Induction of pathogenesis-related proteins in sugarcane leaves and cell-cultures by a glycoprotein elicitor isolated from Colletotrichum falcatum

A. Ramesh Sundar, R. Velazhahan, S. Nagarathinam, P. Vidhyasekaran

Research output: Contribution to journalArticle

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Abstract

The induction of pathogenesis-related (PR) proteins in sugarcane (Saccharum officinarum L.) leaves and suspension-cultured cells in response to treatment with a glycoprotein elicitor isolated from Colletotrichum falcatum (the red rot pathogen) was investigated. Treatment of leaves and cells with the elicitor resulted in a much marked increase in the activities of chitinase and β-1,3-glucanase in red rot resistant (BO 91) than susceptible (CoC 671) sugarcane cultivar. SDS-PAGE analysis revealed that C. falcatum elicitor induced the accumulation of several proteins in suspension-cultured cells of resistant cultivar (BO 91); among them the 35 kDa protein was predominant. Whereas, a 27 kDa protein was induced predominantly in the cells of susceptible cultivar upon treatment with the elicitor. When sugarcane leaves were treated with C. falcatum elicitor, two proteins with apparent molecular masses of 25 and 27 kDa were induced both in the resistant and susceptible cultivars. However, the induction was stronger in the resistant than the susceptible cultivar. Immunoblot analysis for chitinase indicated that a protein with an apparent molecular mass of 37 kDa cross-reacting with barley chitinase antiserum was strongly induced in the suspension cultured cells of both the cultivars. The induction of 37 kDa chitinase was more in the cells of resistant cultivar than in the susceptible cultivar. Western blot analysis revealed that a 25 kDa thaumatin-like protein (TLP) cross-reacting with bean TLP antiserum was strongly induced in leaves and cultured cells of both resistant and susceptible cultivars due to elicitor treatment.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalBiologia Plantarum
Volume52
Issue number2
DOIs
Publication statusPublished - Jun 2008

Fingerprint

Glomerella tucumanensis
pathogenesis-related proteins
sugarcane
glycoproteins
cell culture
cultivars
chitinase
leaves
cultured cells
proteins
antiserum
molecular weight
elicitors
Saccharum officinarum
cells
beans
Western blotting
barley

Keywords

  • β-1,3-glucanase
  • Chitinase
  • Red rot pathogen
  • Saccharum officinarum
  • Thaumatin-like protein

ASJC Scopus subject areas

  • Plant Science
  • Horticulture

Cite this

Induction of pathogenesis-related proteins in sugarcane leaves and cell-cultures by a glycoprotein elicitor isolated from Colletotrichum falcatum. / Ramesh Sundar, A.; Velazhahan, R.; Nagarathinam, S.; Vidhyasekaran, P.

In: Biologia Plantarum, Vol. 52, No. 2, 06.2008, p. 321-328.

Research output: Contribution to journalArticle

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