Hiding in Fresh Fruits and Vegetables

Opportunistic Pathogens May Cross Geographical Barriers

Zahra S. Al-Kharousi, Nejib Guizani, Abdullah M. Al-Sadi, Ismail M. Al-Bulushi, Baby Shaharoona

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Different microbial groups of the microbiome of fresh produce can have diverse effects on human health. This study was aimed at identifying some microbial communities of fresh produce by analyzing 105 samples of imported fresh fruits and vegetables originated from different countries in the world including local samples (Oman) for aerobic plate count and the counts of Enterobacteriaceae, Enterococcus, and Staphylococcus aureus. The isolated bacteria were identified by molecular (PCR) and biochemical methods (VITEK 2). Enterobacteriaceae occurred in 60% of fruits and 91% of vegetables. Enterococcus was isolated from 20% of fruits and 42% of vegetables. E. coli and S. aureus were isolated from 22% and 7% of vegetables, respectively. Ninety-seven bacteria comprising 21 species were similarly identified by VITEK 2 and PCR to species level. E. coli, Klebsiella pneumoniae, Enterococcus casseliflavus, and Enterobacter cloacae were the most abundant species; many are known as opportunistic pathogens which may raise concern to improve the microbial quality of fresh produce. Phylogenetic trees showed no relationship between clustering of the isolates based on the 16S rRNA gene and the original countries of fresh produce. Intercountry passage of opportunistic pathogens in fresh produce cannot be ruled out, which requires better management.

Original languageEnglish
Article number4292417
JournalInternational Journal of Microbiology
Volume2016
DOIs
Publication statusPublished - 2016

Fingerprint

Vegetables
Fruit
Enterococcus
Enterobacteriaceae
Staphylococcus aureus
Oman
Escherichia coli
Bacteria
Enterobacter cloacae
Polymerase Chain Reaction
Microbiota
Klebsiella pneumoniae
rRNA Genes
Cluster Analysis
Health

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

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title = "Hiding in Fresh Fruits and Vegetables: Opportunistic Pathogens May Cross Geographical Barriers",
abstract = "Different microbial groups of the microbiome of fresh produce can have diverse effects on human health. This study was aimed at identifying some microbial communities of fresh produce by analyzing 105 samples of imported fresh fruits and vegetables originated from different countries in the world including local samples (Oman) for aerobic plate count and the counts of Enterobacteriaceae, Enterococcus, and Staphylococcus aureus. The isolated bacteria were identified by molecular (PCR) and biochemical methods (VITEK 2). Enterobacteriaceae occurred in 60{\%} of fruits and 91{\%} of vegetables. Enterococcus was isolated from 20{\%} of fruits and 42{\%} of vegetables. E. coli and S. aureus were isolated from 22{\%} and 7{\%} of vegetables, respectively. Ninety-seven bacteria comprising 21 species were similarly identified by VITEK 2 and PCR to species level. E. coli, Klebsiella pneumoniae, Enterococcus casseliflavus, and Enterobacter cloacae were the most abundant species; many are known as opportunistic pathogens which may raise concern to improve the microbial quality of fresh produce. Phylogenetic trees showed no relationship between clustering of the isolates based on the 16S rRNA gene and the original countries of fresh produce. Intercountry passage of opportunistic pathogens in fresh produce cannot be ruled out, which requires better management.",
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