Identification of genome regions controlling cotyledon, pod wall/seed coat and pod wall resistance to pea weevil through QTL mapping

N. Aryamanesh, Y. Zeng, O. Byrne, D. C. Hardie, A. M. Al-Subhi, T. Khan, K. H. Siddique, G. Yan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pea weevil, Bruchus pisorum, is one of the limiting factors for field pea (Pisum sativum) cultivation in the world with pesticide application the only available method for its control. Resistance to pea weevil has been found in an accession of Pisum fulvum but transfer of this resistance to cultivated pea (P. sativum) is limited due to a lack of easy-to-use techniques for screening interspecific breeding populations. To address this problem, an interspecific population was created from a cross between cultivated field pea and P. fulvum (resistance source). Quantitative trait locus (QTL) mapping was performed to discover the regions associated with resistance to cotyledon, pod wall/seed coat and pod wall resistance. Three major QTLs, located on linkage groups LG2, LG4 and LG5 were found for cotyledon resistance explaining approximately 80 % of the phenotypic variation. Two major QTLs were found for pod wall/seed coat resistance on LG2 and LG5 explaining approximately 70 % of the phenotypic variation. Co-linearity of QTLs for cotyledon and pod wall/seed coat resistance suggested that the mechanism of resistance for these two traits might act through the same pathways. Only one QTL was found for pod wall resistance on LG7 explaining approximately 9 % of the phenotypic variation. This is the first report on the development of QTL markers to probe Pisum germplasm for pea weevil resistance genes. These flanking markers will be useful in accelerating the process of screening when breeding for pea weevil resistance.

Original languageEnglish
Pages (from-to)489-497
Number of pages9
JournalTheoretical And Applied Genetics
Volume127
Issue number2
DOIs
Publication statusPublished - Nov 15 2014

Fingerprint

Bruchus pisorum
Weevils
Cotyledon
Quantitative Trait Loci
Peas
pods
cotyledons
quantitative trait loci
Seeds
Genome
genome
phenotypic variation
peas
Pisum sativum
screening
Pisum
Breeding
breeding
resistance mechanisms
linkage groups

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Biotechnology

Cite this

Identification of genome regions controlling cotyledon, pod wall/seed coat and pod wall resistance to pea weevil through QTL mapping. / Aryamanesh, N.; Zeng, Y.; Byrne, O.; Hardie, D. C.; Al-Subhi, A. M.; Khan, T.; Siddique, K. H.; Yan, G.

In: Theoretical And Applied Genetics, Vol. 127, No. 2, 15.11.2014, p. 489-497.

Research output: Contribution to journalArticle

Aryamanesh, N. ; Zeng, Y. ; Byrne, O. ; Hardie, D. C. ; Al-Subhi, A. M. ; Khan, T. ; Siddique, K. H. ; Yan, G. / Identification of genome regions controlling cotyledon, pod wall/seed coat and pod wall resistance to pea weevil through QTL mapping. In: Theoretical And Applied Genetics. 2014 ; Vol. 127, No. 2. pp. 489-497.
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