TY - JOUR
T1 - Genetic diversity and prevalence of CCR2-CCR5 gene polymorphisms in the Omani population
AU - Al-Mahruqi, Samira H.
AU - Zadjali, Fahad
AU - Beja-Pereira, Albano
AU - Koh, Crystal Y.
AU - Balkhair, Abdullah
AU - Al-Jabri, Ali A.
PY - 2014
Y1 - 2014
N2 - Polymorphisms in the regulatory region of the CCR5 gene affect protein expression and modulate the progress of HIV-1 disease. Because of this prominent role, variations in this gene have been under differential pressure and their frequencies vary among human populations. The CCR2V64I mutation is tightly linked to certain polymorphisms in the CCR5 gene. The current Omani population is genetically diverse, a reflection of their history as traders who ruled extensive regions around the Indian Ocean. In this study, we examined the CCR2-CCR5 haplotypes in Omanis and compared the patterns of genetic diversity with those of other populations. Blood samples were collected from 115 Omani adults and genomic DNA was screened to identify the polymorphic sites in the CCR5 gene and the CCR2V64I mutation. Four minor alleles were common: CCR5-2554T and CCR5-2086G showed frequencies of 49% and 46%, respectively, whereas CCR5-2459A and CCR5-2135C both had a frequency of 36%. These alleles showed moderate levels of heterozygosity, indicating that they were under balancing selection. However, the well-known allele CCR5 32 was relatively rare. Eleven haplotypes were identified, four of which were common: HHC (46%), HHE (20%), HHA (14%) and HHF*2 (12%).
AB - Polymorphisms in the regulatory region of the CCR5 gene affect protein expression and modulate the progress of HIV-1 disease. Because of this prominent role, variations in this gene have been under differential pressure and their frequencies vary among human populations. The CCR2V64I mutation is tightly linked to certain polymorphisms in the CCR5 gene. The current Omani population is genetically diverse, a reflection of their history as traders who ruled extensive regions around the Indian Ocean. In this study, we examined the CCR2-CCR5 haplotypes in Omanis and compared the patterns of genetic diversity with those of other populations. Blood samples were collected from 115 Omani adults and genomic DNA was screened to identify the polymorphic sites in the CCR5 gene and the CCR2V64I mutation. Four minor alleles were common: CCR5-2554T and CCR5-2086G showed frequencies of 49% and 46%, respectively, whereas CCR5-2459A and CCR5-2135C both had a frequency of 36%. These alleles showed moderate levels of heterozygosity, indicating that they were under balancing selection. However, the well-known allele CCR5 32 was relatively rare. Eleven haplotypes were identified, four of which were common: HHC (46%), HHE (20%), HHA (14%) and HHF*2 (12%).
KW - CCR2
KW - CCR5
KW - Divergence
KW - HIV-1
KW - Haplotype
UR - http://www.scopus.com/inward/record.url?scp=84897134577&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897134577&partnerID=8YFLogxK
U2 - 10.1590/S1415-47572014000100004
DO - 10.1590/S1415-47572014000100004
M3 - Article
AN - SCOPUS:84897134577
SN - 1415-4757
VL - 37
SP - 7
EP - 14
JO - Genetics and Molecular Biology
JF - Genetics and Molecular Biology
IS - 1
ER -