Immunocytochemical evaluation of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 in breast cancer cell blocks and corresponding tissue blocks

A single institutional experience

Nasar Alwahaibi, Hajer Albadi, Noha Almasrouri, Shadia Alsinawi, Najat Aldairi

Research output: Contribution to journalArticle

Abstract

Background: Immunohistochemistry (IHC) is a routinely performed method to demonstrate estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) in surgical breast cancer specimens but not on cell block (CB) of fine-needle aspiration (FNA). The aims of this study were to evaluate the expression of ER, PR, and HER2 using immunocytochemistry (ICC) on CB and compare with the corresponding tissue blocks as gold standard as well as to compare with other similar studies. Materials and Methods: Forty-eight breast carcinoma CB specimens with their corresponding tissue blocks were identified. ICC on CB for ER, PR, and HER2 was performed and compared with tissue blocks. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value were measured for each receptor. The degree of agreement between CB and tissue blocks was calculated using Cohen's kappa (κ) test. Results: ER results showed 67.7% sensitivity, 94.1% specificity, 95.5% PPV, and a moderate agreement (κ =0.588). PR results showed 50% sensitivity, 90% specificity, 87.5% PPV, and a fair agreement (κ =0.368). HER2 results showed 58.3% sensitivity, 100% specificity, 100% PPV, and a moderate agreement (κ =0.539). Conclusion: The results of this study confirm the wide variations that occur between CB ICC and tissue block IHC in the detection of ER, PR, and HER2 in breast cancers. In comparison with other studies, we report a low sensitivity and high specificity rates for ER, PR, and HER2 in FNA CB. Further studies are recommended.

Original languageEnglish
Pages (from-to)160-164
Number of pages5
JournalJournal of Medical Sciences (Taiwan)
Volume38
Issue number4
DOIs
Publication statusPublished - Jan 1 2018

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Progesterone Receptors
Estrogen Receptors
Breast Neoplasms
Immunohistochemistry
Sensitivity and Specificity
Fine Needle Biopsy
human ERBB2 protein

Keywords

  • Breast cancer
  • cell block
  • estrogen receptor
  • human epidermal growth factor receptor 2
  • immunocytochemistry
  • progesterone receptor

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{8d15e80c21844e1a874218c9f34245be,
title = "Immunocytochemical evaluation of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 in breast cancer cell blocks and corresponding tissue blocks: A single institutional experience",
abstract = "Background: Immunohistochemistry (IHC) is a routinely performed method to demonstrate estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) in surgical breast cancer specimens but not on cell block (CB) of fine-needle aspiration (FNA). The aims of this study were to evaluate the expression of ER, PR, and HER2 using immunocytochemistry (ICC) on CB and compare with the corresponding tissue blocks as gold standard as well as to compare with other similar studies. Materials and Methods: Forty-eight breast carcinoma CB specimens with their corresponding tissue blocks were identified. ICC on CB for ER, PR, and HER2 was performed and compared with tissue blocks. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value were measured for each receptor. The degree of agreement between CB and tissue blocks was calculated using Cohen's kappa (κ) test. Results: ER results showed 67.7{\%} sensitivity, 94.1{\%} specificity, 95.5{\%} PPV, and a moderate agreement (κ =0.588). PR results showed 50{\%} sensitivity, 90{\%} specificity, 87.5{\%} PPV, and a fair agreement (κ =0.368). HER2 results showed 58.3{\%} sensitivity, 100{\%} specificity, 100{\%} PPV, and a moderate agreement (κ =0.539). Conclusion: The results of this study confirm the wide variations that occur between CB ICC and tissue block IHC in the detection of ER, PR, and HER2 in breast cancers. In comparison with other studies, we report a low sensitivity and high specificity rates for ER, PR, and HER2 in FNA CB. Further studies are recommended.",
keywords = "Breast cancer, cell block, estrogen receptor, human epidermal growth factor receptor 2, immunocytochemistry, progesterone receptor",
author = "Nasar Alwahaibi and Hajer Albadi and Noha Almasrouri and Shadia Alsinawi and Najat Aldairi",
year = "2018",
month = "1",
day = "1",
doi = "10.4103/jmedsci.jmedsci_130_17",
language = "English",
volume = "38",
pages = "160--164",
journal = "Journal of Medical Sciences",
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publisher = "National Defense Medical Center",
number = "4",

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TY - JOUR

T1 - Immunocytochemical evaluation of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 in breast cancer cell blocks and corresponding tissue blocks

T2 - A single institutional experience

AU - Alwahaibi, Nasar

AU - Albadi, Hajer

AU - Almasrouri, Noha

AU - Alsinawi, Shadia

AU - Aldairi, Najat

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: Immunohistochemistry (IHC) is a routinely performed method to demonstrate estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) in surgical breast cancer specimens but not on cell block (CB) of fine-needle aspiration (FNA). The aims of this study were to evaluate the expression of ER, PR, and HER2 using immunocytochemistry (ICC) on CB and compare with the corresponding tissue blocks as gold standard as well as to compare with other similar studies. Materials and Methods: Forty-eight breast carcinoma CB specimens with their corresponding tissue blocks were identified. ICC on CB for ER, PR, and HER2 was performed and compared with tissue blocks. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value were measured for each receptor. The degree of agreement between CB and tissue blocks was calculated using Cohen's kappa (κ) test. Results: ER results showed 67.7% sensitivity, 94.1% specificity, 95.5% PPV, and a moderate agreement (κ =0.588). PR results showed 50% sensitivity, 90% specificity, 87.5% PPV, and a fair agreement (κ =0.368). HER2 results showed 58.3% sensitivity, 100% specificity, 100% PPV, and a moderate agreement (κ =0.539). Conclusion: The results of this study confirm the wide variations that occur between CB ICC and tissue block IHC in the detection of ER, PR, and HER2 in breast cancers. In comparison with other studies, we report a low sensitivity and high specificity rates for ER, PR, and HER2 in FNA CB. Further studies are recommended.

AB - Background: Immunohistochemistry (IHC) is a routinely performed method to demonstrate estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) in surgical breast cancer specimens but not on cell block (CB) of fine-needle aspiration (FNA). The aims of this study were to evaluate the expression of ER, PR, and HER2 using immunocytochemistry (ICC) on CB and compare with the corresponding tissue blocks as gold standard as well as to compare with other similar studies. Materials and Methods: Forty-eight breast carcinoma CB specimens with their corresponding tissue blocks were identified. ICC on CB for ER, PR, and HER2 was performed and compared with tissue blocks. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value were measured for each receptor. The degree of agreement between CB and tissue blocks was calculated using Cohen's kappa (κ) test. Results: ER results showed 67.7% sensitivity, 94.1% specificity, 95.5% PPV, and a moderate agreement (κ =0.588). PR results showed 50% sensitivity, 90% specificity, 87.5% PPV, and a fair agreement (κ =0.368). HER2 results showed 58.3% sensitivity, 100% specificity, 100% PPV, and a moderate agreement (κ =0.539). Conclusion: The results of this study confirm the wide variations that occur between CB ICC and tissue block IHC in the detection of ER, PR, and HER2 in breast cancers. In comparison with other studies, we report a low sensitivity and high specificity rates for ER, PR, and HER2 in FNA CB. Further studies are recommended.

KW - Breast cancer

KW - cell block

KW - estrogen receptor

KW - human epidermal growth factor receptor 2

KW - immunocytochemistry

KW - progesterone receptor

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U2 - 10.4103/jmedsci.jmedsci_130_17

DO - 10.4103/jmedsci.jmedsci_130_17

M3 - Article

VL - 38

SP - 160

EP - 164

JO - Journal of Medical Sciences

JF - Journal of Medical Sciences

SN - 1011-4564

IS - 4

ER -