Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley

L. S. Dahleen, H. A. Agrama, R. D. Horsley, B. J. Steffenson, P. B. Schwarz, A. Mesfin, J. D. Franckowiak

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Fusarium head blight (FHB) in barley and wheat, caused by Fusarium graminearum, is a continual problem worldwide. Primarily, FHB reduces yield and quality, and results in the production of the toxin deoxynivalenol (DON), which can affect food safety. Identification of QTLs for FHB severity, DON level and related traits heading-date (HD) and plant-height (HT) with consistent effects across a set of environments, would provide the basis for marker-assisted selection (MAS) and potentially increase the efficiency of selection for resistance. A segregating population of 75 double-haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB severity evaluation. A linkage map of 214 RFLP, SSR and AFLP markers was constructed. Phenotypic data were collected in replicated field trials from five environments in two growing seasons. The data were analyzed using MQTL software to detect quantitative trait locus (QTL) x environment (E) interactions. Because of the presence of QTL x E, the MQM procedure in MAPQTL was applied to identify QTLs in single environments. We identified nine QTLs for FHB severity and five for low DON. Many of the disease-related QTLs identified were coincident with FHB QTLs identified in previous studies. Only two of the QTLs identified in this study were consistent across all five environments, and both were Zhedar 2 specific. Five of the FHB QTLs were associated with HD, and two were associated with HT. Regions that appear to be promising candidates for MAS and further genetic analysis include the two FHB QTLs on chromosome 2H and one on 6H, which were also associated with low DON and later heading-date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance by MAS in barley to develop cultivars that will show effective resistance under disease pressure.

Original languageEnglish
Pages (from-to)95-104
Number of pages10
JournalTheoretical And Applied Genetics
Volume108
Issue number1
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Fusarium head blight
Fusarium
Hordeum
quantitative trait loci
barley
deoxynivalenol
heading
marker-assisted selection
Quantitative Trait Loci
chromosome mapping
Disease Resistance
Food Safety
Chromosome Mapping
Haploidy
Fusarium graminearum
Restriction Fragment Length Polymorphisms
Triticum
haploidy
amplified fragment length polymorphism
food safety

Keywords

  • Disease resistance
  • Gene mapping
  • Hordeum vulgare
  • Molecular markers

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Dahleen, L. S., Agrama, H. A., Horsley, R. D., Steffenson, B. J., Schwarz, P. B., Mesfin, A., & Franckowiak, J. D. (2003). Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley. Theoretical And Applied Genetics, 108(1), 95-104. https://doi.org/10.1007/s00122-003-1409-7

Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley. / Dahleen, L. S.; Agrama, H. A.; Horsley, R. D.; Steffenson, B. J.; Schwarz, P. B.; Mesfin, A.; Franckowiak, J. D.

In: Theoretical And Applied Genetics, Vol. 108, No. 1, 12.2003, p. 95-104.

Research output: Contribution to journalArticle

Dahleen, LS, Agrama, HA, Horsley, RD, Steffenson, BJ, Schwarz, PB, Mesfin, A & Franckowiak, JD 2003, 'Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley', Theoretical And Applied Genetics, vol. 108, no. 1, pp. 95-104. https://doi.org/10.1007/s00122-003-1409-7
Dahleen, L. S. ; Agrama, H. A. ; Horsley, R. D. ; Steffenson, B. J. ; Schwarz, P. B. ; Mesfin, A. ; Franckowiak, J. D. / Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley. In: Theoretical And Applied Genetics. 2003 ; Vol. 108, No. 1. pp. 95-104.
@article{39c078826db84d3f80d0cfbcb6bd8384,
title = "Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley",
abstract = "Fusarium head blight (FHB) in barley and wheat, caused by Fusarium graminearum, is a continual problem worldwide. Primarily, FHB reduces yield and quality, and results in the production of the toxin deoxynivalenol (DON), which can affect food safety. Identification of QTLs for FHB severity, DON level and related traits heading-date (HD) and plant-height (HT) with consistent effects across a set of environments, would provide the basis for marker-assisted selection (MAS) and potentially increase the efficiency of selection for resistance. A segregating population of 75 double-haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB severity evaluation. A linkage map of 214 RFLP, SSR and AFLP markers was constructed. Phenotypic data were collected in replicated field trials from five environments in two growing seasons. The data were analyzed using MQTL software to detect quantitative trait locus (QTL) x environment (E) interactions. Because of the presence of QTL x E, the MQM procedure in MAPQTL was applied to identify QTLs in single environments. We identified nine QTLs for FHB severity and five for low DON. Many of the disease-related QTLs identified were coincident with FHB QTLs identified in previous studies. Only two of the QTLs identified in this study were consistent across all five environments, and both were Zhedar 2 specific. Five of the FHB QTLs were associated with HD, and two were associated with HT. Regions that appear to be promising candidates for MAS and further genetic analysis include the two FHB QTLs on chromosome 2H and one on 6H, which were also associated with low DON and later heading-date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance by MAS in barley to develop cultivars that will show effective resistance under disease pressure.",
keywords = "Disease resistance, Gene mapping, Hordeum vulgare, Molecular markers",
author = "Dahleen, {L. S.} and Agrama, {H. A.} and Horsley, {R. D.} and Steffenson, {B. J.} and Schwarz, {P. B.} and A. Mesfin and Franckowiak, {J. D.}",
year = "2003",
month = "12",
doi = "10.1007/s00122-003-1409-7",
language = "English",
volume = "108",
pages = "95--104",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley

AU - Dahleen, L. S.

AU - Agrama, H. A.

AU - Horsley, R. D.

AU - Steffenson, B. J.

AU - Schwarz, P. B.

AU - Mesfin, A.

AU - Franckowiak, J. D.

PY - 2003/12

Y1 - 2003/12

N2 - Fusarium head blight (FHB) in barley and wheat, caused by Fusarium graminearum, is a continual problem worldwide. Primarily, FHB reduces yield and quality, and results in the production of the toxin deoxynivalenol (DON), which can affect food safety. Identification of QTLs for FHB severity, DON level and related traits heading-date (HD) and plant-height (HT) with consistent effects across a set of environments, would provide the basis for marker-assisted selection (MAS) and potentially increase the efficiency of selection for resistance. A segregating population of 75 double-haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB severity evaluation. A linkage map of 214 RFLP, SSR and AFLP markers was constructed. Phenotypic data were collected in replicated field trials from five environments in two growing seasons. The data were analyzed using MQTL software to detect quantitative trait locus (QTL) x environment (E) interactions. Because of the presence of QTL x E, the MQM procedure in MAPQTL was applied to identify QTLs in single environments. We identified nine QTLs for FHB severity and five for low DON. Many of the disease-related QTLs identified were coincident with FHB QTLs identified in previous studies. Only two of the QTLs identified in this study were consistent across all five environments, and both were Zhedar 2 specific. Five of the FHB QTLs were associated with HD, and two were associated with HT. Regions that appear to be promising candidates for MAS and further genetic analysis include the two FHB QTLs on chromosome 2H and one on 6H, which were also associated with low DON and later heading-date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance by MAS in barley to develop cultivars that will show effective resistance under disease pressure.

AB - Fusarium head blight (FHB) in barley and wheat, caused by Fusarium graminearum, is a continual problem worldwide. Primarily, FHB reduces yield and quality, and results in the production of the toxin deoxynivalenol (DON), which can affect food safety. Identification of QTLs for FHB severity, DON level and related traits heading-date (HD) and plant-height (HT) with consistent effects across a set of environments, would provide the basis for marker-assisted selection (MAS) and potentially increase the efficiency of selection for resistance. A segregating population of 75 double-haploid lines, developed from the three-way cross Zhedar 2/ND9712//Foster, was used for genome mapping and FHB severity evaluation. A linkage map of 214 RFLP, SSR and AFLP markers was constructed. Phenotypic data were collected in replicated field trials from five environments in two growing seasons. The data were analyzed using MQTL software to detect quantitative trait locus (QTL) x environment (E) interactions. Because of the presence of QTL x E, the MQM procedure in MAPQTL was applied to identify QTLs in single environments. We identified nine QTLs for FHB severity and five for low DON. Many of the disease-related QTLs identified were coincident with FHB QTLs identified in previous studies. Only two of the QTLs identified in this study were consistent across all five environments, and both were Zhedar 2 specific. Five of the FHB QTLs were associated with HD, and two were associated with HT. Regions that appear to be promising candidates for MAS and further genetic analysis include the two FHB QTLs on chromosome 2H and one on 6H, which were also associated with low DON and later heading-date in multiple environments. This study provides a starting point for manipulating Zhedar 2-derived resistance by MAS in barley to develop cultivars that will show effective resistance under disease pressure.

KW - Disease resistance

KW - Gene mapping

KW - Hordeum vulgare

KW - Molecular markers

UR - http://www.scopus.com/inward/record.url?scp=0346669741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346669741&partnerID=8YFLogxK

U2 - 10.1007/s00122-003-1409-7

DO - 10.1007/s00122-003-1409-7

M3 - Article

C2 - 14556050

AN - SCOPUS:0346669741

VL - 108

SP - 95

EP - 104

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 1

ER -