Induction of pathogenesis-related proteins, phenolics and phenylalanine ammonia-lyase in groundnut by Pseudomonas fluorescens

B. Meena, R. Radhajeyalakshmi, T. Marimuthu, P. Vidhyasekaran, S. Doraiswamy, R. Velazhahan

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Foliar application of a plant growth-promoting rhizobacterium, Pseudomonas fluorescens strain Pf1 significantly controlled late leaf spot and rust diseases of groundnut (Arachis hypogaea L.) under greenhouse conditions. Groundnut plants, when sprayed with P. fluorescensstrain Pf1, showed increase in activity of phenylalanine ammonia-lyase 1 day after application and the maximum enzyme activity was detected at 3 days after treatment. An increase in phenolic content was observed 1 day after P. fluorescens treatment. Also chitinase and β-1,3-glucanase activities increased significantly 1 day after P. fluorescens treatment and maximum activities were reached 4 days later. Application of P. fluorescens to groundnut leaves induced accumulation of a 23-kDa thaumatin-like protein (TLP) and a 30-kDa glucanase. The induction of 23-kDa TLP was detected only in treated leaves and not in roots, stems and petioles of the groundnut plants. The 30-kDa glucanase started to accumulate 1 day after treatment and the level of the protein continued to increase throughout the experimental period of 4 days. Both TLP and glucanase were found in the intercellular fluid of P. fluorescens-sprayed groundnut leaves.

Original languageEnglish
Pages (from-to)514-527
Number of pages14
JournalZeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
Volume107
Issue number5
Publication statusPublished - 2000

Fingerprint

pathogenesis-related proteins
phenylalanine ammonia-lyase
Pseudomonas fluorescens
peanuts
proteins
leaf spot
leaves
rust diseases
plant growth-promoting rhizobacteria
extracellular fluids
leaf rust
foliar application
Arachis hypogaea
petioles
chitinase
enzyme activity
greenhouses
stems

Keywords

  • β-1,3-glucanase
  • Chitinase
  • Groundnut
  • Phenolics
  • Phenylalanine ammonia-lyase
  • Pseudomonas fluorescens
  • Thaumatin-like protein

ASJC Scopus subject areas

  • Plant Science

Cite this

Induction of pathogenesis-related proteins, phenolics and phenylalanine ammonia-lyase in groundnut by Pseudomonas fluorescens. / Meena, B.; Radhajeyalakshmi, R.; Marimuthu, T.; Vidhyasekaran, P.; Doraiswamy, S.; Velazhahan, R.

In: Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, Vol. 107, No. 5, 2000, p. 514-527.

Research output: Contribution to journalArticle

Meena, B. ; Radhajeyalakshmi, R. ; Marimuthu, T. ; Vidhyasekaran, P. ; Doraiswamy, S. ; Velazhahan, R. / Induction of pathogenesis-related proteins, phenolics and phenylalanine ammonia-lyase in groundnut by Pseudomonas fluorescens. In: Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz. 2000 ; Vol. 107, No. 5. pp. 514-527.
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