Abstract
Ninety three recombinant inbreds of Sorghum bicolor (L. Moench) were derived from a cross between two sorghum lines GBIK and Redlan. This population was used to identify quantitative trait loci (QTLs) for resistance and tolerance to greenbug (Schizaphids graminum Rondani) Biotypes I and K. One hundred and thirteen loci (38 SSRs and 75 RAPDs) were mapped in 12 linkage groups covering 1,530 cM. In general, nine QTLs were detected affecting both resistance and tolerance to greenbug (GB) Biotypes I and K. The phenotypic variance explained by each QTL ranged from 5.6% to 38.4%. Four SSRs and one RAPD marker were associated with the expression of all resistance and tolerance traits. These markers appear to be linked to biotype nonspecific resistance and tolerance genes. Four additional markers were associated with biotype-specific resistance or tolerance traits. The detection of more than one locus for each biotype supports the hypothesis that several regions, which represent different genes, control the expression of resistance and tolerance to greenbug in sorghum. The results can be used for marker-assisted selection and the breeding of greenbug-tolerant sorghum cultivars.
| Original language | English |
|---|---|
| Pages (from-to) | 1373-1378 |
| Number of pages | 6 |
| Journal | Theoretical And Applied Genetics |
| Volume | 104 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- (SSRs)
- RAPDs
- Schizaphids graminum
- Simple sequence repeats
- Sorghum bicolor
- SPAD
ASJC Scopus subject areas
- Biotechnology
- Agronomy and Crop Science
- Genetics
Cite this
Genetic mapping of QTLs associated with greenbug resistance and tolerance in Sorghum bicolor. / Agrama, H. A.; Widle, G. E.; Reese, J. C.; Campbell, L. R.; Tuinstra, M. R.
In: Theoretical And Applied Genetics, Vol. 104, No. 8, 2002, p. 1373-1378.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic mapping of QTLs associated with greenbug resistance and tolerance in Sorghum bicolor
AU - Agrama, H. A.
AU - Widle, G. E.
AU - Reese, J. C.
AU - Campbell, L. R.
AU - Tuinstra, M. R.
PY - 2002
Y1 - 2002
N2 - Ninety three recombinant inbreds of Sorghum bicolor (L. Moench) were derived from a cross between two sorghum lines GBIK and Redlan. This population was used to identify quantitative trait loci (QTLs) for resistance and tolerance to greenbug (Schizaphids graminum Rondani) Biotypes I and K. One hundred and thirteen loci (38 SSRs and 75 RAPDs) were mapped in 12 linkage groups covering 1,530 cM. In general, nine QTLs were detected affecting both resistance and tolerance to greenbug (GB) Biotypes I and K. The phenotypic variance explained by each QTL ranged from 5.6% to 38.4%. Four SSRs and one RAPD marker were associated with the expression of all resistance and tolerance traits. These markers appear to be linked to biotype nonspecific resistance and tolerance genes. Four additional markers were associated with biotype-specific resistance or tolerance traits. The detection of more than one locus for each biotype supports the hypothesis that several regions, which represent different genes, control the expression of resistance and tolerance to greenbug in sorghum. The results can be used for marker-assisted selection and the breeding of greenbug-tolerant sorghum cultivars.
AB - Ninety three recombinant inbreds of Sorghum bicolor (L. Moench) were derived from a cross between two sorghum lines GBIK and Redlan. This population was used to identify quantitative trait loci (QTLs) for resistance and tolerance to greenbug (Schizaphids graminum Rondani) Biotypes I and K. One hundred and thirteen loci (38 SSRs and 75 RAPDs) were mapped in 12 linkage groups covering 1,530 cM. In general, nine QTLs were detected affecting both resistance and tolerance to greenbug (GB) Biotypes I and K. The phenotypic variance explained by each QTL ranged from 5.6% to 38.4%. Four SSRs and one RAPD marker were associated with the expression of all resistance and tolerance traits. These markers appear to be linked to biotype nonspecific resistance and tolerance genes. Four additional markers were associated with biotype-specific resistance or tolerance traits. The detection of more than one locus for each biotype supports the hypothesis that several regions, which represent different genes, control the expression of resistance and tolerance to greenbug in sorghum. The results can be used for marker-assisted selection and the breeding of greenbug-tolerant sorghum cultivars.
KW - (SSRs)
KW - RAPDs
KW - Schizaphids graminum
KW - Simple sequence repeats
KW - Sorghum bicolor
KW - SPAD
UR - http://www.scopus.com/inward/record.url?scp=0347297323&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347297323&partnerID=8YFLogxK
U2 - 10.1007/s00122-002-0923-3
DO - 10.1007/s00122-002-0923-3
M3 - Article
VL - 104
SP - 1373
EP - 1378
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 8
ER -