In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts

Muhammad Shafiq, Qiuying Zhang, Dengke Zhi, Kai Wang, Deling Kong, Dong Hwee Kim, Soo Hyun Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective - The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results - Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. Conclusions - SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.

Original languageEnglish
Pages (from-to)E117-E134
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume38
Issue number7
DOIs
Publication statusPublished - Jul 1 2018

Fingerprint

Chemokine CXCL12
Substance P
Blood Vessels
Regeneration
Transplants
Peptides
Collagen
Macrophages
Neointima
Abdominal Aorta
von Willebrand Factor
Mesenchymal Stromal Cells
Electron Scanning Microscopy

Keywords

  • endothelial cells
  • regeneration
  • stem cells
  • substance P
  • tissue engineering

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts. / Shafiq, Muhammad; Zhang, Qiuying; Zhi, Dengke; Wang, Kai; Kong, Deling; Kim, Dong Hwee; Kim, Soo Hyun.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 38, No. 7, 01.07.2018, p. E117-E134.

Research output: Contribution to journalArticle

Shafiq, Muhammad ; Zhang, Qiuying ; Zhi, Dengke ; Wang, Kai ; Kong, Deling ; Kim, Dong Hwee ; Kim, Soo Hyun. / In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2018 ; Vol. 38, No. 7. pp. E117-E134.
@article{c3927697d7d74252ba7dd2ed333f1ae0,
title = "In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts",
abstract = "Objective - The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results - Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. Conclusions - SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.",
keywords = "endothelial cells, regeneration, stem cells, substance P, tissue engineering",
author = "Muhammad Shafiq and Qiuying Zhang and Dengke Zhi and Kai Wang and Deling Kong and Kim, {Dong Hwee} and Kim, {Soo Hyun}",
year = "2018",
month = "7",
day = "1",
doi = "10.1161/ATVBAHA.118.310934",
language = "English",
volume = "38",
pages = "E117--E134",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - In Situ Blood Vessel Regeneration Using SP (Substance P) and SDF (Stromal Cell-Derived Factor)-1α Peptide Eluting Vascular Grafts

AU - Shafiq, Muhammad

AU - Zhang, Qiuying

AU - Zhi, Dengke

AU - Wang, Kai

AU - Kong, Deling

AU - Kim, Dong Hwee

AU - Kim, Soo Hyun

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Objective - The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results - Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. Conclusions - SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.

AB - Objective - The objective of this study was to develop small-diameter vascular grafts capable of eluting SDF (stromal cell-derived factor)-1α-derived peptide and SP (substance P) for in situ vascular regeneration. Approach and Results - Polycaprolactone (PCL)/collagen grafts containing SP or SDF-1α-derived peptide were fabricated by electrospinning. SP and SDF-1α peptide-loaded grafts recruited significantly higher numbers of mesenchymal stem cells than that of the control group. The in vivo potential of PCL/collagen, SDF-1, and SP grafts was assessed by implanting them in a rat abdominal aorta for up to 4 weeks. All grafts remained patent as observed using color Doppler and stereomicroscope. Host cells infiltrated into the graft wall and the neointima was formed in peptides-eluting grafts. The lumen of the SP grafts was covered by the endothelial cells with cobblestone-like morphology, which were elongated in the direction of the blood flow, as discerned using scanning electron microscopy. Moreover, SDF-1α and SP grafts led to the formation of a confluent endothelium as evaluated using immunofluorescence staining with von Willebrand factor antibody. SP and SDF-1α grafts also promoted smooth muscle cell regeneration, endogenous stem cell recruitment, and blood vessel formation, which was the most prominent in the SP grafts. Evaluation of inflammatory response showed that 3 groups did not significantly differ in terms of the numbers of proinflammatory macrophages, whereas SP grafts showed significantly higher numbers of proremodeling macrophages than that of the control and SDF-1α grafts. Conclusions - SDF-1α and SP grafts can be potential candidates for in situ vascular regeneration and are worthy for future investigations.

KW - endothelial cells

KW - regeneration

KW - stem cells

KW - substance P

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85054811503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054811503&partnerID=8YFLogxK

U2 - 10.1161/ATVBAHA.118.310934

DO - 10.1161/ATVBAHA.118.310934

M3 - Article

VL - 38

SP - E117-E134

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 7

ER -