Abstract
Extraction of antimicrobial compounds from medicinal mushrooms, namely, Auricularia polytricha (Jew's ear), Lentinulla edodes (Shiitake) and Volvariella volvacea (paddy straw), has been done with different solvent systems (chloroform, ethyl acetate, ether and methanol) and tested against a wide range of phytopathogens by filter paper disk assay. All the three basidiomycetes inhibited the phytopathogens tested so far. This enumeration was based on the number of organisms inhibited and the diameter of inhibitory zones produced. From the results obtained, it could be observed that ethyl acetate was the best solvent for extracting antimicrobial substances from these medicinal mushrooms. Thin layer chromatography revealed the presence of several compounds with different Retention Factor (RF) values. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis profiling of V. volvacea showed the presence of several antimicrobial proteins with various molecular weights. Western blotting revealed the presence of thaumatin-like glycoproteins of molecular weight more than 45 kDa.
| Original language | English |
|---|---|
| Pages (from-to) | 293-300 |
| Number of pages | 8 |
| Journal | Archives of Phytopathology and Plant Protection |
| Volume | 45 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Feb 2012 |
| Externally published | Yes |
Keywords
- Auricularia polytricha
- Lentinulla edodes
- SDS-PAGE
- TLC
- Volvariella volvacea
- basidiomycetes
- phytopathogens
ASJC Scopus subject areas
- Agronomy and Crop Science