Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library

Jon Ivar Westgaard; Tekle Tafese; Mette Serine Wesmajervi; Jørgen Stenvik; Kjersti Turid Fjalestad; Børge Damsgård; Madjid Delghandi

doi:10.1007/s10592-006-9200-3

Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library

Jon Ivar Westgaard, Tekle Tafese, Mette Serine Wesmajervi, Jørgen Stenvik, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi^*

^*Corresponding author for this work

Excellence in Marine Biotechnology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122-0.907 and 0.673-0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy-Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.

Original language	English
Pages (from-to)	749-751
Number of pages	3
Journal	Conservation Genetics
Volume	8
Issue number	3
DOIs	https://doi.org/10.1007/s10592-006-9200-3
Publication status	Published - Jun 2007

Keywords

Atlantic cod
Dinucleotide repeats
Enriched genomic library
Microsatellites
Type II markers

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Genetics
Nature and Landscape Conservation
Genetics(clinical)

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Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. / Westgaard, Jon Ivar; Tafese, Tekle; Wesmajervi, Mette Serine; Stenvik, Jørgen; Fjalestad, Kjersti Turid; Damsgård, Børge; Delghandi, Madjid.

In: Conservation Genetics, Vol. 8, No. 3, 06.2007, p. 749-751.

Research output: Contribution to journal › Article › peer-review

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title = "Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library",

abstract = "A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122-0.907 and 0.673-0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy-Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.",

keywords = "Atlantic cod, Dinucleotide repeats, Enriched genomic library, Microsatellites, Type II markers",

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AU - Westgaard, Jon Ivar

AU - Tafese, Tekle

AU - Wesmajervi, Mette Serine

AU - Stenvik, Jørgen

AU - Fjalestad, Kjersti Turid

AU - Damsgård, Børge

AU - Delghandi, Madjid

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N2 - A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122-0.907 and 0.673-0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy-Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.

AB - A total of 13 polymorphic microsatellite markers were developed for Atlantic cod (Gadus morhua L.) from a repeat-enriched library. Polymorphism of each locus was assessed in 96 unrelated individuals from a natural population. The number of alleles per locus varied from 8 to 45. The ranges of observed and expected heterozygosity were 0.122-0.907 and 0.673-0.965, respectively. Four of the loci (Gmo-G24, Gmo-G40, Gmo-G46 and Gmo-G49) followed Hardy-Weinberg expectation. No evidence for linkage disequilibrium between pairs of loci was found in any combination of loci pairs, except between Gmo-G40 and Gmo-G43. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and for constructing linkage maps of Atlantic cod.

KW - Atlantic cod

KW - Dinucleotide repeats

KW - Enriched genomic library

KW - Microsatellites

KW - Type II markers

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Identification and characterisation of thirteen new microsatellites for Atlantic cod (Gadus morhua L.) from a repeat-enriched library

Abstract

Keywords

ASJC Scopus subject areas

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