TY - JOUR
T1 - In vitro tolerance to antifungal glycoalkaloids and biofilm forming ability of the antagonistic yeast Meyerozyma guilliermondii strain SQUCC-33Y
AU - Al-Maawali, Samiya Saleh
AU - Al-Sadi, Abdullah Mohammed
AU - Babu, S. P.Sathish
AU - Velazhahan, Rethinasamy
N1 - Funding Information:
This study was funded, in part, by Sultan Qaboos University and The Research Council, Sultanate of Oman grants CR/AGR/CROP/19/01 and RC/RG-AGR/CROP/19/02. The authors thank Dr. S. Muthukrishnan, Kansas State University, USA for critically reading the manuscript.
Publisher Copyright:
© 2021, Indian Phytopathological Society.
PY - 2021/2/8
Y1 - 2021/2/8
N2 - Meyerozyma guilliermondii strain SQUCC-33Y is an antagonistic yeast against Alternaria alternata, the tomato fruit rot pathogen. In a previous study, we demonstrated the potential of M. guilliermondii SQUCC-33Y in the control of A. alternata-induced postharvest fruit rot of tomato. In this study, M. guilliermondii SQUCC-33Y was evaluated for its ability to tolerate antifungal glycoalkaloids of tomato and to form biofilm under in vitro conditions. Glycoalkaloids were extracted from immature green tomato fruits. Electrospray ionization-mass spectrometry (ESI-MS) analysis showed the presence of α-tomatine, β-tomatine, tomatidine, lycoperoside A, lycoperoside H, coumaric acid and sinapic acid in the glycoalkaloids extract. In vitro antifungal assays showed that M. guilliermondii SQUCC-33Y and A. alternata were tolerant to glycoalkaloids of tomato, while Rhizoctonia solani was sensitive. M. guilliermondii SQUCC-33Y was able to form biofilm as determined by in vitro microtiter plate assay.
AB - Meyerozyma guilliermondii strain SQUCC-33Y is an antagonistic yeast against Alternaria alternata, the tomato fruit rot pathogen. In a previous study, we demonstrated the potential of M. guilliermondii SQUCC-33Y in the control of A. alternata-induced postharvest fruit rot of tomato. In this study, M. guilliermondii SQUCC-33Y was evaluated for its ability to tolerate antifungal glycoalkaloids of tomato and to form biofilm under in vitro conditions. Glycoalkaloids were extracted from immature green tomato fruits. Electrospray ionization-mass spectrometry (ESI-MS) analysis showed the presence of α-tomatine, β-tomatine, tomatidine, lycoperoside A, lycoperoside H, coumaric acid and sinapic acid in the glycoalkaloids extract. In vitro antifungal assays showed that M. guilliermondii SQUCC-33Y and A. alternata were tolerant to glycoalkaloids of tomato, while Rhizoctonia solani was sensitive. M. guilliermondii SQUCC-33Y was able to form biofilm as determined by in vitro microtiter plate assay.
KW - Alternaria alternata
KW - Biofilm formation
KW - Biological control
KW - Meyerozyma guilliermondii
KW - α-Tomatine
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U2 - 10.1007/s42360-020-00310-2
DO - 10.1007/s42360-020-00310-2
M3 - Article
AN - SCOPUS:85100604285
SN - 0367-973X
VL - 74
SP - 817
EP - 821
JO - Indian Phytopathology
JF - Indian Phytopathology
IS - 3
ER -