Abstract
The Pseudomonas fluorescens isolate Pf1 was found to inhibit the growth of pathogen Alternaria palandui, in vitro. In the present study, foliar application of a talc-based formulation of Pf1 significantly reduced the incidence of leaf blight of onion, caused by A. palandui. Induction of defense-related proteins viz., chitinase, β-1,3 glucanase, peroxidase (PO) and polyphenol oxidase (PPO) by application of Pf1, was studied against A. palandui infection in resistant (IHR 56) and susceptible (MDU1) onion cultivars. Chitinase in both cultivars, with or without challenge-inoculation of A. palandui revealed changes in the isoform pattern. The Native-PAGE of PO showed induction of PO2 isoform in both the cultivars, in response to inoculation of pathogen. Isoform analysis of PPO also exhibited induction in the Pf1-treated plants challenged with pathogen. Similarly, the activity of β-1,3-glucanase was greatly induced in Pf1-treated plants, challenged with pathogen as compared to controls. Thus, the P. fluorescens-treated plants showed significant increase in the levels of the defense enzymes, in comparison to the plants challenged with the pathogen.
Original language | English |
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Pages (from-to) | 371-377 |
Number of pages | 7 |
Journal | Indian Journal of Biochemistry and Biophysics |
Volume | 42 |
Issue number | 6 |
Publication status | Published - Dec 2005 |
Externally published | Yes |
Keywords
- Alternaria palandui
- Chitinase
- Defense-related proteins
- Induced systemic resistance
- Leaf blight
- Onion
- Peroxidase
- Polyphenol oxidase
- Pseudomonas fluorescens
- β-1,3 glucanase
ASJC Scopus subject areas
- Biophysics
- Biochemistry