TY - JOUR
T1 - Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes
AU - Tapaszti, Zsuzsanna
AU - Forgách, Petra
AU - Kovágó, Csaba
AU - Topolska, Grazyna
AU - Nowotny, Norbert
AU - Rusvai, Miklós
AU - Bakonyi, Tamás
N1 - Funding Information:
This study was supported by the Hungarian National Grants OTKA F043155, D048647, and M027651; the Hungarian-Slovenian Intergovernmental S&T Cooperation Grant OMFB-00482/07, and the Hungarian-Austrian Intergovernmental S&T Cooperation Grant OMFB-00738/07. T. Bakonyi is grantee of the “Bolyai János” Postdoctoral Fellowship Grant of the Hungarian Academy of Sciences.
PY - 2009/11/18
Y1 - 2009/11/18
N2 - Phylogenetic analysis of 22 Black queen cell virus (BQCV) genotypes collected from honeybee colonies in Poland, Austria and Hungary was performed on a partial helicase enzyme coding region (ORF1) and on a partial structural polypeptide coding region (ORF2). While the phylogeny based on the ORF2 region showed - with the exception of one strain from Poland - clustering of the genotypes corresponding to their geographic origin, the ORF1-based tree exhibited a completely different distribution of the Polish strains: three of them clustered within a branch clearly separated from all other central European BQCVs, while four other Polish strains remained well within the central European BQCV genotypes. In order to investigate this discrepancy in more detail, the nearly complete genome sequences of the three differing Polish strains were determined, together with one Hungarian sample. The sequences were aligned to each other and to the reference strain from South-Africa. Comparison of the different genome regions revealed that the 5′-UTR and the intergenic regions of the BQCV genome are highly conserved with longer homologous sections. ORF1 (non-structural protein coding region) was found more variable compared to ORF2 (structural protein coding region). The 5′-proximal third of ORF1 was particularly variable and contained several deletions/insertions. The sudden changes in the similarity levels of BQCV strains in different genomic regions are indicative of preceding recombination events.
AB - Phylogenetic analysis of 22 Black queen cell virus (BQCV) genotypes collected from honeybee colonies in Poland, Austria and Hungary was performed on a partial helicase enzyme coding region (ORF1) and on a partial structural polypeptide coding region (ORF2). While the phylogeny based on the ORF2 region showed - with the exception of one strain from Poland - clustering of the genotypes corresponding to their geographic origin, the ORF1-based tree exhibited a completely different distribution of the Polish strains: three of them clustered within a branch clearly separated from all other central European BQCVs, while four other Polish strains remained well within the central European BQCV genotypes. In order to investigate this discrepancy in more detail, the nearly complete genome sequences of the three differing Polish strains were determined, together with one Hungarian sample. The sequences were aligned to each other and to the reference strain from South-Africa. Comparison of the different genome regions revealed that the 5′-UTR and the intergenic regions of the BQCV genome are highly conserved with longer homologous sections. ORF1 (non-structural protein coding region) was found more variable compared to ORF2 (structural protein coding region). The 5′-proximal third of ORF1 was particularly variable and contained several deletions/insertions. The sudden changes in the similarity levels of BQCV strains in different genomic regions are indicative of preceding recombination events.
KW - BQCV
KW - Black queen cell virus
KW - Honeybee
KW - Phylogenetic analysis
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=70349895327&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349895327&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2009.06.002
DO - 10.1016/j.vetmic.2009.06.002
M3 - Article
C2 - 19570624
AN - SCOPUS:70349895327
SN - 0378-1135
VL - 139
SP - 227
EP - 234
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -