv. malvacearum and reduced the number of lesions by up to 73% compared with water-treated control plants. The treated leaves exhibited significantly high activity of enzymes phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase along with rapid accumulation of phenolics. The activities of chitinase and β-1,3-glucanase were greatly elevated in treated plants as compared to water-treated controls. An 11-fold increase in chitinase activity was evident 4 d after treatment. Western blot analysis revealed that a chitinase with an apparent molecular weight of 58 kDa that cross-reacted with a barley chitinase antiserum was induced in cotton leaves 3 d after treatment and the maximum induction of this chitinase was detected 4 d after treatment. The present study provides evidence for the induction of biochemical defence mechanisms in cotton leaves after treatment with leaf extract from zimmu.

Original languageEnglish
Pages (from-to)309-322
Number of pages14
JournalArchives of Phytopathology and Plant Protection
Volume40
Issue number5
DOIs
Publication statusPublished - Oct 2007

Fingerprint

Xanthomonas campestris pv. malvacearum
Allium sativum
Allium cepa
blight
leaf extracts
medicinal plants
chitinase
cotton
leaves
lesions (plant)
water
phenylalanine ammonia-lyase
catechol oxidase
defense mechanisms
antiserum
peroxidase
Western blotting
barley
enzyme activity
molecular weight

Keywords

  • Bacterial leaf blight
  • Gossypium hirsutum
  • Induced resistance
  • Pathogenesis-related proteins
  • Plant extract

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{1487afa61f024cfb83adb093f744e28e,
title = "Induction of systemic resistance to bacterial blight caused by Xanthomonas campestris pv. malvacearum in cotton by leaf extract from a medicinal plant zimmu (Allium sativum L. x Allium cepa L.)",
abstract = "Aqueous extract (10{\%}) from leaves of zimmu (Allium sativum L. x Allium cepa L.) when applied as foliar spray to first and second leaves of cotton plants induced systemic resistance in third and fourth leaves to a challenge infection with Xanthomonas campestris pv. malvacearum and reduced the number of lesions by up to 73{\%} compared with water-treated control plants. The treated leaves exhibited significantly high activity of enzymes phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase along with rapid accumulation of phenolics. The activities of chitinase and β-1,3-glucanase were greatly elevated in treated plants as compared to water-treated controls. An 11-fold increase in chitinase activity was evident 4 d after treatment. Western blot analysis revealed that a chitinase with an apparent molecular weight of 58 kDa that cross-reacted with a barley chitinase antiserum was induced in cotton leaves 3 d after treatment and the maximum induction of this chitinase was detected 4 d after treatment. The present study provides evidence for the induction of biochemical defence mechanisms in cotton leaves after treatment with leaf extract from zimmu.",
keywords = "Bacterial leaf blight, Gossypium hirsutum, Induced resistance, Pathogenesis-related proteins, Plant extract",
author = "Satya, {V. K.} and S. Gayathiri and R. Bhaskaran and V. Paranidharan and R. Velazhahan",
year = "2007",
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T1 - Induction of systemic resistance to bacterial blight caused by Xanthomonas campestris pv. malvacearum in cotton by leaf extract from a medicinal plant zimmu (Allium sativum L. x Allium cepa L.)

AU - Satya, V. K.

AU - Gayathiri, S.

AU - Bhaskaran, R.

AU - Paranidharan, V.

AU - Velazhahan, R.

PY - 2007/10

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N2 - Aqueous extract (10%) from leaves of zimmu (Allium sativum L. x Allium cepa L.) when applied as foliar spray to first and second leaves of cotton plants induced systemic resistance in third and fourth leaves to a challenge infection with Xanthomonas campestris pv. malvacearum and reduced the number of lesions by up to 73% compared with water-treated control plants. The treated leaves exhibited significantly high activity of enzymes phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase along with rapid accumulation of phenolics. The activities of chitinase and β-1,3-glucanase were greatly elevated in treated plants as compared to water-treated controls. An 11-fold increase in chitinase activity was evident 4 d after treatment. Western blot analysis revealed that a chitinase with an apparent molecular weight of 58 kDa that cross-reacted with a barley chitinase antiserum was induced in cotton leaves 3 d after treatment and the maximum induction of this chitinase was detected 4 d after treatment. The present study provides evidence for the induction of biochemical defence mechanisms in cotton leaves after treatment with leaf extract from zimmu.

AB - Aqueous extract (10%) from leaves of zimmu (Allium sativum L. x Allium cepa L.) when applied as foliar spray to first and second leaves of cotton plants induced systemic resistance in third and fourth leaves to a challenge infection with Xanthomonas campestris pv. malvacearum and reduced the number of lesions by up to 73% compared with water-treated control plants. The treated leaves exhibited significantly high activity of enzymes phenylalanine ammonia-lyase, peroxidase and polyphenol oxidase along with rapid accumulation of phenolics. The activities of chitinase and β-1,3-glucanase were greatly elevated in treated plants as compared to water-treated controls. An 11-fold increase in chitinase activity was evident 4 d after treatment. Western blot analysis revealed that a chitinase with an apparent molecular weight of 58 kDa that cross-reacted with a barley chitinase antiserum was induced in cotton leaves 3 d after treatment and the maximum induction of this chitinase was detected 4 d after treatment. The present study provides evidence for the induction of biochemical defence mechanisms in cotton leaves after treatment with leaf extract from zimmu.

KW - Bacterial leaf blight

KW - Gossypium hirsutum

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KW - Pathogenesis-related proteins

KW - Plant extract

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