Immunohistochemical determination of the P15INK4b protein expression in cutaneous squamous cell carcinoma

Ahmed I. Moad, Tan Mei Lan, Gurjeet Kaur, Hasnah Hashim, Mohamed J E M F Mabruk

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Abstract

The tumor suppressor gene p15INK4b is a cyclin-dependent kinase inhibitor, in which its inactivation has been determined in primary tumors and in several tumor-derived cell lines. The precise role of p15INK4b protein expression in cutaneous squamous cell carcinoma (SCC) is currently not known. In a previous study, we have shown the frequent occurrence of allelic imbalance/loss of heterozygosity in cutaneous SCC using two microsatellite markers flanking the p15INK4b gene. This study is a continuation of our previous study and aims to determine the possible role of p15 INK4b protein expression in the genesis of cutaneous SCC. P15 INK4b protein expression was determined using immunohistochemical approach in 107 cases of cutaneous SCC tissue arrays and 19 cases of normal human skin tissues. The expression of p15INK4b was significantly reduced in the cutaneous SCC cases as compared with normal human skin (p = 0.017 and p < 0.05). However, there were no significant relationship between clinicopathologic variables of the patients (age, sex and tumor grade) and p15INK4b protein expression. The absence of p15INK4b expression in the majority of tissue microarray cores of cutaneous SCC indicated that p15INK4b could possibly be involved in the pathogenesis of cutaneous SCC.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalJournal of Cutaneous Pathology
Volume36
Issue number2
DOIs
Publication statusPublished - Feb 2009

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Cyclin-Dependent Kinase Inhibitor p15
Squamous Cell Carcinoma
Skin
Loss of Heterozygosity
Allelic Imbalance
Cyclin-Dependent Kinases
Tumor Cell Line
Tumor Suppressor Genes
Microsatellite Repeats
Neoplasms
Proteins

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine
  • Histology

Cite this

Immunohistochemical determination of the P15INK4b protein expression in cutaneous squamous cell carcinoma. / Moad, Ahmed I.; Lan, Tan Mei; Kaur, Gurjeet; Hashim, Hasnah; Mabruk, Mohamed J E M F.

In: Journal of Cutaneous Pathology, Vol. 36, No. 2, 02.2009, p. 183-189.

Research output: Contribution to journalArticle

Moad, Ahmed I. ; Lan, Tan Mei ; Kaur, Gurjeet ; Hashim, Hasnah ; Mabruk, Mohamed J E M F. / Immunohistochemical determination of the P15INK4b protein expression in cutaneous squamous cell carcinoma. In: Journal of Cutaneous Pathology. 2009 ; Vol. 36, No. 2. pp. 183-189.
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