Immune response deviation and enhanced expression of chemokine receptor CCR4 in TBI patients due to unknown serum factors

Dieter Cadosch, Mohamed S. Al-Mushaiqri, Oliver P. Gautschi, Erwin Chan, Florian J. Jung, Allan P. Skirving, Luis Filgueira

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Severe brain trauma leads to an activation of the immune system. To this date, neither the exact perturbation of the specific immune reaction induced by the traumatic brain injury (TBI), nor the interactions leading to the infiltration of peripheral immune cells into the brain are fully understood. Patients and methods: Serum was collected from 17 patients with TBI and a long bone fracture, 24 patients with an isolated long bone fracture and from healthy individuals. The effect of the serum on normal human monocytes and T-lymphocytes was tested in vitro by assessing proliferation and expression of surface markers, chemokine receptors and cytokines. Results: Serum collected from patients with a TBI and a long bone fracture increased the expression of the chemokine receptor CCR4 in monocytes when compared to patients with an isolated long bone fracture. Extending this comparison to T-lymphocytes, the serum from TBI patients induced lower proliferation rates and decreased expression of the pro-inflammatory cytokine TNF-α, while simultaneously increasing the secretion of immune-modulatory cytokines (IL-4, IL-10 and TGF-β) (p < 0.05). Conclusion: Patients with a TBI release currently unknown soluble factors into the circulating blood that up regulate expression of chemokine receptor CCR4 in peripheral blood monocytes whilst concurrently inducing expression of immunosuppressive cytokines by activated T-lymphocytes.

Original languageEnglish
JournalInjury
Volume41
Issue number6
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Chemokine Receptors
Bone Fractures
Serum
Cytokines
Monocytes
T-Lymphocytes
Immunosuppressive Agents
Traumatic Brain Injury
Interleukin-4
Interleukin-10
Immune System
Up-Regulation
Brain

Keywords

  • CCR4
  • Chemokines
  • Cytokines
  • Monocytes
  • T-lymphocytes
  • Traumatic brain injury

ASJC Scopus subject areas

  • Emergency Medicine
  • Orthopedics and Sports Medicine

Cite this

Immune response deviation and enhanced expression of chemokine receptor CCR4 in TBI patients due to unknown serum factors. / Cadosch, Dieter; Al-Mushaiqri, Mohamed S.; Gautschi, Oliver P.; Chan, Erwin; Jung, Florian J.; Skirving, Allan P.; Filgueira, Luis.

In: Injury, Vol. 41, No. 6, 06.2010.

Research output: Contribution to journalArticle

Cadosch, Dieter ; Al-Mushaiqri, Mohamed S. ; Gautschi, Oliver P. ; Chan, Erwin ; Jung, Florian J. ; Skirving, Allan P. ; Filgueira, Luis. / Immune response deviation and enhanced expression of chemokine receptor CCR4 in TBI patients due to unknown serum factors. In: Injury. 2010 ; Vol. 41, No. 6.
@article{5e7090d7caed4f5aab5b1426e7d6d4b9,
title = "Immune response deviation and enhanced expression of chemokine receptor CCR4 in TBI patients due to unknown serum factors",
abstract = "Background: Severe brain trauma leads to an activation of the immune system. To this date, neither the exact perturbation of the specific immune reaction induced by the traumatic brain injury (TBI), nor the interactions leading to the infiltration of peripheral immune cells into the brain are fully understood. Patients and methods: Serum was collected from 17 patients with TBI and a long bone fracture, 24 patients with an isolated long bone fracture and from healthy individuals. The effect of the serum on normal human monocytes and T-lymphocytes was tested in vitro by assessing proliferation and expression of surface markers, chemokine receptors and cytokines. Results: Serum collected from patients with a TBI and a long bone fracture increased the expression of the chemokine receptor CCR4 in monocytes when compared to patients with an isolated long bone fracture. Extending this comparison to T-lymphocytes, the serum from TBI patients induced lower proliferation rates and decreased expression of the pro-inflammatory cytokine TNF-α, while simultaneously increasing the secretion of immune-modulatory cytokines (IL-4, IL-10 and TGF-β) (p < 0.05). Conclusion: Patients with a TBI release currently unknown soluble factors into the circulating blood that up regulate expression of chemokine receptor CCR4 in peripheral blood monocytes whilst concurrently inducing expression of immunosuppressive cytokines by activated T-lymphocytes.",
keywords = "CCR4, Chemokines, Cytokines, Monocytes, T-lymphocytes, Traumatic brain injury",
author = "Dieter Cadosch and Al-Mushaiqri, {Mohamed S.} and Gautschi, {Oliver P.} and Erwin Chan and Jung, {Florian J.} and Skirving, {Allan P.} and Luis Filgueira",
year = "2010",
month = "6",
doi = "10.1016/j.injury.2009.09.001",
language = "English",
volume = "41",
journal = "Injury",
issn = "0020-1383",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Immune response deviation and enhanced expression of chemokine receptor CCR4 in TBI patients due to unknown serum factors

AU - Cadosch, Dieter

AU - Al-Mushaiqri, Mohamed S.

AU - Gautschi, Oliver P.

AU - Chan, Erwin

AU - Jung, Florian J.

AU - Skirving, Allan P.

AU - Filgueira, Luis

PY - 2010/6

Y1 - 2010/6

N2 - Background: Severe brain trauma leads to an activation of the immune system. To this date, neither the exact perturbation of the specific immune reaction induced by the traumatic brain injury (TBI), nor the interactions leading to the infiltration of peripheral immune cells into the brain are fully understood. Patients and methods: Serum was collected from 17 patients with TBI and a long bone fracture, 24 patients with an isolated long bone fracture and from healthy individuals. The effect of the serum on normal human monocytes and T-lymphocytes was tested in vitro by assessing proliferation and expression of surface markers, chemokine receptors and cytokines. Results: Serum collected from patients with a TBI and a long bone fracture increased the expression of the chemokine receptor CCR4 in monocytes when compared to patients with an isolated long bone fracture. Extending this comparison to T-lymphocytes, the serum from TBI patients induced lower proliferation rates and decreased expression of the pro-inflammatory cytokine TNF-α, while simultaneously increasing the secretion of immune-modulatory cytokines (IL-4, IL-10 and TGF-β) (p < 0.05). Conclusion: Patients with a TBI release currently unknown soluble factors into the circulating blood that up regulate expression of chemokine receptor CCR4 in peripheral blood monocytes whilst concurrently inducing expression of immunosuppressive cytokines by activated T-lymphocytes.

AB - Background: Severe brain trauma leads to an activation of the immune system. To this date, neither the exact perturbation of the specific immune reaction induced by the traumatic brain injury (TBI), nor the interactions leading to the infiltration of peripheral immune cells into the brain are fully understood. Patients and methods: Serum was collected from 17 patients with TBI and a long bone fracture, 24 patients with an isolated long bone fracture and from healthy individuals. The effect of the serum on normal human monocytes and T-lymphocytes was tested in vitro by assessing proliferation and expression of surface markers, chemokine receptors and cytokines. Results: Serum collected from patients with a TBI and a long bone fracture increased the expression of the chemokine receptor CCR4 in monocytes when compared to patients with an isolated long bone fracture. Extending this comparison to T-lymphocytes, the serum from TBI patients induced lower proliferation rates and decreased expression of the pro-inflammatory cytokine TNF-α, while simultaneously increasing the secretion of immune-modulatory cytokines (IL-4, IL-10 and TGF-β) (p < 0.05). Conclusion: Patients with a TBI release currently unknown soluble factors into the circulating blood that up regulate expression of chemokine receptor CCR4 in peripheral blood monocytes whilst concurrently inducing expression of immunosuppressive cytokines by activated T-lymphocytes.

KW - CCR4

KW - Chemokines

KW - Cytokines

KW - Monocytes

KW - T-lymphocytes

KW - Traumatic brain injury

UR - http://www.scopus.com/inward/record.url?scp=77952101144&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952101144&partnerID=8YFLogxK

U2 - 10.1016/j.injury.2009.09.001

DO - 10.1016/j.injury.2009.09.001

M3 - Article

VL - 41

JO - Injury

JF - Injury

SN - 0020-1383

IS - 6

ER -