In vitro evaluation of solvent extracted compounds from edible macromycetes against phytopathogenic fungi

R. Radhajeyalakshmi, R. Velazhahan, V. Prakasam

Research output: Contribution to journalArticle

Abstract

Extraction of antimicrobial compounds from medicinal mushrooms, namely, Auricularia polytricha (Jew's ear), Lentinulla edodes (Shiitake) and Volvariella volvacea (paddy straw), has been done with different solvent systems (chloroform, ethyl acetate, ether and methanol) and tested against a wide range of phytopathogens by filter paper disk assay. All the three basidiomycetes inhibited the phytopathogens tested so far. This enumeration was based on the number of organisms inhibited and the diameter of inhibitory zones produced. From the results obtained, it could be observed that ethyl acetate was the best solvent for extracting antimicrobial substances from these medicinal mushrooms. Thin layer chromatography revealed the presence of several compounds with different Retention Factor (RF) values. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis profiling of V. volvacea showed the presence of several antimicrobial proteins with various molecular weights. Western blotting revealed the presence of thaumatin-like glycoproteins of molecular weight more than 45 kDa.

Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalArchives of Phytopathology and Plant Protection
Volume45
Issue number3
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Volvariella volvacea
plant pathogenic fungi
plant pathogens
Auricularia polytricha
anti-infective agents
Jews
molecular weight
antimicrobial proteins
Basidiomycota
thin layer chromatography
chloroform
paddies
polyacrylamide gel electrophoresis
glycoproteins
ethers
ears
straw
Western blotting
methanol
organisms

Keywords

  • Auricularia polytricha
  • basidiomycetes
  • Lentinulla edodes
  • phytopathogens
  • SDS-PAGE
  • TLC
  • Volvariella volvacea

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

In vitro evaluation of solvent extracted compounds from edible macromycetes against phytopathogenic fungi. / Radhajeyalakshmi, R.; Velazhahan, R.; Prakasam, V.

In: Archives of Phytopathology and Plant Protection, Vol. 45, No. 3, 02.2012, p. 293-300.

Research output: Contribution to journalArticle

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