HIV Gag p24 specific responses secreting IFN-γ and/or IL-2 in treatment-naïve individuals in acute infection early disease (AIED) are associated with low viral load

Michel L. Ndongala, Yoav Peretz, Salix Boulet, Mehrnoosh Doroudchi, Bader Yassine-Diab, Mohamed Rachid Boulassel, Danielle Rouleau, Cécile Tremblay, Roger LeBlanc, Jean Pierre Routy, Rafick Pierre Sékaly, Nicole F. Bernard

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

HIV-specific immune responses in acute infection early disease (AIED) may be effective at controlling viral replication and in establishing viral load (VL) set point. However, evidence correlating the function and specificity of these responses with the VL set point is lacking. To address this issue, we screened cells from 59 treatment-naïve HIV infected individuals (33 in AIED and 26 progressors) for responses to the entire HIV proteome using a dual color ELISPOT assay detecting 3 functional lymphocyte populations: single IFN-γ, dual IFN-γ/IL-2 and single IL-2 secreting cells. Responses characterized by dual secreting cells contributed more to the HIV specific response in AIED versus chronic infection. Of responses directed to individual HIV gene products the magnitude and breadth of only Gag p24-specific responses for the 3 functional subsets were associated with lower concurrent or set point VL. Therefore the early appearance of broader and more intense Gag-p24-specific responses may be a determinant of subsequent VL.

Original languageEnglish
Pages (from-to)277-287
Number of pages11
JournalClinical Immunology
Volume131
Issue number2
DOIs
Publication statusPublished - May 2009

Keywords

  • AIED
  • Dual color ELISPOT
  • HIV Gag p24 responses
  • HIV viremia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Ndongala, M. L., Peretz, Y., Boulet, S., Doroudchi, M., Yassine-Diab, B., Boulassel, M. R., Rouleau, D., Tremblay, C., LeBlanc, R., Routy, J. P., Sékaly, R. P., & Bernard, N. F. (2009). HIV Gag p24 specific responses secreting IFN-γ and/or IL-2 in treatment-naïve individuals in acute infection early disease (AIED) are associated with low viral load. Clinical Immunology, 131(2), 277-287. https://doi.org/10.1016/j.clim.2008.11.006