Host-defense peptides from skin secretions of Fraser's clawed frog Xenopus fraseri (Pipidae)

Further insight into the evolutionary history of the Xenopodinae

J. Michael Conlon, Milena Mechkarska, Jolanta Kolodziejek, Norbert Nowotny, Laurent Coquet, Jérôme Leprince, Thierry Jouenne, Hubert Vaudry

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Peptidomic analysis of norepinephrine-stimulated skin secretions of the tetraploid frog Xenopus fraseri Boulenger, 1905 (Pipidae) led to identification of 13 host-defense peptides. The primary structures of the peptides demonstrate that they belong to the magainin (3 peptides), peptide glycine-leucine-amide, PGLa (4 peptides), and xenopsin-precursor fragment, XPF (2 peptides) families, first identified in Xenopus laevis, together with caerulein precursor fragment-related peptides, CPF-RP (4 peptides), first identified in Silurana tropicalis. In addition, the secretions contain a molecular variant of xenopsin displaying the substitution Arg4 → Lys compared with X. laevis xenopsin and peptide glycine-tyrosine-amide (PGYa) (GRIIPIYPEFERVFA KKVYPLY.NH2) whose function is unknown. The most potent antimicrobial peptide identified is CPF-RP-F1 (GFGSVLGKALKFGANLL.NH2) with MIC = 12.5 μM against Staphylococcus aureus and 50 μM against Escherichia coli. On the basis of similarities in morphology and advertisement calls, X. fraseri has been placed in a species group that includes the octoploids Xenopus amieti and Xenopus andrei, and the tetraploid Xenopus pygmaeus. Cladistic analyses based upon the primary structures of magainin, PGLa, and CPF-RP peptides support a close evolutionary relationship between X. fraseri, X. amieti and X. andrei but suggest a more distant relationship with X. pygmaeus.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume12
DOIs
Publication statusPublished - 2014

Fingerprint

Pipidae
Xenopus
Anura
Skin
History
Peptides
xenopsin
Magainins
Tetraploidy
Xenopus laevis
Glycine
Ceruletide
Peptide Fragments
Leucine

Keywords

  • Caerulein-precursor fragment
  • Frog skin
  • Host-defense peptide
  • Magainin
  • PGLa

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology
  • Physiology
  • Medicine(all)

Cite this

Host-defense peptides from skin secretions of Fraser's clawed frog Xenopus fraseri (Pipidae) : Further insight into the evolutionary history of the Xenopodinae. / Conlon, J. Michael; Mechkarska, Milena; Kolodziejek, Jolanta; Nowotny, Norbert; Coquet, Laurent; Leprince, Jérôme; Jouenne, Thierry; Vaudry, Hubert.

In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 12, 2014, p. 45-52.

Research output: Contribution to journalArticle

Conlon, J. Michael ; Mechkarska, Milena ; Kolodziejek, Jolanta ; Nowotny, Norbert ; Coquet, Laurent ; Leprince, Jérôme ; Jouenne, Thierry ; Vaudry, Hubert. / Host-defense peptides from skin secretions of Fraser's clawed frog Xenopus fraseri (Pipidae) : Further insight into the evolutionary history of the Xenopodinae. In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 2014 ; Vol. 12. pp. 45-52.
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