High-performance liquid chromatographic method for quantification of busulfan in plasma after derivatization by tetrafluorothiophenol

Marie Hélène Quernin, Balasubramanian Poonkuzhali, Yves Médard, David Dennison, Alok Srivastava, Rajagopal Krishnamoorthy, Mammen Chandy, Evelyne Jacqz-Aigrain

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A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of busulfan in plasma. Busulfan was extracted in toluene, derivatized by 2,3,5,6-tetrafluorothiophenol to obtain di-TFTP-butane, the derivatization product was then re-extracted in toluene and injected into the HPLC system with ultraviolet detection (wavelength: 275 nm). Recovery from extraction was 80%, the limit of quantification was 50 ng/ml and linearity ranged from 50 to 2000 ng/ml. In addition, forty-two samples obtained from pediatric patients treated with busulfan were analyzed by the HPLC and GC-MS assays based on the same derivatization procedure. The correlation between the di-TFTP-butane concentrations was highly significant (p<0.0001), demonstrating that the two methods were in good agreement. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Issue number1
Publication statusPublished - Jan 8 1999



  • Busulfan
  • Derivatization, LC

ASJC Scopus subject areas

  • Chemistry(all)

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