Genome-wide expression profiling in leaves and roots of date palm (Phoenix dactylifera L.) exposed to salinity

Mahmoud W. Yaish, Himanshu V. Patankar, Dekoum V M Assaha, Yun Zheng, Rashid Al-Yahyai, Ramanjulu Sunkar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Date palm, as one of the most important fruit crops in North African and West Asian countries including Oman, is facing serious growth problems due to salinity, arising from persistent use of saline water for irrigation. Although date palm is a relatively salt-tolerant plant species, its adaptive mechanisms to salt stress are largely unknown. Results: In order to get an insight into molecular mechanisms of salt tolerance, RNA was profiled in leaves and roots of date palm seedlings subjected to NaCl for 10 days. Under salt stress, photosynthetic parameters were differentially affected; all gas exchange parameters were decreased but the quantum yield of PSII was unaffected while non-photochemical quenching was increased. Analyses of gene expression profiles revealed 2630 and 4687 genes were differentially expressed in leaves and roots, respectively, under salt stress. Of these, 194 genes were identified as commonly responding in both the tissue sources. Gene ontology (GO) analysis in leaves revealed enrichment of transcripts involved in metabolic pathways including photosynthesis, sucrose and starch metabolism, and oxidative phosphorylation, while in roots genes involved in membrane transport, phenylpropanoid biosynthesis, purine, thiamine, and tryptophan metabolism, and casparian strip development were enriched. Differentially expressed genes (DEGs) common to both tissues included the auxin responsive gene, GH3, a putative potassium transporter 8 and vacuolar membrane proton pump. Conclusions: Leaf and root tissues respond differentially to salinity stress and this study has revealed genes and pathways that are associated with responses to elevated NaCl levels and thus may play important roles in salt tolerance providing a foundation for functional characterization of salt stress-responsive genes in the date palm.

Original languageEnglish
Article number246
JournalBMC Genomics
Volume18
Issue number1
DOIs
Publication statusPublished - Mar 22 2017

Fingerprint

Salinity
Genome
Genes
Salts
Salt-Tolerance
Salt-Tolerant Plants
Oman
Proton Pumps
Gene Ontology
Indoleacetic Acids
Membranes
Thiamine
Oxidative Phosphorylation
Photosynthesis
Metabolic Networks and Pathways
Phoeniceae
Seedlings
Transcriptome
Tryptophan
Starch

Keywords

  • Date palm
  • DEGs
  • Differentially expressed genes
  • Leaves
  • RNA-seq
  • Roots
  • Salinity

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Genome-wide expression profiling in leaves and roots of date palm (Phoenix dactylifera L.) exposed to salinity. / Yaish, Mahmoud W.; Patankar, Himanshu V.; Assaha, Dekoum V M; Zheng, Yun; Al-Yahyai, Rashid; Sunkar, Ramanjulu.

In: BMC Genomics, Vol. 18, No. 1, 246, 22.03.2017.

Research output: Contribution to journalArticle

Yaish, Mahmoud W. ; Patankar, Himanshu V. ; Assaha, Dekoum V M ; Zheng, Yun ; Al-Yahyai, Rashid ; Sunkar, Ramanjulu. / Genome-wide expression profiling in leaves and roots of date palm (Phoenix dactylifera L.) exposed to salinity. In: BMC Genomics. 2017 ; Vol. 18, No. 1.
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