TY - JOUR
T1 - High-performance liquid chromatographic method for quantification of busulfan in plasma after derivatization by tetrafluorothiophenol
AU - Quernin, Marie Hélène
AU - Poonkuzhali, Balasubramanian
AU - Médard, Yves
AU - Dennison, David
AU - Srivastava, Alok
AU - Krishnamoorthy, Rajagopal
AU - Chandy, Mammen
AU - Jacqz-Aigrain, Evelyne
N1 - Funding Information:
This Indo–French network was made possible by the financial assistance from Inserm, France, under the grant no. 94NS4 attributed to Dr. R. Krishnamoorthy and the Indian Council of Medical Research for the ‘‘Advanced Center for Bone Marrow Transplantation for Thalassaemia in India’’ at Christian Medical College and Hospital, Vellore, India.
PY - 1999/1/8
Y1 - 1999/1/8
N2 - A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of busulfan in plasma. Busulfan was extracted in toluene, derivatized by 2,3,5,6-tetrafluorothiophenol to obtain di-TFTP-butane, the derivatization product was then re-extracted in toluene and injected into the HPLC system with ultraviolet detection (wavelength: 275 nm). Recovery from extraction was 80%, the limit of quantification was 50 ng/ml and linearity ranged from 50 to 2000 ng/ml. In addition, forty-two samples obtained from pediatric patients treated with busulfan were analyzed by the HPLC and GC-MS assays based on the same derivatization procedure. The correlation between the di-TFTP-butane concentrations was highly significant (p<0.0001), demonstrating that the two methods were in good agreement. Copyright (C) 1999 Elsevier Science B.V.
AB - A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of busulfan in plasma. Busulfan was extracted in toluene, derivatized by 2,3,5,6-tetrafluorothiophenol to obtain di-TFTP-butane, the derivatization product was then re-extracted in toluene and injected into the HPLC system with ultraviolet detection (wavelength: 275 nm). Recovery from extraction was 80%, the limit of quantification was 50 ng/ml and linearity ranged from 50 to 2000 ng/ml. In addition, forty-two samples obtained from pediatric patients treated with busulfan were analyzed by the HPLC and GC-MS assays based on the same derivatization procedure. The correlation between the di-TFTP-butane concentrations was highly significant (p<0.0001), demonstrating that the two methods were in good agreement. Copyright (C) 1999 Elsevier Science B.V.
KW - Busulfan
KW - Derivatization, LC
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U2 - 10.1016/S0378-4347(98)00473-3
DO - 10.1016/S0378-4347(98)00473-3
M3 - Article
C2 - 10027646
AN - SCOPUS:0032958943
VL - 721
SP - 147
EP - 152
JO - Journal of Chromatography B: Biomedical Applications
JF - Journal of Chromatography B: Biomedical Applications
SN - 0378-4347
IS - 1
ER -