In situ hybridization and the polymerase chain reaction (PCR) in the analysis of biopsies and exfoliative cytology specimens for definitve diagnosis of oral hairy leukoplakia (DHL)

M. J.E.M.F. Mabruk, S. R. Flint, M. Toner, I. Balluz, D. Coleman, D. Sullivan, G. J. Atkins

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Abstract

The definitive diagnosis of oral hairy leukoplakia (OHL) demands that Epstein‐Barr virus (EBV) is demonstrated in the lesional tissue, since the histopathological features on conventional light microscopy are not pathognomonic. We have investigated the possible use of polymerase chain reaction (PCR) technology in reaching a definitive diagnosis of this lesion by its application to ten biopsy specimens with definitive diagnoses of OHL determined by in situ hybridization. EBV DNA was demonstrated by PCR in all ten OHL biopsy specimens analysed, and none of ten control specimens. Furthermore, we have investigated the role of PCR in analysis of exfoliative cytology samples collected from the lateral border of the tongue by a minimally‐invasive scraping technique. EBV DNA was not only detected in all OHL lesional scrapings but also in more than one‐third of healthy controls, due to viral presence in saliva at the time of sampling. In this application, the highly sensitive PCR technique has low specificity and cannot be recommended.

Original languageEnglish
Pages (from-to)302-308
Number of pages7
JournalJournal of Oral Pathology & Medicine
Volume23
Issue number7
DOIs
Publication statusPublished - 1994

Fingerprint

Hairy Leukoplakia
In Situ Hybridization
Cell Biology
Biopsy
Polymerase Chain Reaction
DNA Viruses
Saliva
Tongue
Microscopy
Viruses
Technology
Light

Keywords

  • Epstein‐Barr virus
  • hairy leukoplakia
  • in situ hybridization
  • polymerase chain reaction
  • saliva

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Cite this

In situ hybridization and the polymerase chain reaction (PCR) in the analysis of biopsies and exfoliative cytology specimens for definitve diagnosis of oral hairy leukoplakia (DHL). / Mabruk, M. J.E.M.F.; Flint, S. R.; Toner, M.; Balluz, I.; Coleman, D.; Sullivan, D.; Atkins, G. J.

In: Journal of Oral Pathology & Medicine, Vol. 23, No. 7, 1994, p. 302-308.

Research output: Contribution to journalArticle

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