TY - JOUR
T1 - Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library
AU - Westgaard, Jon Ivar
AU - Tafese, Tekle
AU - Wesmajervi, Mette Serine
AU - Stenvik, Jørgen
AU - Fjalestad, Kjersti Turid
AU - Damsgård, Børge
AU - Delghandi, Madjid
N1 - Funding Information:
Acknowledgements The present study was supported by the National Programme for Research in Functional Genomics in Norway (FUGE) within the Research Council of Norway (no. 162486/S10), by the Research Council of Norway (no. 164981/ S40) and by the EU project SEAFOODplus (no. 506359). We thank Dr. Marie Cooper for reading of the manuscript and making helpful suggestions.
PY - 2007/6
Y1 - 2007/6
N2 - A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122-0.907 and 0.673-0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy-Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.
AB - A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122-0.907 and 0.673-0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy-Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.
KW - Atlantic cod
KW - Dinucleotide repeats
KW - Enriched genomic library
KW - Microsatellites
KW - Type II markers
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U2 - 10.1007/s10592-006-9200-3
DO - 10.1007/s10592-006-9200-3
M3 - Article
AN - SCOPUS:34247582225
VL - 8
SP - 749
EP - 751
JO - Conservation Genetics
JF - Conservation Genetics
SN - 1566-0621
IS - 3
ER -