Immunophenotypic characterization of benign and malignant prostatic lesions

R. Lakhtakia, R. Bharadwaj, V. K. Kumar, P. Mandal, S. K. Nema

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Biopsy diagnosis is the gold standard for differentiating benign and malignant prostatic enlargements. This study was aimed at supplementing biopsy diagnosis with immunophenotypic characters of prostatic lesions. Methods: Twenty five cases each of nodular hyperplasia and adenocarcinoma prostate were compared for their morphologic appearances and immunophenotyping, by studying antibodies to prostate specific antigen (PSA), transglutaminase, chromogranin and high molecular weight keratin, proliferating cell nuclear antigen, cell death (apoptosis) and neovascularisation (CD 34). Results: Markers of differentiation (PSA and transglutaminase) aided recognition of higher-grade tumours. PSA negativity avoided metaplasia being overcalled as carcinoma. Loss of basal cells around malignant prostatic acini as determined by high molecular weight keratin (HMWK), was useful in foci of atypical small acinar proliferation and in prostatic intraepithelial neoplasia. Assessment of proliferation indices identified subsets of tumours, within conventional morphologic Gleason's grades, with a higher growth fraction. Cell death determination and study of tumour vessels did not offer any improvement on morphology. Conclusion: Immunophenotypic assessment helps in refining morphologic diagnosis of prostatic lesions. Differentiation and proliferation markers objectively assess tumour characteristics with their biologic growth potential and are recommended for diagnostic use.They also help in assessement of response to therapy.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalMedical Journal Armed Forces India
Volume63
Issue number3
Publication statusPublished - Jul 2007

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Prostate-Specific Antigen
Transglutaminases
Differentiation Antigens
Keratins
Neoplasms
Cell Death
Molecular Weight
Prostatic Intraepithelial Neoplasia
Chromogranins
Biopsy
Immunophenotyping
Proliferating Cell Nuclear Antigen
Metaplasia
Growth
Hyperplasia
Prostate
Adenocarcinoma
Apoptosis
Carcinoma
Antibodies

Keywords

  • Benign
  • Immunophenotyping
  • Malignant
  • Prostate

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Lakhtakia, R., Bharadwaj, R., Kumar, V. K., Mandal, P., & Nema, S. K. (2007). Immunophenotypic characterization of benign and malignant prostatic lesions. Medical Journal Armed Forces India, 63(3), 243-248.

Immunophenotypic characterization of benign and malignant prostatic lesions. / Lakhtakia, R.; Bharadwaj, R.; Kumar, V. K.; Mandal, P.; Nema, S. K.

In: Medical Journal Armed Forces India, Vol. 63, No. 3, 07.2007, p. 243-248.

Research output: Contribution to journalArticle

Lakhtakia, R, Bharadwaj, R, Kumar, VK, Mandal, P & Nema, SK 2007, 'Immunophenotypic characterization of benign and malignant prostatic lesions', Medical Journal Armed Forces India, vol. 63, no. 3, pp. 243-248.
Lakhtakia R, Bharadwaj R, Kumar VK, Mandal P, Nema SK. Immunophenotypic characterization of benign and malignant prostatic lesions. Medical Journal Armed Forces India. 2007 Jul;63(3):243-248.
Lakhtakia, R. ; Bharadwaj, R. ; Kumar, V. K. ; Mandal, P. ; Nema, S. K. / Immunophenotypic characterization of benign and malignant prostatic lesions. In: Medical Journal Armed Forces India. 2007 ; Vol. 63, No. 3. pp. 243-248.
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