Identification of Chilli leaf curl virus associated with tomato leaf curl betasatellite infecting Mentha in Oman

Muhammad Shahid, Amir Raza, Abdullah Al-Sadi, Rob W. Briddon

Research output: Contribution to journalComment/debate

Abstract

Mint (Mentha sp.; family: Lamiaceae) plants showing yellowing and stunting symptoms from Salalah, Dhofar region, Oman were shown to be infected by a monopartite begomovirus (family: Geminiviridae). Cloning and sequencing of restriction endonuclease digested rolling-circle amplified viral DNA identified a begomovirus associated with the symptomatic mint plants. Further analysis of complete sequences showed the virus to be a variant of the ‘Oman’ strain of Chili leaf curl virus (ChLCV-OM), a virus prevalent in a number of crops across Oman. However, the ChLCV-OM identified in mint has a recombination pattern distinct from all other ChLCV isolates. Additionally, PCR amplification and sequencing showed the mint plants to also harbour the betasatellite, Tomato leaf curl betasatellite (ToLCB). This is the first identification of ChLCV in association with ToLCB infecting mint. The distinct recombination pattern of ChLCV-OM from mint suggests that the host is epidemiologically important in the spread of ChLCV-OM to other crops in Oman.

Original languageEnglish
JournalCanadian Journal of Plant Pathology
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Mentha
leaf curling
mint
Oman
tomatoes
Begomovirus
Geminiviridae
viruses
restriction endonucleases
crops
Lamiaceae
growth retardation
signs and symptoms (plants)
Chilli leaf curl virus
molecular cloning
DNA

Keywords

  • begomovirus
  • betasatellite
  • Chili leaf curl virus
  • Mentha
  • tomato leaf curl betasatellite

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "Identification of Chilli leaf curl virus associated with tomato leaf curl betasatellite infecting Mentha in Oman",
abstract = "Mint (Mentha sp.; family: Lamiaceae) plants showing yellowing and stunting symptoms from Salalah, Dhofar region, Oman were shown to be infected by a monopartite begomovirus (family: Geminiviridae). Cloning and sequencing of restriction endonuclease digested rolling-circle amplified viral DNA identified a begomovirus associated with the symptomatic mint plants. Further analysis of complete sequences showed the virus to be a variant of the ‘Oman’ strain of Chili leaf curl virus (ChLCV-OM), a virus prevalent in a number of crops across Oman. However, the ChLCV-OM identified in mint has a recombination pattern distinct from all other ChLCV isolates. Additionally, PCR amplification and sequencing showed the mint plants to also harbour the betasatellite, Tomato leaf curl betasatellite (ToLCB). This is the first identification of ChLCV in association with ToLCB infecting mint. The distinct recombination pattern of ChLCV-OM from mint suggests that the host is epidemiologically important in the spread of ChLCV-OM to other crops in Oman.",
keywords = "begomovirus, betasatellite, Chili leaf curl virus, Mentha, tomato leaf curl betasatellite",
author = "Muhammad Shahid and Amir Raza and Abdullah Al-Sadi and Briddon, {Rob W.}",
year = "2019",
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T1 - Identification of Chilli leaf curl virus associated with tomato leaf curl betasatellite infecting Mentha in Oman

AU - Shahid, Muhammad

AU - Raza, Amir

AU - Al-Sadi, Abdullah

AU - Briddon, Rob W.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Mint (Mentha sp.; family: Lamiaceae) plants showing yellowing and stunting symptoms from Salalah, Dhofar region, Oman were shown to be infected by a monopartite begomovirus (family: Geminiviridae). Cloning and sequencing of restriction endonuclease digested rolling-circle amplified viral DNA identified a begomovirus associated with the symptomatic mint plants. Further analysis of complete sequences showed the virus to be a variant of the ‘Oman’ strain of Chili leaf curl virus (ChLCV-OM), a virus prevalent in a number of crops across Oman. However, the ChLCV-OM identified in mint has a recombination pattern distinct from all other ChLCV isolates. Additionally, PCR amplification and sequencing showed the mint plants to also harbour the betasatellite, Tomato leaf curl betasatellite (ToLCB). This is the first identification of ChLCV in association with ToLCB infecting mint. The distinct recombination pattern of ChLCV-OM from mint suggests that the host is epidemiologically important in the spread of ChLCV-OM to other crops in Oman.

AB - Mint (Mentha sp.; family: Lamiaceae) plants showing yellowing and stunting symptoms from Salalah, Dhofar region, Oman were shown to be infected by a monopartite begomovirus (family: Geminiviridae). Cloning and sequencing of restriction endonuclease digested rolling-circle amplified viral DNA identified a begomovirus associated with the symptomatic mint plants. Further analysis of complete sequences showed the virus to be a variant of the ‘Oman’ strain of Chili leaf curl virus (ChLCV-OM), a virus prevalent in a number of crops across Oman. However, the ChLCV-OM identified in mint has a recombination pattern distinct from all other ChLCV isolates. Additionally, PCR amplification and sequencing showed the mint plants to also harbour the betasatellite, Tomato leaf curl betasatellite (ToLCB). This is the first identification of ChLCV in association with ToLCB infecting mint. The distinct recombination pattern of ChLCV-OM from mint suggests that the host is epidemiologically important in the spread of ChLCV-OM to other crops in Oman.

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KW - betasatellite

KW - Chili leaf curl virus

KW - Mentha

KW - tomato leaf curl betasatellite

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