Immunohistological Insight into the Correlation between Neuropilin-1 and Epithelial-Mesenchymal Transition Markers in Epithelial Ovarian Cancer

Sirin A I Adham, Ibtisam Al Harrasi, Ibrahim Al Haddabi, Afrah Al Rashdi, Shadia Al Sinawi, Abdullah Al Maniri, Taher Ba-Omar, Brenda L. Coomber

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The mechanism by which neuropilin-1 (NRP-1) induces malignancy in Epithelial Ovarian Cancer (EOC) is still unknown. This study is the first to demonstrate the relationship between NRP-1 expression and EMT markers vimentin, N-cadherin, E-cadherin and Slug. We used tissue microarrays containing the three main subtypes of EOC tumors: serous, mucinous cystadenocarcinoma and endometrioid adenocarcinoma and representative cases retrieved from our pathology archives. Immunohistochemistry was performed to detect the expression levels and location of NRP-1 and the aforementioned EMT proteins. NRP-1 was mainly expressed on cancer cells but not in normal ovarian surface epithelium (OSE). The Immunoreactive Scoring (IRS) values revealed that the expression of NRP-1, Slug and E-cadherin in the malignant subtypes of ovarian tissues was significantly higher (5.18 ± 0.64, 4.84 ± 0.7, 4.98 ± 0.68, respectively) than their expression in the normal and benign tissues (1.04 ± 0.29, 0.84 ± 0.68, 1.71 ± 0.66, respectively), with no significant differences among the studied subtypes. Vimentin was expressed in the cancer cell component of 43% of tumors and it was exclusively localized in the stroma of all mucinous tumors. The Spearman’s rho value indicated that NRP-1 is positively related to the EMT markers E-cadherin and Slug. This notion might indicate that NRP-1 is a partner in the EMT process in EOC tumors.

Original languageEnglish
Pages (from-to)619-631
Number of pages13
JournalJournal of Histochemistry and Cytochemistry
Volume62
Issue number9
DOIs
Publication statusPublished - Sep 27 2014

Fingerprint

Neuropilin-1
Epithelial-Mesenchymal Transition
Cadherins
Gastropoda
Neoplasms
Vimentin
Serous Cystadenocarcinoma
Mucinous Cystadenocarcinoma
Endometrioid Carcinoma
Mucinous Adenocarcinoma
Cellular Structures
Ovarian epithelial cancer
Epithelium
Immunohistochemistry
Pathology

Keywords

  • biomarker
  • E-cadherin
  • EMT
  • neuropilin-1
  • OSE
  • slug
  • vimentin

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Medicine(all)

Cite this

Immunohistological Insight into the Correlation between Neuropilin-1 and Epithelial-Mesenchymal Transition Markers in Epithelial Ovarian Cancer. / Adham, Sirin A I; Al Harrasi, Ibtisam; Al Haddabi, Ibrahim; Al Rashdi, Afrah; Al Sinawi, Shadia; Al Maniri, Abdullah; Ba-Omar, Taher; Coomber, Brenda L.

In: Journal of Histochemistry and Cytochemistry, Vol. 62, No. 9, 27.09.2014, p. 619-631.

Research output: Contribution to journalArticle

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AU - Al Haddabi, Ibrahim

AU - Al Rashdi, Afrah

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AU - Al Maniri, Abdullah

AU - Ba-Omar, Taher

AU - Coomber, Brenda L.

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