TY - JOUR
T1 - Genome-wide identification and functional characterization of glutathione peroxidase genes in date palm (Phoenix dactylifera L.) under stress conditions
AU - Jana, Gerry Aplang
AU - Yaish, Mahmoud W.
N1 - Funding Information:
Authors would like to acknowledge the College of Science, Sultan Qaboos University, Oman, for the generous fund number IG/SCI/BIOL/18/01 to MWY. Authors would also like to thank Dr. Sirin A. Adham and the Central Analytical and Applied Research Unit (CAARU), SQU, for the help in flow cytometer analysis.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/9
Y1 - 2020/9
N2 - Glutathione peroxidase (GPX) (EC 1.11.1.9) is a group of antioxidant enzymes that catalyze the reduction of hydrogen peroxide (H2O2) and lipid peroxidase to water and lipid alcohols, respectively. GPX enzymes are indispensable in protecting the cell from reactive oxygen species (ROS) damage, stress signaling and for maintaining cellular redox balance under stress conditions. Date palm (Phoenix dactylifera L.) is an important fruit-producing tree in the Arabian Peninsula, which can tolerate extreme environmental conditions, including salinity and drought conditions. This tolerance trait may be attributed to the various antioxidant enzyme system of the plant. Therefore, in this study, we aim to understand the contribution of the GPX gene family to the antioxidant system present in date palms. Five putative PdGPX genes (PdGPX1–5) were mined from the date palm genome, computationally analyzed, and their expression profiles under salinity, oxidative and methylglyoxal stresses, were determined using qPCR. The identified PdGPX genes were then functionally characterized at the molecular level in Escherichia coli (BL21) and the yeast (Saccharomyces cerevisiae) knockout cell line (GPX3Δ). The results showed that the PdGPX gene family is conserved in the date palm genome and their expression was altered based on the stress used in the experiment and plant tissue. Furthermore, heterologous expression of the PdGPX genes enhanced the survival of the E. coli (BL21) under different stresses, including salinity (250 mM NaCl), toxicity (0.5 mM methylglyoxal (MG) and oxidative stress (5 mM H2O2). The putative PdGPX genes were also able to complement the loss in function of the yeast knockout lines (GPX3Δ) by enhancing their survival under H2O2 and MG stress. These findings indicated that the PdGPX family plays a vital role in the antioxidant activity, which may enhance the survival of the date palm under stress conditions.
AB - Glutathione peroxidase (GPX) (EC 1.11.1.9) is a group of antioxidant enzymes that catalyze the reduction of hydrogen peroxide (H2O2) and lipid peroxidase to water and lipid alcohols, respectively. GPX enzymes are indispensable in protecting the cell from reactive oxygen species (ROS) damage, stress signaling and for maintaining cellular redox balance under stress conditions. Date palm (Phoenix dactylifera L.) is an important fruit-producing tree in the Arabian Peninsula, which can tolerate extreme environmental conditions, including salinity and drought conditions. This tolerance trait may be attributed to the various antioxidant enzyme system of the plant. Therefore, in this study, we aim to understand the contribution of the GPX gene family to the antioxidant system present in date palms. Five putative PdGPX genes (PdGPX1–5) were mined from the date palm genome, computationally analyzed, and their expression profiles under salinity, oxidative and methylglyoxal stresses, were determined using qPCR. The identified PdGPX genes were then functionally characterized at the molecular level in Escherichia coli (BL21) and the yeast (Saccharomyces cerevisiae) knockout cell line (GPX3Δ). The results showed that the PdGPX gene family is conserved in the date palm genome and their expression was altered based on the stress used in the experiment and plant tissue. Furthermore, heterologous expression of the PdGPX genes enhanced the survival of the E. coli (BL21) under different stresses, including salinity (250 mM NaCl), toxicity (0.5 mM methylglyoxal (MG) and oxidative stress (5 mM H2O2). The putative PdGPX genes were also able to complement the loss in function of the yeast knockout lines (GPX3Δ) by enhancing their survival under H2O2 and MG stress. These findings indicated that the PdGPX family plays a vital role in the antioxidant activity, which may enhance the survival of the date palm under stress conditions.
KW - Abiotic stress
KW - Date palm, salinity
KW - Glutathione peroxidase
KW - Oxidative stress
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U2 - 10.1016/j.plgene.2020.100237
DO - 10.1016/j.plgene.2020.100237
M3 - Article
AN - SCOPUS:85086432696
SN - 2352-4073
VL - 23
JO - Plant Gene
JF - Plant Gene
M1 - 100237
ER -