Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction

Michela Menegon, Carlo Severini, Anna Sannella, Maria Grazia Paglia, Djibril Sangaré, Amel Abdel-Wahab, Abdel Muhsin A Abdel-Muhsin, Hamza Babiker, David Walliker, Pietro Alano

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40 Citations (Scopus)

Abstract

A molecular assay has been developed for the specific detection and genetic characterisation of Plasmodium falciparum gametocytes in the blood of malaria infected individuals. The assay is based on the reverse transcription and polymerase chain reaction (RT-PCR) amplification of the messenger RNA of gene pfg377, a sexual-stage specific transcript abundantly produced in maturing gametocytes. The gene contains four regions of repetitive sequences, of which region 3 was shown to be the most polymorphic in laboratory clones and field isolates of the parasite. Analysis of samples of malaria infected blood by RT-PCR specific for region 3 has enabled identification of multiple gametocyte-producing clones within single infections. The assay is able to detect gametocytes below the threshold of microscopic detection, and is highly specific for its gametocyte targets also in the presence of a vast excess of asexual forms. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume111
Issue number1
DOIs
Publication statusPublished - 2000

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Keywords

  • Gametocyte carrier
  • Genetic diversity
  • pfg377 mRNA
  • Plasmodium falciparum
  • RT-PCR assay
  • Transmission stages

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Menegon, M., Severini, C., Sannella, A., Paglia, M. G., Sangaré, D., Abdel-Wahab, A., ... Alano, P. (2000). Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction. Molecular and Biochemical Parasitology, 111(1), 153-161. https://doi.org/10.1016/S0166-6851(00)00314-5