Immunohistochemical evaluation of conjunctival fibrillin-1 in Marfan syndrome

Anuradha Ganesh, Charles Smith, Wilson Chan, Sheila Unger, Nada Quercia, Maurice Godfrey, Stephen Kraft, Raymond Buncic, Alex Levin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: To evaluate status of conjunctival fibrillin-1 in patients with Marfan syndrome with ectopia lentis. Methods: Frozen sections of conjunctiva from 6 patients with Marfan syndrome with ectopia lentis and from 15 age-matched control subjects were stained with mouse antihuman fibrillin-1 antibody, using an avidin biotin immunoperoxidase technique. The fibrillin-1 staining characteristics of conjunctiva were analyzed with the light microscope. Results: All the fresh frozen sections of conjunctival samples from control subjects demonstrated a characteristic pattern of fibrillin-1 staining. We observed a woven network of thin fibrils of uniform thickness surrounding collagen bundles. The fresh frozen samples from patients with Marfan syndrome showed consistent qualitative differences in fibrillin-1 staining when compared with samples from control subjects. The fibrils were longer and straighter than normal, varied in caliber, and showed fewer tendencies to form a woven pattern. Conclusions: Consistent, qualitative abnormalities in fibrillin-1 staining pattern can be seen in the conjunctiva of patients with Marfan syndrome with ectopia lentis. Conjunctival biopsy deserves further investigation as a diagnostic modality for Marfan syndrome in patients with ectopia lentis.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalArchives of Ophthalmology
Volume124
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Marfan Syndrome
Ectopia Lentis
Conjunctiva
Staining and Labeling
Frozen Sections
Avidin
Biotin
Immunoenzyme Techniques
Fibrillin-1
Collagen
Biopsy
Light
Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Ganesh, A., Smith, C., Chan, W., Unger, S., Quercia, N., Godfrey, M., ... Levin, A. (2006). Immunohistochemical evaluation of conjunctival fibrillin-1 in Marfan syndrome. Archives of Ophthalmology, 124(2), 205-209. https://doi.org/10.1001/archopht.124.2.205

Immunohistochemical evaluation of conjunctival fibrillin-1 in Marfan syndrome. / Ganesh, Anuradha; Smith, Charles; Chan, Wilson; Unger, Sheila; Quercia, Nada; Godfrey, Maurice; Kraft, Stephen; Buncic, Raymond; Levin, Alex.

In: Archives of Ophthalmology, Vol. 124, No. 2, 02.2006, p. 205-209.

Research output: Contribution to journalArticle

Ganesh, A, Smith, C, Chan, W, Unger, S, Quercia, N, Godfrey, M, Kraft, S, Buncic, R & Levin, A 2006, 'Immunohistochemical evaluation of conjunctival fibrillin-1 in Marfan syndrome', Archives of Ophthalmology, vol. 124, no. 2, pp. 205-209. https://doi.org/10.1001/archopht.124.2.205
Ganesh, Anuradha ; Smith, Charles ; Chan, Wilson ; Unger, Sheila ; Quercia, Nada ; Godfrey, Maurice ; Kraft, Stephen ; Buncic, Raymond ; Levin, Alex. / Immunohistochemical evaluation of conjunctival fibrillin-1 in Marfan syndrome. In: Archives of Ophthalmology. 2006 ; Vol. 124, No. 2. pp. 205-209.
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