High-performance liquid chromatography determination of nitrated polycyclic aromatic hydrocarbons by indirect fluorescence detection

Salma M. Al-Kindy, James N. Miller

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A high-performance liquid chromatography (HPLC) method for the analysis of nitrated polcyclic aromatic hydrocarbons (NPAHs) is reported. NPAH mixtures were pre-concentrated using solid-phase extraction and well resolved on a C18 column. They were detected using an indirect method involving the quenching of the emission from the fluorophores 5,6,7,8- tetrahydronaphthol (5,6,7,8-THN-1-OH), 7-amino-4-methyl coumarin (Coumarin 120, COU-120) and 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthylazo)2-naphthalene carboxylic acid (Calcon carboxylic acid, CCA). Linear calibration curves were obtained in the range 1.1 × 10-9 to 1.1 × 10-8 mol/L. Using COU 120 as the fluorophore, the detection limit was 2.9 × 10-10 mol/L for 1- nitronaphthalene and 2.1 × 10-11 mol/L for 2-nitrofluorene. Recoveries of NPAHs from spiked tap water samples were between 88 and 100%.

Original languageEnglish
Pages (from-to)166-169
Number of pages4
JournalBiomedical Chromatography
Volume23
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Aromatic Hydrocarbons
Polycyclic Aromatic Hydrocarbons
High performance liquid chromatography
Fluorophores
Fluorescence
High Pressure Liquid Chromatography
Carboxylic Acids
Solid Phase Extraction
Calibration
Limit of Detection
Quenching
Recovery
Water

Keywords

  • Fluorescense quenching detection
  • High-performance liquid chromatography (HPLC)
  • Nitroaromatics
  • Solid-phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology

Cite this

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abstract = "A high-performance liquid chromatography (HPLC) method for the analysis of nitrated polcyclic aromatic hydrocarbons (NPAHs) is reported. NPAH mixtures were pre-concentrated using solid-phase extraction and well resolved on a C18 column. They were detected using an indirect method involving the quenching of the emission from the fluorophores 5,6,7,8- tetrahydronaphthol (5,6,7,8-THN-1-OH), 7-amino-4-methyl coumarin (Coumarin 120, COU-120) and 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthylazo)2-naphthalene carboxylic acid (Calcon carboxylic acid, CCA). Linear calibration curves were obtained in the range 1.1 × 10-9 to 1.1 × 10-8 mol/L. Using COU 120 as the fluorophore, the detection limit was 2.9 × 10-10 mol/L for 1- nitronaphthalene and 2.1 × 10-11 mol/L for 2-nitrofluorene. Recoveries of NPAHs from spiked tap water samples were between 88 and 100{\%}.",
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AB - A high-performance liquid chromatography (HPLC) method for the analysis of nitrated polcyclic aromatic hydrocarbons (NPAHs) is reported. NPAH mixtures were pre-concentrated using solid-phase extraction and well resolved on a C18 column. They were detected using an indirect method involving the quenching of the emission from the fluorophores 5,6,7,8- tetrahydronaphthol (5,6,7,8-THN-1-OH), 7-amino-4-methyl coumarin (Coumarin 120, COU-120) and 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naphthylazo)2-naphthalene carboxylic acid (Calcon carboxylic acid, CCA). Linear calibration curves were obtained in the range 1.1 × 10-9 to 1.1 × 10-8 mol/L. Using COU 120 as the fluorophore, the detection limit was 2.9 × 10-10 mol/L for 1- nitronaphthalene and 2.1 × 10-11 mol/L for 2-nitrofluorene. Recoveries of NPAHs from spiked tap water samples were between 88 and 100%.

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