Identification and characterisation of nine new gene-associated microsatellite markers for Atlantic cod (Gadus morhua L.)

Madjid Delghandi, Jørgen Stenvik, Frank Nilsen, Mette Serine Wesmajervi, Kjersti Turid Fjalestad, Børge Damsgård

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nine polymorphic microsatellite markers were identified by screening of 2464 ESTs derived from a cDNA library of Atlantic cod (Gadus morhua L.). About 35 novel microsatellite loci were selected and characterised in 96 individual cod. Nine markers were successfully amplified with number of alleles from 3 to 18 per locus and the average heterozygosity was 0.57 in the panel examined (range 0.29-0.86). All loci followed the Hardy-Weinberg expectation and no significant linkage disequilibrium was found in a test including all pairwise combinations. The gene identity was determined at four of the loci, confirming the associated microsatellites as Type I markers.

Original languageEnglish
Pages (from-to)747-749
Number of pages3
JournalConservation Genetics
Volume9
Issue number3
DOIs
Publication statusPublished - Jun 2008

Fingerprint

Gadus morhua
Microsatellite Repeats
microsatellite repeats
loci
gene
Genes
Gadiformes
genes
Expressed Sequence Tags
Linkage Disequilibrium
Gene Library
disequilibrium
heterozygosity
allele
cod (fish)
linkage disequilibrium
Alleles
cDNA libraries
screening
alleles

Keywords

  • Atlantic cod
  • cDNA
  • Expressed sequence tags (ESTs)
  • Gadus morhua
  • Microsatellites
  • Type I markers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)
  • Nature and Landscape Conservation

Cite this

Identification and characterisation of nine new gene-associated microsatellite markers for Atlantic cod (Gadus morhua L.). / Delghandi, Madjid; Stenvik, Jørgen; Nilsen, Frank; Wesmajervi, Mette Serine; Fjalestad, Kjersti Turid; Damsgård, Børge.

In: Conservation Genetics, Vol. 9, No. 3, 06.2008, p. 747-749.

Research output: Contribution to journalArticle

Delghandi, Madjid ; Stenvik, Jørgen ; Nilsen, Frank ; Wesmajervi, Mette Serine ; Fjalestad, Kjersti Turid ; Damsgård, Børge. / Identification and characterisation of nine new gene-associated microsatellite markers for Atlantic cod (Gadus morhua L.). In: Conservation Genetics. 2008 ; Vol. 9, No. 3. pp. 747-749.
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