Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

Jørgen Stenvik, Mette Serine Wesmajervi, Børge Damsgård, Madjid Delghandi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The two main allelic variants of the Atlantic cod (Gadus morhua L.) pantophysin I (Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele-specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping.

Original languageEnglish
Pages (from-to)272-275
Number of pages4
JournalMolecular Ecology Notes
Volume6
Issue number1
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Gadus morhua
Polymerase chain reaction
Polymorphism
polymerase chain reaction
genotyping
Gadiformes
allele
Alleles
Throughput
cod (fish)
alleles
Polymerase Chain Reaction
DNA
Costs
duplex
polymorphism
methodology
genetic polymorphism
Costs and Cost Analysis
loci

Keywords

  • Allele-specific PCR
  • Atlantic cod
  • Genotyping
  • Pantophysin I

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Ecology

Cite this

Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR. / Stenvik, Jørgen; Wesmajervi, Mette Serine; Damsgård, Børge; Delghandi, Madjid.

In: Molecular Ecology Notes, Vol. 6, No. 1, 03.2006, p. 272-275.

Research output: Contribution to journalArticle

Stenvik, Jørgen ; Wesmajervi, Mette Serine ; Damsgård, Børge ; Delghandi, Madjid. / Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR. In: Molecular Ecology Notes. 2006 ; Vol. 6, No. 1. pp. 272-275.
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