Gum acacia mitigates genetic damage in adenine-induced chronic renal failure in rats

B. H. Ali, K. Al Balushi, I. Al-Husseini, P. Mandel, A. Nemmar, N. Schupp, D. A. Ribeiro

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Subjects with chronic renal failure (CRF) exhibit oxidative genome damage, which may predispose to carcinogenesis, and Gum acacia (GumA) ameliorates this condition in humans and animals. We evaluated here renal DNA damage and urinary excretion of four nucleic acid oxidation adducts namely 8-oxoguanine (8-oxoGua), 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxoguanosine (8-oxoGuo) and 8-hydroxy-2-deoxyguanisone (8-OHdg) in rats with adenine (ADE)-induced CRF with and without GumA treatment. Materials and Methods: Twenty-four rats were divided into four equal groups and treated for 4 weeks. The first group was given normal food and water (control). The second group was given normal food and GumA (15% w/v) in drinking water. The third group was fed powder diet containing adenine (ADE) (0·75% w/w in feed). The fourth group was fed like in the third group, plus GumA in drinking water (15%, w/v). Results: ADE feeding induced CRF (as measured by several physiological, biochemical and histological indices) and also caused a significant genetic damage and significant decreases in urinary 8-oxo Gua and 8-oxoGuo, but not in the other nucleic acids. However, concomitant GumA treatment reduced the level of genetic damage in kidney cells as detected by Comet assay and significantly reversed the effect of adenine on urinary 8-oxoGuo. Conclusions: Treatment with GumA is able to mitigate genetic damage in renal tissues of rats with ADE-induced CRF.

Original languageEnglish
Pages (from-to)1221-1227
Number of pages7
JournalEuropean Journal of Clinical Investigation
Volume45
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Gum Arabic
Adenine
Chronic Kidney Failure
Rats
Kidney
Drinking Water
Nucleic Acids
Food
Comet Assay
Nutrition
Powders
DNA Damage
Assays
Carcinogenesis
Animals
Genes
Genome
Tissue
Diet
Oxidation

Keywords

  • Adenine
  • Chronic renal failure
  • Comet test
  • DNA damage
  • Gum acacia
  • Rats

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Biochemistry
  • Biochemistry

Cite this

Gum acacia mitigates genetic damage in adenine-induced chronic renal failure in rats. / Ali, B. H.; Al Balushi, K.; Al-Husseini, I.; Mandel, P.; Nemmar, A.; Schupp, N.; Ribeiro, D. A.

In: European Journal of Clinical Investigation, Vol. 45, No. 12, 01.12.2015, p. 1221-1227.

Research output: Contribution to journalArticle

Ali, B. H. ; Al Balushi, K. ; Al-Husseini, I. ; Mandel, P. ; Nemmar, A. ; Schupp, N. ; Ribeiro, D. A. / Gum acacia mitigates genetic damage in adenine-induced chronic renal failure in rats. In: European Journal of Clinical Investigation. 2015 ; Vol. 45, No. 12. pp. 1221-1227.
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abstract = "Background: Subjects with chronic renal failure (CRF) exhibit oxidative genome damage, which may predispose to carcinogenesis, and Gum acacia (GumA) ameliorates this condition in humans and animals. We evaluated here renal DNA damage and urinary excretion of four nucleic acid oxidation adducts namely 8-oxoguanine (8-oxoGua), 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxoguanosine (8-oxoGuo) and 8-hydroxy-2-deoxyguanisone (8-OHdg) in rats with adenine (ADE)-induced CRF with and without GumA treatment. Materials and Methods: Twenty-four rats were divided into four equal groups and treated for 4 weeks. The first group was given normal food and water (control). The second group was given normal food and GumA (15{\%} w/v) in drinking water. The third group was fed powder diet containing adenine (ADE) (0·75{\%} w/w in feed). The fourth group was fed like in the third group, plus GumA in drinking water (15{\%}, w/v). Results: ADE feeding induced CRF (as measured by several physiological, biochemical and histological indices) and also caused a significant genetic damage and significant decreases in urinary 8-oxo Gua and 8-oxoGuo, but not in the other nucleic acids. However, concomitant GumA treatment reduced the level of genetic damage in kidney cells as detected by Comet assay and significantly reversed the effect of adenine on urinary 8-oxoGuo. Conclusions: Treatment with GumA is able to mitigate genetic damage in renal tissues of rats with ADE-induced CRF.",
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AU - Ali, B. H.

AU - Al Balushi, K.

AU - Al-Husseini, I.

AU - Mandel, P.

AU - Nemmar, A.

AU - Schupp, N.

AU - Ribeiro, D. A.

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N2 - Background: Subjects with chronic renal failure (CRF) exhibit oxidative genome damage, which may predispose to carcinogenesis, and Gum acacia (GumA) ameliorates this condition in humans and animals. We evaluated here renal DNA damage and urinary excretion of four nucleic acid oxidation adducts namely 8-oxoguanine (8-oxoGua), 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxoguanosine (8-oxoGuo) and 8-hydroxy-2-deoxyguanisone (8-OHdg) in rats with adenine (ADE)-induced CRF with and without GumA treatment. Materials and Methods: Twenty-four rats were divided into four equal groups and treated for 4 weeks. The first group was given normal food and water (control). The second group was given normal food and GumA (15% w/v) in drinking water. The third group was fed powder diet containing adenine (ADE) (0·75% w/w in feed). The fourth group was fed like in the third group, plus GumA in drinking water (15%, w/v). Results: ADE feeding induced CRF (as measured by several physiological, biochemical and histological indices) and also caused a significant genetic damage and significant decreases in urinary 8-oxo Gua and 8-oxoGuo, but not in the other nucleic acids. However, concomitant GumA treatment reduced the level of genetic damage in kidney cells as detected by Comet assay and significantly reversed the effect of adenine on urinary 8-oxoGuo. Conclusions: Treatment with GumA is able to mitigate genetic damage in renal tissues of rats with ADE-induced CRF.

AB - Background: Subjects with chronic renal failure (CRF) exhibit oxidative genome damage, which may predispose to carcinogenesis, and Gum acacia (GumA) ameliorates this condition in humans and animals. We evaluated here renal DNA damage and urinary excretion of four nucleic acid oxidation adducts namely 8-oxoguanine (8-oxoGua), 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxoguanosine (8-oxoGuo) and 8-hydroxy-2-deoxyguanisone (8-OHdg) in rats with adenine (ADE)-induced CRF with and without GumA treatment. Materials and Methods: Twenty-four rats were divided into four equal groups and treated for 4 weeks. The first group was given normal food and water (control). The second group was given normal food and GumA (15% w/v) in drinking water. The third group was fed powder diet containing adenine (ADE) (0·75% w/w in feed). The fourth group was fed like in the third group, plus GumA in drinking water (15%, w/v). Results: ADE feeding induced CRF (as measured by several physiological, biochemical and histological indices) and also caused a significant genetic damage and significant decreases in urinary 8-oxo Gua and 8-oxoGuo, but not in the other nucleic acids. However, concomitant GumA treatment reduced the level of genetic damage in kidney cells as detected by Comet assay and significantly reversed the effect of adenine on urinary 8-oxoGuo. Conclusions: Treatment with GumA is able to mitigate genetic damage in renal tissues of rats with ADE-induced CRF.

KW - Adenine

KW - Chronic renal failure

KW - Comet test

KW - DNA damage

KW - Gum acacia

KW - Rats

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