@article{150ab67fe693478eadd01a49a89be23e,
title = "HIV Gag p24 specific responses secreting IFN-γ and/or IL-2 in treatment-na{\"i}ve individuals in acute infection early disease (AIED) are associated with low viral load",
abstract = "HIV-specific immune responses in acute infection early disease (AIED) may be effective at controlling viral replication and in establishing viral load (VL) set point. However, evidence correlating the function and specificity of these responses with the VL set point is lacking. To address this issue, we screened cells from 59 treatment-na{\"i}ve HIV infected individuals (33 in AIED and 26 progressors) for responses to the entire HIV proteome using a dual color ELISPOT assay detecting 3 functional lymphocyte populations: single IFN-γ, dual IFN-γ/IL-2 and single IL-2 secreting cells. Responses characterized by dual secreting cells contributed more to the HIV specific response in AIED versus chronic infection. Of responses directed to individual HIV gene products the magnitude and breadth of only Gag p24-specific responses for the 3 functional subsets were associated with lower concurrent or set point VL. Therefore the early appearance of broader and more intense Gag-p24-specific responses may be a determinant of subsequent VL.",
keywords = "AIED, Dual color ELISPOT, HIV Gag p24 responses, HIV viremia",
author = "Ndongala, {Michel L.} and Yoav Peretz and Salix Boulet and Mehrnoosh Doroudchi and Bader Yassine-Diab and Boulassel, {Mohamed Rachid} and Danielle Rouleau and C{\'e}cile Tremblay and Roger LeBlanc and Routy, {Jean Pierre} and S{\'e}kaly, {Rafick Pierre} and Bernard, {Nicole F.}",
note = "Funding Information: We wish to acknowledge Ms. Chantal Grignon for nursing support for the leukapheresis infrastructure, Mr. Mario Legault, coordinator of the Montreal Primary Infection (PI) cohort. Ms. Nancy Simic, Marie-Pierre Boisvert and Mr. Saeid Sharafi for technical assistance and Dr. George Makedonas for guidance on analysis of flow cytometry data. We also thank Drs. John MacLoed, Marina Klein, Norbert Gilmore, Martin Potter, Michel Bissonnault, Marc-Andr{\'e} Charron, Annie Talbot and Bernard Lessard who recruited and followed participants included in this study. We are also grateful to participants in the Montreal PI cohort. This work was funded by a grant from the National Institutes of Health #AI043261-04 and the R{\'e}seau du SIDA et Maladies Infectieuses du Fonds de la Recherche en Sant{\'e} du Qu{\'e}bec (FRSQ). Y.P is the recipient of a scholarship from the FRSQ. J-P.R is the recipient of a Chercheur Boursier scholarship from the FRSQ. ",
year = "2009",
month = may,
doi = "10.1016/j.clim.2008.11.006",
language = "English",
volume = "131",
pages = "277--287",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "2",
}
