Incidence of pantoea agglomerans associated with barnyard grass (Echinochloa crusgalli L.) in Pakistan

Sehrish Mushtaq*, Muhammad Shafiq, Ummad Hussain, Freeha Ali, Amna Ali, Faiza Khan, Muhammad Asim, Muhammad S. Haider

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Weeds are reservoirs for the survival of pathogenic microorganisms and cause of spread of infectious disease of plants through vectors. To minimize the spread of bacteria to other host crops, proper characterization is required to suggest suitable control strategy. Survey was conduct in December 2015, leaf blight was observed on barnyard grass growing in surroundings of citrus orchards located in Lahore, Pakistan. Isolation of the bacterium on nutrient agar medium was performed and their characterization based on morphological and biochemical tests. 16S rRNA was amplified directly with primers 27F/1492R, cloned and sequenced. Sequences were blast in NCBI data base which showed 99% identity to P. agglomerans strain. A neighbor-joining tree was generated with reported bacterial strain that were most closely to isolated strain P. agglomerans (LT592133). Pathogenicity test was conducted on barnyard grass with bacterial suspensions having 1.5 × 108 CFU/ml in controlled conditions to confirm Koch's postulates. According to results this is first report on the incidence of Pantoea agglomerans on Barnyard grass in Pakistan.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
JournalPakistan Journal of Phytopathology
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • 16S rRNA analysis
  • Barnyard grass
  • Pantoea agglomerans
  • Pathogenicity test

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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