Identification of quantitative trait loci for nitrogen use efficiency in maize

H. A.S. Agrama, A. G. Zakaria, F. B. Said, M. Tuinstra

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Intensively managed crop systems are normally dependent on nitrogen input to maximize yield potential. Improvements in nitrogen-use efficiency (NUE) in crop plants may support the development of cropping systems that are more economically efficient and environment friendly. The objective of this study was to map and characterize quantitative trait loci (QTL) for NUE in a maize population. In preliminary experiments, inbred lines contrasting for NUE were identified and were used to generate populations of F(2:3) families for genetic study. A total of 214 F(2:3) families were evaluated in replicated trials under high nitrogen (280 kg/ha) and low nitrogen (30 kg/ha) conditions in 1996 and 1997. Analysis of ear-leaf area, plant height, grain yield, ears per plant, kernels number per ear, and kernel weight indicated significant genetic variation among F(2:3) families. The heritability of these traits was found to be high (h2 = 0.57-0.81). The mapping population were genotyped using a set of 99 restriction fragment length polymorphism (RFLP) markers. A linkage map of these markers was developed and used to identify QTL. Between two and six loci were found to be associated with each trait. The correspondence of several genomic regions with traits measured under nitrogen limited conditions suggests the presence of QTL associated with NUE. QTLs will help breeders to improve their maize ideotype of a low-nitrogen efficiency by identifying those constitutive and adaptive traits involved in the expression of traits significantly correlated with yield, such as ear leaf area and number of ears per plant.

Original languageEnglish
Pages (from-to)187-195
Number of pages9
JournalMolecular Breeding
Volume5
Issue number2
DOIs
Publication statusPublished - 1999

Fingerprint

Quantitative Trait Loci
nutrient use efficiency
Zea mays
ears
quantitative trait loci
Nitrogen
corn
nitrogen
Ear
leaf area
ideotypes
crops
seeds
inbred lines
chromosome mapping
cropping systems
restriction fragment length polymorphism
heritability
Population
grain yield

Keywords

  • Gene mapping
  • Low nitrogen tolerance
  • Nitrogen use efficiency
  • RFLP
  • Zea mays L.

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Identification of quantitative trait loci for nitrogen use efficiency in maize. / Agrama, H. A.S.; Zakaria, A. G.; Said, F. B.; Tuinstra, M.

In: Molecular Breeding, Vol. 5, No. 2, 1999, p. 187-195.

Research output: Contribution to journalArticle

Agrama, H. A.S. ; Zakaria, A. G. ; Said, F. B. ; Tuinstra, M. / Identification of quantitative trait loci for nitrogen use efficiency in maize. In: Molecular Breeding. 1999 ; Vol. 5, No. 2. pp. 187-195.
@article{bbb0aa7826a0499b8e52ce51244f8bbb,
title = "Identification of quantitative trait loci for nitrogen use efficiency in maize",
abstract = "Intensively managed crop systems are normally dependent on nitrogen input to maximize yield potential. Improvements in nitrogen-use efficiency (NUE) in crop plants may support the development of cropping systems that are more economically efficient and environment friendly. The objective of this study was to map and characterize quantitative trait loci (QTL) for NUE in a maize population. In preliminary experiments, inbred lines contrasting for NUE were identified and were used to generate populations of F(2:3) families for genetic study. A total of 214 F(2:3) families were evaluated in replicated trials under high nitrogen (280 kg/ha) and low nitrogen (30 kg/ha) conditions in 1996 and 1997. Analysis of ear-leaf area, plant height, grain yield, ears per plant, kernels number per ear, and kernel weight indicated significant genetic variation among F(2:3) families. The heritability of these traits was found to be high (h2 = 0.57-0.81). The mapping population were genotyped using a set of 99 restriction fragment length polymorphism (RFLP) markers. A linkage map of these markers was developed and used to identify QTL. Between two and six loci were found to be associated with each trait. The correspondence of several genomic regions with traits measured under nitrogen limited conditions suggests the presence of QTL associated with NUE. QTLs will help breeders to improve their maize ideotype of a low-nitrogen efficiency by identifying those constitutive and adaptive traits involved in the expression of traits significantly correlated with yield, such as ear leaf area and number of ears per plant.",
keywords = "Gene mapping, Low nitrogen tolerance, Nitrogen use efficiency, RFLP, Zea mays L.",
author = "Agrama, {H. A.S.} and Zakaria, {A. G.} and Said, {F. B.} and M. Tuinstra",
year = "1999",
doi = "10.1023/A:1009669507144",
language = "English",
volume = "5",
pages = "187--195",
journal = "Molecular Breeding",
issn = "1380-3743",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Identification of quantitative trait loci for nitrogen use efficiency in maize

AU - Agrama, H. A.S.

AU - Zakaria, A. G.

AU - Said, F. B.

AU - Tuinstra, M.

PY - 1999

Y1 - 1999

N2 - Intensively managed crop systems are normally dependent on nitrogen input to maximize yield potential. Improvements in nitrogen-use efficiency (NUE) in crop plants may support the development of cropping systems that are more economically efficient and environment friendly. The objective of this study was to map and characterize quantitative trait loci (QTL) for NUE in a maize population. In preliminary experiments, inbred lines contrasting for NUE were identified and were used to generate populations of F(2:3) families for genetic study. A total of 214 F(2:3) families were evaluated in replicated trials under high nitrogen (280 kg/ha) and low nitrogen (30 kg/ha) conditions in 1996 and 1997. Analysis of ear-leaf area, plant height, grain yield, ears per plant, kernels number per ear, and kernel weight indicated significant genetic variation among F(2:3) families. The heritability of these traits was found to be high (h2 = 0.57-0.81). The mapping population were genotyped using a set of 99 restriction fragment length polymorphism (RFLP) markers. A linkage map of these markers was developed and used to identify QTL. Between two and six loci were found to be associated with each trait. The correspondence of several genomic regions with traits measured under nitrogen limited conditions suggests the presence of QTL associated with NUE. QTLs will help breeders to improve their maize ideotype of a low-nitrogen efficiency by identifying those constitutive and adaptive traits involved in the expression of traits significantly correlated with yield, such as ear leaf area and number of ears per plant.

AB - Intensively managed crop systems are normally dependent on nitrogen input to maximize yield potential. Improvements in nitrogen-use efficiency (NUE) in crop plants may support the development of cropping systems that are more economically efficient and environment friendly. The objective of this study was to map and characterize quantitative trait loci (QTL) for NUE in a maize population. In preliminary experiments, inbred lines contrasting for NUE were identified and were used to generate populations of F(2:3) families for genetic study. A total of 214 F(2:3) families were evaluated in replicated trials under high nitrogen (280 kg/ha) and low nitrogen (30 kg/ha) conditions in 1996 and 1997. Analysis of ear-leaf area, plant height, grain yield, ears per plant, kernels number per ear, and kernel weight indicated significant genetic variation among F(2:3) families. The heritability of these traits was found to be high (h2 = 0.57-0.81). The mapping population were genotyped using a set of 99 restriction fragment length polymorphism (RFLP) markers. A linkage map of these markers was developed and used to identify QTL. Between two and six loci were found to be associated with each trait. The correspondence of several genomic regions with traits measured under nitrogen limited conditions suggests the presence of QTL associated with NUE. QTLs will help breeders to improve their maize ideotype of a low-nitrogen efficiency by identifying those constitutive and adaptive traits involved in the expression of traits significantly correlated with yield, such as ear leaf area and number of ears per plant.

KW - Gene mapping

KW - Low nitrogen tolerance

KW - Nitrogen use efficiency

KW - RFLP

KW - Zea mays L.

UR - http://www.scopus.com/inward/record.url?scp=0033055161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033055161&partnerID=8YFLogxK

U2 - 10.1023/A:1009669507144

DO - 10.1023/A:1009669507144

M3 - Article

VL - 5

SP - 187

EP - 195

JO - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

IS - 2

ER -