Gonadotropin-releasing hormone receptor gene expression in rat anterior pituitary

Hichem C. Mertani, Catherine Testart, Allal Ouhtit, Christine Brisson, Gérard Morel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Gonadotropin-releasing hormone (GnRH) effects on the lactotroph function have been widely studied, but they probably result from paracrine interactions. No visual data about GnRH receptor in the pituitary are available. In order to identify the GnRH target cells in the pituitary of adult rats, the cellular distribution of rat GnRH receptor mRNA was investigated by electron microscopy, using in situ hybridization on ultrathin pituitary frozen sections. In situ hybridization was performed using a digoxigenin-labeled oligonucleotide probe revealed by an indirect immunogold reaction. Gonadotropin-releasing hormone receptor mRNA was found in the cytoplasmic matrix, apposed to the endoplasmic reticulum and the nucleus of the gonadotrophs, which were identified by their ultrastructural characteristics, and by the presence of luteinizing hormone (LH) immunoreactivity. It was also found in the lactotrophs, which were revealed by the immunocytological detection of prolactin. No GnRH receptor mRNA was detected in corticotrophs, somatotrophs, thyrotrophs or hepatocytes. This result, without excluding paracrine effects, clearly showed that in addition to the gonadotrophs, the lactototrophs are likely to be direct target cells for the hypothalamic GnRH.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalEndocrine
Volume4
Issue number2
DOIs
Publication statusPublished - Apr 1996

Fingerprint

LHRH Receptors
Gonadotropin-Releasing Hormone
Lactotrophs
Gonadotrophs
Gene Expression
Messenger RNA
In Situ Hybridization
Thyrotrophs
Corticotrophs
Somatotrophs
Pituitary Hormone-Releasing Hormones
Digoxigenin
Oligonucleotide Probes
Frozen Sections
Luteinizing Hormone
Endoplasmic Reticulum
Prolactin
Hepatocytes
Electron Microscopy

Keywords

  • electron microscopy
  • Gonadotropin-releasing hormone receptor
  • immunocytology
  • in situ hybridization
  • mRNA
  • paracrine
  • rat anterior pituitary

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Mertani, H. C., Testart, C., Ouhtit, A., Brisson, C., & Morel, G. (1996). Gonadotropin-releasing hormone receptor gene expression in rat anterior pituitary. Endocrine, 4(2), 159-163. https://doi.org/10.1007/BF02782760

Gonadotropin-releasing hormone receptor gene expression in rat anterior pituitary. / Mertani, Hichem C.; Testart, Catherine; Ouhtit, Allal; Brisson, Christine; Morel, Gérard.

In: Endocrine, Vol. 4, No. 2, 04.1996, p. 159-163.

Research output: Contribution to journalArticle

Mertani, HC, Testart, C, Ouhtit, A, Brisson, C & Morel, G 1996, 'Gonadotropin-releasing hormone receptor gene expression in rat anterior pituitary', Endocrine, vol. 4, no. 2, pp. 159-163. https://doi.org/10.1007/BF02782760
Mertani, Hichem C. ; Testart, Catherine ; Ouhtit, Allal ; Brisson, Christine ; Morel, Gérard. / Gonadotropin-releasing hormone receptor gene expression in rat anterior pituitary. In: Endocrine. 1996 ; Vol. 4, No. 2. pp. 159-163.
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