Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

Jørgen Stenvik; Mette Serine Wesmajervi; Børge Damsgård; Madjid Delghandi

doi:10.1111/j.1471-8286.2005.01178.x

Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

Jørgen Stenvik, Mette Serine Wesmajervi, Børge Damsgård, Madjid Delghandi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The two main allelic variants of the Atlantic cod (Gadus morhua L.) pantophysin I (Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele-specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping.

Original language	English
Pages (from-to)	272-275
Number of pages	4
Journal	Molecular Ecology Notes
Volume	6
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-8286.2005.01178.x
Publication status	Published - Mar 2006
Externally published	Yes

Keywords

Allele-specific PCR
Atlantic cod
Genotyping
Pantophysin I

ASJC Scopus subject areas

Biochemistry
Ecology
Biochemistry, Genetics and Molecular Biology(all)

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10.1111/j.1471-8286.2005.01178.x

Cite this

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abstract = "The two main allelic variants of the Atlantic cod (Gadus morhua L.) pantophysin I (Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele-specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping.",

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year = "2006",

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T1 - Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

AU - Stenvik, Jørgen

AU - Wesmajervi, Mette Serine

AU - Damsgård, Børge

AU - Delghandi, Madjid

PY - 2006/3

Y1 - 2006/3

N2 - The two main allelic variants of the Atlantic cod (Gadus morhua L.) pantophysin I (Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele-specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping.

AB - The two main allelic variants of the Atlantic cod (Gadus morhua L.) pantophysin I (Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele-specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost-effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping.

KW - Allele-specific PCR

KW - Atlantic cod

KW - Genotyping

KW - Pantophysin I

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Genotyping of pantophysin I (Pan I) of Atlantic cod (Gadus morhua L.) by allele-specific PCR

Abstract

Keywords

ASJC Scopus subject areas

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