Host-defense peptides from skin secretions of Fraser's clawed frog Xenopus fraseri (Pipidae): Further insight into the evolutionary history of the Xenopodinae

J. Michael Conlon, Milena Mechkarska, Jolanta Kolodziejek, Norbert Nowotny, Laurent Coquet, Jérôme Leprince, Thierry Jouenne, Hubert Vaudry

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Peptidomic analysis of norepinephrine-stimulated skin secretions of the tetraploid frog Xenopus fraseri Boulenger, 1905 (Pipidae) led to identification of 13 host-defense peptides. The primary structures of the peptides demonstrate that they belong to the magainin (3 peptides), peptide glycine-leucine-amide, PGLa (4 peptides), and xenopsin-precursor fragment, XPF (2 peptides) families, first identified in Xenopus laevis, together with caerulein precursor fragment-related peptides, CPF-RP (4 peptides), first identified in Silurana tropicalis. In addition, the secretions contain a molecular variant of xenopsin displaying the substitution Arg4 → Lys compared with X. laevis xenopsin and peptide glycine-tyrosine-amide (PGYa) (GRIIPIYPEFERVFA KKVYPLY.NH2) whose function is unknown. The most potent antimicrobial peptide identified is CPF-RP-F1 (GFGSVLGKALKFGANLL.NH2) with MIC = 12.5 μM against Staphylococcus aureus and 50 μM against Escherichia coli. On the basis of similarities in morphology and advertisement calls, X. fraseri has been placed in a species group that includes the octoploids Xenopus amieti and Xenopus andrei, and the tetraploid Xenopus pygmaeus. Cladistic analyses based upon the primary structures of magainin, PGLa, and CPF-RP peptides support a close evolutionary relationship between X. fraseri, X. amieti and X. andrei but suggest a more distant relationship with X. pygmaeus.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume12
DOIs
Publication statusPublished - 2014

Fingerprint

Pipidae
Xenopus
Anura
Skin
History
Peptides
xenopsin
Magainins
Tetraploidy
Xenopus laevis
Glycine
Ceruletide
Peptide Fragments
Leucine

Keywords

  • Caerulein-precursor fragment
  • Frog skin
  • Host-defense peptide
  • Magainin
  • PGLa

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology
  • Physiology
  • Medicine(all)

Cite this

Host-defense peptides from skin secretions of Fraser's clawed frog Xenopus fraseri (Pipidae) : Further insight into the evolutionary history of the Xenopodinae. / Conlon, J. Michael; Mechkarska, Milena; Kolodziejek, Jolanta; Nowotny, Norbert; Coquet, Laurent; Leprince, Jérôme; Jouenne, Thierry; Vaudry, Hubert.

In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 12, 2014, p. 45-52.

Research output: Contribution to journalArticle

Conlon, J. Michael ; Mechkarska, Milena ; Kolodziejek, Jolanta ; Nowotny, Norbert ; Coquet, Laurent ; Leprince, Jérôme ; Jouenne, Thierry ; Vaudry, Hubert. / Host-defense peptides from skin secretions of Fraser's clawed frog Xenopus fraseri (Pipidae) : Further insight into the evolutionary history of the Xenopodinae. In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 2014 ; Vol. 12. pp. 45-52.
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