High rates of denitrification and nitrous oxide emission in arid biological soil crusts from the Sultanate of Oman

Raeid M M Abed, Phyllis Lam, Dirk De Beer, Peter Stief

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Using a combination of process rate determination, microsensor profiling and molecular techniques, we demonstrated that denitrification, and not anaerobic ammonium oxidation (anammox), is the major nitrogen loss process in biological soil crusts from Oman. Potential denitrification rates were 584±101 and 58±20 μmol N m -2 h -1 for cyanobacterial and lichen crust, respectively. Complete denitrification to N 2 was further confirmed by an 15 NO 3 - tracer experiment with intact crust pieces that proceeded at rates of 103±19 and 27±8 μmol N m -2 h -1 for cyanobacterial and lichen crust, respectively. Strikingly, N 2 O gas was emitted at very high potential rates of 387±143 and 31±6 μmol N m -2 h -1 from the cyanobacterial and lichen crust, respectively, with N 2 O accounting for 53-66% of the total emission of nitrogenous gases. Microsensor measurements revealed that N 2 O was produced in the anoxic layer and thus apparently originated from incomplete denitrification. Using quantitative PCR, denitrification genes were detected in both the crusts and were expressed either in comparable (nirS) or slightly higher (narG) numbers in the cyanobacterial crusts. Although 99% of the nirS sequences in the cyanobacterial crust were affiliated to an uncultured denitrifying bacterium, 94% of these sequences were most closely affiliated to Paracoccus denitrificans in the lichen crust. Sequences of nosZ gene formed a distinct cluster that did not branch with known denitrifying bacteria. Our results demonstrate that nitrogen loss via denitrification is a dominant process in crusts from Oman, which leads to N 2 O gas emission and potentially reduces desert soil fertility.

Original languageEnglish
Pages (from-to)1862-1875
Number of pages14
JournalISME Journal
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Oman
Denitrification
soil crusts
soil crust
Nitrous Oxide
nitrous oxide
denitrification
Lichens
Soil
crust
lichens
denitrifying bacteria
lichen
Gases
Paracoccus denitrificans
Nitrogen
gases
Bacteria
Biological Phenomena
desert soils

Keywords

  • biological soil crust
  • denitrification
  • microsensors
  • nitrogen cycle
  • quantitative PCR
  • stable isotopes

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Microbiology

Cite this

High rates of denitrification and nitrous oxide emission in arid biological soil crusts from the Sultanate of Oman. / Abed, Raeid M M; Lam, Phyllis; De Beer, Dirk; Stief, Peter.

In: ISME Journal, Vol. 7, No. 9, 09.2013, p. 1862-1875.

Research output: Contribution to journalArticle

Abed, Raeid M M ; Lam, Phyllis ; De Beer, Dirk ; Stief, Peter. / High rates of denitrification and nitrous oxide emission in arid biological soil crusts from the Sultanate of Oman. In: ISME Journal. 2013 ; Vol. 7, No. 9. pp. 1862-1875.
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abstract = "Using a combination of process rate determination, microsensor profiling and molecular techniques, we demonstrated that denitrification, and not anaerobic ammonium oxidation (anammox), is the major nitrogen loss process in biological soil crusts from Oman. Potential denitrification rates were 584±101 and 58±20 μmol N m -2 h -1 for cyanobacterial and lichen crust, respectively. Complete denitrification to N 2 was further confirmed by an 15 NO 3 - tracer experiment with intact crust pieces that proceeded at rates of 103±19 and 27±8 μmol N m -2 h -1 for cyanobacterial and lichen crust, respectively. Strikingly, N 2 O gas was emitted at very high potential rates of 387±143 and 31±6 μmol N m -2 h -1 from the cyanobacterial and lichen crust, respectively, with N 2 O accounting for 53-66{\%} of the total emission of nitrogenous gases. Microsensor measurements revealed that N 2 O was produced in the anoxic layer and thus apparently originated from incomplete denitrification. Using quantitative PCR, denitrification genes were detected in both the crusts and were expressed either in comparable (nirS) or slightly higher (narG) numbers in the cyanobacterial crusts. Although 99{\%} of the nirS sequences in the cyanobacterial crust were affiliated to an uncultured denitrifying bacterium, 94{\%} of these sequences were most closely affiliated to Paracoccus denitrificans in the lichen crust. Sequences of nosZ gene formed a distinct cluster that did not branch with known denitrifying bacteria. Our results demonstrate that nitrogen loss via denitrification is a dominant process in crusts from Oman, which leads to N 2 O gas emission and potentially reduces desert soil fertility.",
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