In vitro antifungal activity of a 29-kDa glycoprotein purified from the galls of Quercus infectoria

K. Yamunarani, R. Jaganathan, R. Bhaskaran, P. Govindaraju, R. Velazhahan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The antifungal activity of 30 medicinal plants belonging to different families was tested in vitro on the phytopathogenic fungus Rhizoctonia solani. The results revealed that protein extract from the galls of Quercus infectoria belonging to Fagaceae family was highly effective in inhibiting the mycelial growth of R. solani. The gall extract of Q. infectoria also inhibited the growth of other agronomically important fungal pathogens viz. Fusarium oxysporum, Cochliobolus miyabeanus, Macrophomina phaseolina, Colletotrichum gloeosporioides, Magnaporthe salvinii, Cochliobolus lunatus, Alternaria solani, Pythium aphanidermatum and Colletotrichum falcatum. A 29-kDa glycoprotein was purified from the galls of Q. infectoria by ammonium sulphate fractionation followed by gel filtration on Sephadex G-50 column. The purified protein showed the absorption maxima at 640 nm and 308 nm. The purified protein was stable even after heating at 100 °C for 10 min or autoclaving at 121 °C for 20 min. When the 29-kDa protein was treated with NaIO4 and pronase the antifungal activity was drastically reduced. The 29-kDa protein inhibited the mycelial growth of R. solani and C. miyabeanus at 2 μg level.

Original languageEnglish
Pages (from-to)43-54
Number of pages12
JournalActa Phytopathologica et Entomologica Hungarica
Volume40
Issue number1-2
DOIs
Publication statusPublished - 2005

Fingerprint

Quercus infectoria
galls
glycoproteins
Thanatephorus cucumeris
proteins
Magnaporthe salvinii
Glomerella tucumanensis
Pythium aphanidermatum
Alternaria solani
Macrophomina phaseolina
Fagaceae
autoclaving
Glomerella cingulata
extracts
plant pathogenic fungi
Fusarium oxysporum
ammonium sulfate
medicinal plants
fractionation
gels

Keywords

  • Antifungal protein
  • Protein purification
  • Quercus infectoria

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

In vitro antifungal activity of a 29-kDa glycoprotein purified from the galls of Quercus infectoria. / Yamunarani, K.; Jaganathan, R.; Bhaskaran, R.; Govindaraju, P.; Velazhahan, R.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 40, No. 1-2, 2005, p. 43-54.

Research output: Contribution to journalArticle

Yamunarani, K. ; Jaganathan, R. ; Bhaskaran, R. ; Govindaraju, P. ; Velazhahan, R. / In vitro antifungal activity of a 29-kDa glycoprotein purified from the galls of Quercus infectoria. In: Acta Phytopathologica et Entomologica Hungarica. 2005 ; Vol. 40, No. 1-2. pp. 43-54.
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