Abstract
Background: Despite evident success of malaria control in many sites in the Arabian Peninsula, malaria remains endemic in a few spots, in Yemen and south-west of Saudi Arabia. In addition to local transmission, imported malaria sustains an extra source of parasites that can challenge the strengths of local control strategies. This study examined the genetic diversity of Plasmodium falciparum in Yemen and mutations of drug resistant genes, to elucidate parasite structure and distribution of drug resistance genotypes in the region. Methods. Five polymorphic loci (MSP-2, Pfg377 and three microsatellites on chromosome 8) not involved in anti-malarial drug resistance, and four drug resistant genes (pfcrt, pfmdr1, dhfr and dhps) were genotyped in 108 P. falciparum isolates collected in three sites in Yemen: Dhamar, Hodeidah and Taiz. Results: High diversity was seen in non-drug genes, pfg377 (He = 0.66), msp-2 (He = 0.80) and three microsatellites on chr 8, 7.7 kb (He = 0.88), 4.3 kb (He = 0.77) and 0.8 kb (He = 0.71). There was a high level of mixed-genotype infections (57%), with an average 1.8 genotypes per patient. No linkage disequilibrium was seen between drug resistant genes and the non-drug markers (p <0.05). Genetic differentiation between populations was low (most pair-wise F ssub ST esub values
| Original language | English |
|---|---|
| Article number | 244 |
| Journal | Malaria Journal |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- Arabian Peninsula
- Drug resistance
- Genetic diversity
- Malaria
- Plasmodium falciparum
- Yemen
ASJC Scopus subject areas
- Infectious Diseases
- Parasitology
- Medicine(all)
Cite this
Genetic diversity of Plasmodium falciparum and distribution of drug resistance haplotypes in Yemen. / Al-Hamidhi, Salama; Mahdy, Mohammed Ak; Al-Hashami, Zainab; Al-Farsi, Hissa; Al-Mekhlafi, Abdulsalam M.; Idris, Mohamed A.; Beja-Pereira, Albano; Babiker, Hamza A.
In: Malaria Journal, Vol. 12, No. 1, 244, 2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genetic diversity of Plasmodium falciparum and distribution of drug resistance haplotypes in Yemen
AU - Al-Hamidhi, Salama
AU - Mahdy, Mohammed Ak
AU - Al-Hashami, Zainab
AU - Al-Farsi, Hissa
AU - Al-Mekhlafi, Abdulsalam M.
AU - Idris, Mohamed A.
AU - Beja-Pereira, Albano
AU - Babiker, Hamza A.
PY - 2013
Y1 - 2013
N2 - Background: Despite evident success of malaria control in many sites in the Arabian Peninsula, malaria remains endemic in a few spots, in Yemen and south-west of Saudi Arabia. In addition to local transmission, imported malaria sustains an extra source of parasites that can challenge the strengths of local control strategies. This study examined the genetic diversity of Plasmodium falciparum in Yemen and mutations of drug resistant genes, to elucidate parasite structure and distribution of drug resistance genotypes in the region. Methods. Five polymorphic loci (MSP-2, Pfg377 and three microsatellites on chromosome 8) not involved in anti-malarial drug resistance, and four drug resistant genes (pfcrt, pfmdr1, dhfr and dhps) were genotyped in 108 P. falciparum isolates collected in three sites in Yemen: Dhamar, Hodeidah and Taiz. Results: High diversity was seen in non-drug genes, pfg377 (He = 0.66), msp-2 (He = 0.80) and three microsatellites on chr 8, 7.7 kb (He = 0.88), 4.3 kb (He = 0.77) and 0.8 kb (He = 0.71). There was a high level of mixed-genotype infections (57%), with an average 1.8 genotypes per patient. No linkage disequilibrium was seen between drug resistant genes and the non-drug markers (p <0.05). Genetic differentiation between populations was low (most pair-wise F ssub ST esub values
AB - Background: Despite evident success of malaria control in many sites in the Arabian Peninsula, malaria remains endemic in a few spots, in Yemen and south-west of Saudi Arabia. In addition to local transmission, imported malaria sustains an extra source of parasites that can challenge the strengths of local control strategies. This study examined the genetic diversity of Plasmodium falciparum in Yemen and mutations of drug resistant genes, to elucidate parasite structure and distribution of drug resistance genotypes in the region. Methods. Five polymorphic loci (MSP-2, Pfg377 and three microsatellites on chromosome 8) not involved in anti-malarial drug resistance, and four drug resistant genes (pfcrt, pfmdr1, dhfr and dhps) were genotyped in 108 P. falciparum isolates collected in three sites in Yemen: Dhamar, Hodeidah and Taiz. Results: High diversity was seen in non-drug genes, pfg377 (He = 0.66), msp-2 (He = 0.80) and three microsatellites on chr 8, 7.7 kb (He = 0.88), 4.3 kb (He = 0.77) and 0.8 kb (He = 0.71). There was a high level of mixed-genotype infections (57%), with an average 1.8 genotypes per patient. No linkage disequilibrium was seen between drug resistant genes and the non-drug markers (p <0.05). Genetic differentiation between populations was low (most pair-wise F ssub ST esub values
KW - Arabian Peninsula
KW - Drug resistance
KW - Genetic diversity
KW - Malaria
KW - Plasmodium falciparum
KW - Yemen
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U2 - 10.1186/1475-2875-12-244
DO - 10.1186/1475-2875-12-244
M3 - Article
VL - 12
JO - Malaria Journal
JF - Malaria Journal
SN - 1475-2875
IS - 1
M1 - 244
ER -