In vitro production of antifungal phenolic acids by Hypomyces perniciosus, the causal agent of wet bubble disease of Agaricus bisporus

Majida Mohammed Ali Al-Harrasi, Abdullah Mohammed Al-Sadi, Afrah Mohammed Al-Tamimi, Jamal Nasser Al-Sabahi, Rethinasamy Velazhahan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Wet bubble disease caused by Hypomyces perniciosus (syn: Mycogone perniciosa) is one of the major constraints in the production of button mushroom (Agaricus bisporus) worldwide. Deformation of basidiome and exudation of brown coloured liquid are the characteristic symptoms of wet bubble disease. The objective of this research was to assess the in vitro production of antimicrobial compounds by H. perniciosus. The culture filtrate of H. perniciosus induced leakage of cellular electrolytes from the mycelium of A. bisporus. Thin-layer chromatography analysis of the ethyl acetate extract of the culture filtrate of H. perniciosus revealed the presence of a predominant band at Rf 0.8 reacting to Folin–Ciocalteu reagent. The compounds at Rf 0.8 were capable of inducing electrolyte leakage from A. bisporus mycelium. Ultra-performance liquid chromatography analysis of the purified fraction revealed the presence of syringic, vanillic, cinnamic, 2-hydroxycinnamic, ferulic and p-coumaric acids suggesting that phenolic acids in the culture filtrate might be involved in the mycoparasitic activity of H. perniciosus.

Original languageEnglish
Pages (from-to)948-953
Number of pages6
JournalAll Life
Volume14
Issue number1
DOIs
Publication statusPublished - 2021

Keywords

  • Agaricus bisporus
  • Hypomyces perniciosus
  • antifungal
  • phenolic acids
  • wet bubble disease

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)

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