Identification and characterization of an Onchocerca volvulus cDNA clone encoding a microfilarial surface-associated antigen

Sara Lustigman, Betsy Brotman, Eugene H. Johnson, Andrea B. Smith, Tellervo Huima, Alfred M. Prince

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The identification and characterization of a recombinant cDNA clone (OV103) expressing a microfilarial surface-associated antigen of Onchocerca volvulus is described. OV103 was identified and isolated from a λgt11 cDNA expression library derived from adult O. volvulus mRNA using a chimpanzee antiserum, taken 2 years after infection with third-stage larvae of O. volvulus. The cDNA clone encodes a 12.5-kDa protein that corresponds to a 15-kDa parasite protein present in microfilariae and adult female worms. The antigen encoded by this clone is located in the basal layer of the cuticle and the hypodermis of the female adult worm, and on the surface of microfilariae. OV103 fusion polypeptide is recognized only by some sera from onchocerciasis infected subjects (57%), but more significantly (89%) by sera from individuals that have low levels of patent infection. In addition, the antibody response to this protein developed before appearance of microfilariae in the skin of chimpanzees that had developed non-patent or low level patent infections, while the antibody response in chimpanzees with high levels of microfilariae appeared later at the time of appearance of microfilariae. Preliminary experiments indicated that affinity purified antibodies directed against OV103 fusion polypeptide mediated killing of nodular microfilariae in vitro in the presence of normal peripheral blood granulocytes.

Original languageEnglish
Pages (from-to)79-93
Number of pages15
JournalMolecular and Biochemical Parasitology
Volume50
Issue number1
DOIs
Publication statusPublished - 1992

Fingerprint

Onchocerca volvulus
Microfilariae
Surface Antigens
Complementary DNA
Clone Cells
Pan troglodytes
Antibody Formation
Infection
Onchocerciasis
Peptides
Proteins
Antibody Affinity
Subcutaneous Tissue
Serum
Gene Library
Granulocytes
Larva
Immune Sera
Parasites
Antigens

Keywords

  • Immunoelectron microscopy
  • Killing of microfilariae
  • Microfilaria
  • Onchocerca volvulus
  • Recombinant antigen
  • Surface-associated antigen

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Identification and characterization of an Onchocerca volvulus cDNA clone encoding a microfilarial surface-associated antigen. / Lustigman, Sara; Brotman, Betsy; Johnson, Eugene H.; Smith, Andrea B.; Huima, Tellervo; Prince, Alfred M.

In: Molecular and Biochemical Parasitology, Vol. 50, No. 1, 1992, p. 79-93.

Research output: Contribution to journalArticle

Lustigman, Sara ; Brotman, Betsy ; Johnson, Eugene H. ; Smith, Andrea B. ; Huima, Tellervo ; Prince, Alfred M. / Identification and characterization of an Onchocerca volvulus cDNA clone encoding a microfilarial surface-associated antigen. In: Molecular and Biochemical Parasitology. 1992 ; Vol. 50, No. 1. pp. 79-93.
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