Induction of phenolics and defense-related enzymes in coconut (Cocos nucifera L.) roots treated with biocontrol agents

M. Karthikeyan, K. Radhika, S. Mathiyazhagan, R. Bhaskaran, R. Samiyappan, R. Velazhahan

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The effect of soil application of biocontrol agents (Pseudomonas fluorescens, Trichoderma viride and T. harzianum) in combination with chitin on induction of phenolics and defense enzymes in coconut roots infected with Ganoderma lucidum, the causal agent of Ganoderma disease, was investigated. Soil application of these biocontrol formulations in combination with chitin induced a significant increase in the activities of peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), chitinase and β-1,3-glucanase in the G. lucidum infected palms. Activities of both PAL and PO reached maximum levels within 3 d while the activity of PPO reached the maximum level 6 d after application of a mixture of P. fluorescens, T. viride and chitin. Isozyme analysis revealed that unique PO3 and PPO2 isozymes were induced in coconut palms treated with P. fluorescens + T. viride + chitin. Accumulation of phenolics was recorded 3 d after treatment and reached maximum levels 9 d after treatment application. Activity of chitinase was significantly increased from the third day after treatment imposition and continued to increase up to 9 to 12 d in all treatments. Chitinase isozyme analysis revealed that a unique Chit3 isoform was induced in coconut roots treated with P. fluorescens + T. viride + chitin. The β-1,3-glucanase activity was maximum 9 d after treatment application. The mechanisms by which P. fluorescens + T. viride + chitin reduced the incidence of Ganoderma disease in coconut may be related to its ability to induce defense mechanisms in coconut palms.

Original languageEnglish
Pages (from-to)367-377
Number of pages11
JournalBrazilian Journal of Plant Physiology
Volume18
Issue number3
DOIs
Publication statusPublished - 2006

Fingerprint

Cocos nucifera
coconuts
chitin
Trichoderma viride
biological control agents
Pseudomonas fluorescens
chitinase
enzymes
Ganoderma
Ganoderma lucidum
isozymes
phenylalanine ammonia-lyase
catechol oxidase
soil treatment
peroxidase
Trichoderma harzianum
defense mechanisms
biological control
incidence

Keywords

  • Chitin
  • Ganoderma disease
  • Induced systemic resistance
  • Pathogenesis-related proteins
  • Phenolics
  • Pseudomonas fluorescens
  • Trichoderma

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Induction of phenolics and defense-related enzymes in coconut (Cocos nucifera L.) roots treated with biocontrol agents. / Karthikeyan, M.; Radhika, K.; Mathiyazhagan, S.; Bhaskaran, R.; Samiyappan, R.; Velazhahan, R.

In: Brazilian Journal of Plant Physiology, Vol. 18, No. 3, 2006, p. 367-377.

Research output: Contribution to journalArticle

Karthikeyan, M. ; Radhika, K. ; Mathiyazhagan, S. ; Bhaskaran, R. ; Samiyappan, R. ; Velazhahan, R. / Induction of phenolics and defense-related enzymes in coconut (Cocos nucifera L.) roots treated with biocontrol agents. In: Brazilian Journal of Plant Physiology. 2006 ; Vol. 18, No. 3. pp. 367-377.
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AB - The effect of soil application of biocontrol agents (Pseudomonas fluorescens, Trichoderma viride and T. harzianum) in combination with chitin on induction of phenolics and defense enzymes in coconut roots infected with Ganoderma lucidum, the causal agent of Ganoderma disease, was investigated. Soil application of these biocontrol formulations in combination with chitin induced a significant increase in the activities of peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), chitinase and β-1,3-glucanase in the G. lucidum infected palms. Activities of both PAL and PO reached maximum levels within 3 d while the activity of PPO reached the maximum level 6 d after application of a mixture of P. fluorescens, T. viride and chitin. Isozyme analysis revealed that unique PO3 and PPO2 isozymes were induced in coconut palms treated with P. fluorescens + T. viride + chitin. Accumulation of phenolics was recorded 3 d after treatment and reached maximum levels 9 d after treatment application. Activity of chitinase was significantly increased from the third day after treatment imposition and continued to increase up to 9 to 12 d in all treatments. Chitinase isozyme analysis revealed that a unique Chit3 isoform was induced in coconut roots treated with P. fluorescens + T. viride + chitin. The β-1,3-glucanase activity was maximum 9 d after treatment application. The mechanisms by which P. fluorescens + T. viride + chitin reduced the incidence of Ganoderma disease in coconut may be related to its ability to induce defense mechanisms in coconut palms.

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