Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

Jørgen Stenvik, Mette Serine Wesmajervi, Børge Damsgård, Madjid Delghandi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The two main allelic variants of the Atlantic cod (Gadus morhua L.) pantophysin I (Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele-specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping.

Original languageEnglish
Pages (from-to)272-275
Number of pages4
JournalMolecular Ecology Notes
Volume6
Issue number1
DOIs
Publication statusPublished - Mar 2006

Keywords

  • Allele-specific PCR
  • Atlantic cod
  • Genotyping
  • Pantophysin I

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Ecology

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