In vitro apoptosis triggering in the BT-474 human breast cancer cell line by lyophilised camel's milk

Sidgi S A A Hasson, Juma Zaid Al-Busaidi, Zahra A M Al-Qarni, S. Rajapakse, Shadia Al-Bahlani, Mohamed Ahmed Idris, Talal A. Sallam

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Breast cancer is a global health concern and is a major cause of death among women. In Oman, it is the most common cancer in women, with an incidence rate of 15.6 per 100,000 Omani females. Various anticancer remedies have been discovered from natural products in the past and the search is continuing for additional examples. Cytotoxic natural compounds may have a major role in cancer therapy either in potentiating the effect of chemotherapy or reducing its harmful effects. Recently, a few studies have reported advantages of using crude camel milk in treating some forms of cancer. However, no adequate data are available on the lyophilised camel's milk responsibility for triggering apoptosis and oxidative stress associated with human breast cancer. The present study aimed to address the role of the lyophilised camel's milk in inducing proliferation repression of BT-474 and HEp-2 cells compared with the non-cancer HCC1937 BL cell line. Lyophilized camel's milk fundamentally repressed BT-474 cells growth and proliferation through the initiation of either the intrinsic and extrinsic apoptotic pathways as indicated by both caspase-3 mRNA and its action level, and induction of death receptors in BT-474 but not the HEp-2 cell line. In addition, lyophilised camel's milk enhanced the expression of oxidative stress markers, heme-oxygenase-1 and reactive oxygen species production in BT-474 cells. Increase in caspase-3 mRNA levels by the lyophilised camel's milk was completely prevented by the actinomycin D, a transcriptional inhibitor. This suggests that lyophilized camel's milk increased newly synthesized RNA. Interestingly,it significantly (p <0.003) repressed the growth of HEp-2 cells and BT-474 cells after treatment for 72 hours while 24 hours treatment repressed BT-474 cells alone. This finding suggests that the lyophilised camel's milk might instigate apoptosis through initiation of an alternative apoptotic pathway.

Original languageEnglish
Pages (from-to)6651-6661
Number of pages11
JournalAsian Pacific Journal of Cancer Prevention
Volume16
Issue number15
DOIs
Publication statusPublished - 2015

Fingerprint

Camelus
Milk
Apoptosis
Breast Neoplasms
Cell Line
Caspase 3
Oxidative Stress
Oman
Neoplasms
Death Domain Receptors
Messenger RNA
Heme Oxygenase-1
In Vitro Techniques
Dactinomycin
Growth
Biological Products
Cause of Death
Reactive Oxygen Species
Therapeutics
Cell Proliferation

Keywords

  • Anticancer activity
  • Breast cancer
  • Camel's milk
  • Oman
  • Zymography

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Public Health, Environmental and Occupational Health
  • Epidemiology

Cite this

In vitro apoptosis triggering in the BT-474 human breast cancer cell line by lyophilised camel's milk. / Hasson, Sidgi S A A; Al-Busaidi, Juma Zaid; Al-Qarni, Zahra A M; Rajapakse, S.; Al-Bahlani, Shadia; Idris, Mohamed Ahmed; Sallam, Talal A.

In: Asian Pacific Journal of Cancer Prevention, Vol. 16, No. 15, 2015, p. 6651-6661.

Research output: Contribution to journalArticle

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