Abstract
A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of busulfan in plasma. Busulfan was extracted in toluene, derivatized by 2,3,5,6-tetrafluorothiophenol to obtain di-TFTP-butane, the derivatization product was then re-extracted in toluene and injected into the HPLC system with ultraviolet detection (wavelength: 275 nm). Recovery from extraction was 80%, the limit of quantification was 50 ng/ml and linearity ranged from 50 to 2000 ng/ml. In addition, forty-two samples obtained from pediatric patients treated with busulfan were analyzed by the HPLC and GC-MS assays based on the same derivatization procedure. The correlation between the di-TFTP-butane concentrations was highly significant (p<0.0001), demonstrating that the two methods were in good agreement. Copyright (C) 1999 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 147-152 |
| Number of pages | 6 |
| Journal | Journal of Chromatography B: Biomedical Applications |
| Volume | 721 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 8 1999 |
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Keywords
- Busulfan
- Derivatization, LC
ASJC Scopus subject areas
- Chemistry(all)
Cite this
High-performance liquid chromatographic method for quantification of busulfan in plasma after derivatization by tetrafluorothiophenol. / Quernin, Marie Hélène; Poonkuzhali, Balasubramanian; Médard, Yves; Dennison, David; Srivastava, Alok; Krishnamoorthy, Rajagopal; Chandy, Mammen; Jacqz-Aigrain, Evelyne.
In: Journal of Chromatography B: Biomedical Applications, Vol. 721, No. 1, 08.01.1999, p. 147-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-performance liquid chromatographic method for quantification of busulfan in plasma after derivatization by tetrafluorothiophenol
AU - Quernin, Marie Hélène
AU - Poonkuzhali, Balasubramanian
AU - Médard, Yves
AU - Dennison, David
AU - Srivastava, Alok
AU - Krishnamoorthy, Rajagopal
AU - Chandy, Mammen
AU - Jacqz-Aigrain, Evelyne
PY - 1999/1/8
Y1 - 1999/1/8
N2 - A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of busulfan in plasma. Busulfan was extracted in toluene, derivatized by 2,3,5,6-tetrafluorothiophenol to obtain di-TFTP-butane, the derivatization product was then re-extracted in toluene and injected into the HPLC system with ultraviolet detection (wavelength: 275 nm). Recovery from extraction was 80%, the limit of quantification was 50 ng/ml and linearity ranged from 50 to 2000 ng/ml. In addition, forty-two samples obtained from pediatric patients treated with busulfan were analyzed by the HPLC and GC-MS assays based on the same derivatization procedure. The correlation between the di-TFTP-butane concentrations was highly significant (p<0.0001), demonstrating that the two methods were in good agreement. Copyright (C) 1999 Elsevier Science B.V.
AB - A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of busulfan in plasma. Busulfan was extracted in toluene, derivatized by 2,3,5,6-tetrafluorothiophenol to obtain di-TFTP-butane, the derivatization product was then re-extracted in toluene and injected into the HPLC system with ultraviolet detection (wavelength: 275 nm). Recovery from extraction was 80%, the limit of quantification was 50 ng/ml and linearity ranged from 50 to 2000 ng/ml. In addition, forty-two samples obtained from pediatric patients treated with busulfan were analyzed by the HPLC and GC-MS assays based on the same derivatization procedure. The correlation between the di-TFTP-butane concentrations was highly significant (p<0.0001), demonstrating that the two methods were in good agreement. Copyright (C) 1999 Elsevier Science B.V.
KW - Busulfan
KW - Derivatization, LC
UR - http://www.scopus.com/inward/record.url?scp=0032958943&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032958943&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(98)00473-3
DO - 10.1016/S0378-4347(98)00473-3
M3 - Article
VL - 721
SP - 147
EP - 152
JO - Journal of Chromatography - Biomedical Applications
JF - Journal of Chromatography - Biomedical Applications
SN - 0378-4347
IS - 1
ER -