In vitro tolerance to antifungal glycoalkaloids and biofilm forming ability of the antagonistic yeast Meyerozyma guilliermondii strain SQUCC-33Y

Samiya Saleh Al-Maawali, Abdullah Mohammed Al-Sadi, S. P.Sathish Babu, Rethinasamy Velazhahan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Meyerozyma guilliermondii strain SQUCC-33Y is an antagonistic yeast against Alternaria alternata, the tomato fruit rot pathogen. In a previous study, we demonstrated the potential of M. guilliermondii SQUCC-33Y in the control of A. alternata-induced postharvest fruit rot of tomato. In this study, M. guilliermondii SQUCC-33Y was evaluated for its ability to tolerate antifungal glycoalkaloids of tomato and to form biofilm under in vitro conditions. Glycoalkaloids were extracted from immature green tomato fruits. Electrospray ionization-mass spectrometry (ESI-MS) analysis showed the presence of α-tomatine, β-tomatine, tomatidine, lycoperoside A, lycoperoside H, coumaric acid and sinapic acid in the glycoalkaloids extract. In vitro antifungal assays showed that M. guilliermondii SQUCC-33Y and A. alternata were tolerant to glycoalkaloids of tomato, while Rhizoctonia solani was sensitive. M. guilliermondii SQUCC-33Y was able to form biofilm as determined by in vitro microtiter plate assay.

Original languageEnglish
Pages (from-to)817-821
Number of pages5
JournalIndian Phytopathology
Volume74
Issue number3
DOIs
Publication statusPublished - Sep 2021

Keywords

  • Alternaria alternata
  • Biofilm formation
  • Biological control
  • Meyerozyma guilliermondii
  • α-Tomatine

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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