Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library

Jon Ivar Westgaard, Tekle Tafese, Mette Serine Wesmajervi, Jørgen Stenvik, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122-0.907 and 0.673-0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy-Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.

Original languageEnglish
Pages (from-to)749-751
Number of pages3
JournalConservation Genetics
Volume8
Issue number3
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Gadus morhua
Microsatellite Repeats
Libraries
microsatellite repeats
loci
disequilibrium
heterozygosity
population genetics
allele
polymorphism
Linkage Disequilibrium
Population Genetics
ecology
Ecology
Alleles
linkage disequilibrium
chromosome mapping
Population
library
marker

Keywords

  • Atlantic cod
  • Dinucleotide repeats
  • Enriched genomic library
  • Microsatellites
  • Type II markers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Nature and Landscape Conservation
  • Genetics(clinical)

Cite this

Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. / Westgaard, Jon Ivar; Tafese, Tekle; Wesmajervi, Mette Serine; Stenvik, Jørgen; Fjalestad, Kjersti Turid; Damsgård, Børge; Delghandi, Madjid.

In: Conservation Genetics, Vol. 8, No. 3, 06.2007, p. 749-751.

Research output: Contribution to journalArticle

Westgaard, Jon Ivar ; Tafese, Tekle ; Wesmajervi, Mette Serine ; Stenvik, Jørgen ; Fjalestad, Kjersti Turid ; Damsgård, Børge ; Delghandi, Madjid. / Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. In: Conservation Genetics. 2007 ; Vol. 8, No. 3. pp. 749-751.
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