Immunofluorometric assay of pepsinogen C and preliminary clinical applications

E. P. Diamandis*, S. Nadkarni, B. Bhaumik, A. Abdelrahman, D. N. Melegos, G. Borchert, M. H. Black, M. Alonso, A. Salas, J. R. De los Toyos, A. Sampedro, C. Lopez-Otin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

We developed mouse monoclonal antibodies (Abs) against pepsinogen C with highly purified antigen isolated from gastric mucosa. The Abs were used to construct a two-site sandwich-type assay for pepsinogen C with time-resolved fluorometry as a detection technique. The assay has a detection limit of 0.1 μg/L and is precise (within-run and day-to-day CVs <11%). We used this assay to measure pepsinogen C in seminal plasma, breast cyst fluid, amniotic fluid, male and female serum, serum from patients with prostate cancer, urine, breast tumor cytosolic extracts, breast milk, and cerebrospinal fluid. Highest pepsinogen C concentrations were in seminal plasma, followed by breast cyst fluid and amniotic fluid. We found no correlation between prostate-specific antigen concentrations and concentrations of pepsinogen C in serum of prostate cancer patients, and concluded that this marker is not useful for either diagnosing or monitoring prostatic carcinoma. The availability of a highly sensitive, reliable, and convenient method for quantifying pepsinogen C will allow investigations into the possible diagnostic value of this analyte in various clinical conditions, including benign breast diseases, breast cancer, fertility and pregnancy.

Original languageEnglish
Pages (from-to)1365-1371
Number of pages7
JournalClinical Chemistry
Volume43
Issue number8
Publication statusPublished - 1997

Keywords

  • Amniotic fluid
  • Androgen- regulated genes
  • Breast cancer
  • Prognostic markers
  • Proteases

ASJC Scopus subject areas

  • Clinical Biochemistry

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