Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes

Zsuzsanna Tapaszti, Petra Forgách, Csaba Kovágó, Grazyna Topolska, Norbert Nowotny, Miklós Rusvai, Tamás Bakonyi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Phylogenetic analysis of 22 Black queen cell virus (BQCV) genotypes collected from honeybee colonies in Poland, Austria and Hungary was performed on a partial helicase enzyme coding region (ORF1) and on a partial structural polypeptide coding region (ORF2). While the phylogeny based on the ORF2 region showed - with the exception of one strain from Poland - clustering of the genotypes corresponding to their geographic origin, the ORF1-based tree exhibited a completely different distribution of the Polish strains: three of them clustered within a branch clearly separated from all other central European BQCVs, while four other Polish strains remained well within the central European BQCV genotypes. In order to investigate this discrepancy in more detail, the nearly complete genome sequences of the three differing Polish strains were determined, together with one Hungarian sample. The sequences were aligned to each other and to the reference strain from South-Africa. Comparison of the different genome regions revealed that the 5′-UTR and the intergenic regions of the BQCV genome are highly conserved with longer homologous sections. ORF1 (non-structural protein coding region) was found more variable compared to ORF2 (structural protein coding region). The 5′-proximal third of ORF1 was particularly variable and contained several deletions/insertions. The sudden changes in the similarity levels of BQCV strains in different genomic regions are indicative of preceding recombination events.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalVeterinary Microbiology
Volume139
Issue number3-4
DOIs
Publication statusPublished - Nov 18 2009

Fingerprint

Black queen cell virus
genetic techniques and protocols
Genotype
Viruses
genotype
phylogeny
Genome
Poland
Open Reading Frames
Austria-Hungary
Intergenic DNA
genome
open reading frames
5' Untranslated Regions
Phylogeny
South Africa
Genetic Recombination
Cluster Analysis
honey bee colonies
structural proteins

Keywords

  • Black queen cell virus
  • BQCV
  • Honeybee
  • Phylogenetic analysis
  • RT-PCR

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Tapaszti, Z., Forgách, P., Kovágó, C., Topolska, G., Nowotny, N., Rusvai, M., & Bakonyi, T. (2009). Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes. Veterinary Microbiology, 139(3-4), 227-234. https://doi.org/10.1016/j.vetmic.2009.06.002

Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes. / Tapaszti, Zsuzsanna; Forgách, Petra; Kovágó, Csaba; Topolska, Grazyna; Nowotny, Norbert; Rusvai, Miklós; Bakonyi, Tamás.

In: Veterinary Microbiology, Vol. 139, No. 3-4, 18.11.2009, p. 227-234.

Research output: Contribution to journalArticle

Tapaszti, Z, Forgách, P, Kovágó, C, Topolska, G, Nowotny, N, Rusvai, M & Bakonyi, T 2009, 'Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes', Veterinary Microbiology, vol. 139, no. 3-4, pp. 227-234. https://doi.org/10.1016/j.vetmic.2009.06.002
Tapaszti, Zsuzsanna ; Forgách, Petra ; Kovágó, Csaba ; Topolska, Grazyna ; Nowotny, Norbert ; Rusvai, Miklós ; Bakonyi, Tamás. / Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes. In: Veterinary Microbiology. 2009 ; Vol. 139, No. 3-4. pp. 227-234.
@article{c84de40611f745dea07dc6d4bd0ab493,
title = "Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes",
abstract = "Phylogenetic analysis of 22 Black queen cell virus (BQCV) genotypes collected from honeybee colonies in Poland, Austria and Hungary was performed on a partial helicase enzyme coding region (ORF1) and on a partial structural polypeptide coding region (ORF2). While the phylogeny based on the ORF2 region showed - with the exception of one strain from Poland - clustering of the genotypes corresponding to their geographic origin, the ORF1-based tree exhibited a completely different distribution of the Polish strains: three of them clustered within a branch clearly separated from all other central European BQCVs, while four other Polish strains remained well within the central European BQCV genotypes. In order to investigate this discrepancy in more detail, the nearly complete genome sequences of the three differing Polish strains were determined, together with one Hungarian sample. The sequences were aligned to each other and to the reference strain from South-Africa. Comparison of the different genome regions revealed that the 5′-UTR and the intergenic regions of the BQCV genome are highly conserved with longer homologous sections. ORF1 (non-structural protein coding region) was found more variable compared to ORF2 (structural protein coding region). The 5′-proximal third of ORF1 was particularly variable and contained several deletions/insertions. The sudden changes in the similarity levels of BQCV strains in different genomic regions are indicative of preceding recombination events.",
keywords = "Black queen cell virus, BQCV, Honeybee, Phylogenetic analysis, RT-PCR",
author = "Zsuzsanna Tapaszti and Petra Forg{\'a}ch and Csaba Kov{\'a}g{\'o} and Grazyna Topolska and Norbert Nowotny and Mikl{\'o}s Rusvai and Tam{\'a}s Bakonyi",
year = "2009",
month = "11",
day = "18",
doi = "10.1016/j.vetmic.2009.06.002",
language = "English",
volume = "139",
pages = "227--234",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "3-4",

}

TY - JOUR

T1 - Genetic analysis and phylogenetic comparison of Black queen cell virus genotypes

AU - Tapaszti, Zsuzsanna

AU - Forgách, Petra

AU - Kovágó, Csaba

AU - Topolska, Grazyna

AU - Nowotny, Norbert

AU - Rusvai, Miklós

AU - Bakonyi, Tamás

PY - 2009/11/18

Y1 - 2009/11/18

N2 - Phylogenetic analysis of 22 Black queen cell virus (BQCV) genotypes collected from honeybee colonies in Poland, Austria and Hungary was performed on a partial helicase enzyme coding region (ORF1) and on a partial structural polypeptide coding region (ORF2). While the phylogeny based on the ORF2 region showed - with the exception of one strain from Poland - clustering of the genotypes corresponding to their geographic origin, the ORF1-based tree exhibited a completely different distribution of the Polish strains: three of them clustered within a branch clearly separated from all other central European BQCVs, while four other Polish strains remained well within the central European BQCV genotypes. In order to investigate this discrepancy in more detail, the nearly complete genome sequences of the three differing Polish strains were determined, together with one Hungarian sample. The sequences were aligned to each other and to the reference strain from South-Africa. Comparison of the different genome regions revealed that the 5′-UTR and the intergenic regions of the BQCV genome are highly conserved with longer homologous sections. ORF1 (non-structural protein coding region) was found more variable compared to ORF2 (structural protein coding region). The 5′-proximal third of ORF1 was particularly variable and contained several deletions/insertions. The sudden changes in the similarity levels of BQCV strains in different genomic regions are indicative of preceding recombination events.

AB - Phylogenetic analysis of 22 Black queen cell virus (BQCV) genotypes collected from honeybee colonies in Poland, Austria and Hungary was performed on a partial helicase enzyme coding region (ORF1) and on a partial structural polypeptide coding region (ORF2). While the phylogeny based on the ORF2 region showed - with the exception of one strain from Poland - clustering of the genotypes corresponding to their geographic origin, the ORF1-based tree exhibited a completely different distribution of the Polish strains: three of them clustered within a branch clearly separated from all other central European BQCVs, while four other Polish strains remained well within the central European BQCV genotypes. In order to investigate this discrepancy in more detail, the nearly complete genome sequences of the three differing Polish strains were determined, together with one Hungarian sample. The sequences were aligned to each other and to the reference strain from South-Africa. Comparison of the different genome regions revealed that the 5′-UTR and the intergenic regions of the BQCV genome are highly conserved with longer homologous sections. ORF1 (non-structural protein coding region) was found more variable compared to ORF2 (structural protein coding region). The 5′-proximal third of ORF1 was particularly variable and contained several deletions/insertions. The sudden changes in the similarity levels of BQCV strains in different genomic regions are indicative of preceding recombination events.

KW - Black queen cell virus

KW - BQCV

KW - Honeybee

KW - Phylogenetic analysis

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=70349895327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349895327&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2009.06.002

DO - 10.1016/j.vetmic.2009.06.002

M3 - Article

C2 - 19570624

AN - SCOPUS:70349895327

VL - 139

SP - 227

EP - 234

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -